AZ-23 (Synonyms: AZD1332)

Name: AZ-23
Brand: MedChemExpress
SKU: HY-15590
Rating: 5.0 (3 reviews)

製品番号: HY-15590 純度: 98.92%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

AZ-23 is an ATP-competitive and orally bioavailable Trk kinase A/B/C inhibitor with IC₅₀s of 2 nM (TrkA), 8 nM (TrkB), 24 nM (FGFR1), 52 nM (Flt3), 55 nM (Ret), 84 nM (MuSk), 99 nM (Lck), respectively.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

AZ-23 構造式

CAS 番号 : 915720-21-7

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 176	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 176	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$160	在庫あり	Estimated Time of Arrival: December 31
10 mg	$260	在庫あり	Estimated Time of Arrival: December 31
25 mg	$560	在庫あり	Estimated Time of Arrival: December 31
50 mg	$960	在庫あり	Estimated Time of Arrival: December 31
100 mg	$1650	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

顧客検証

Trk Receptor アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

TrkA TrkB TrkC Trk

生物活性
プロトコル
純度とドキュメンテーション
参考文献
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製品説明

IC₅₀ & Target^[1]

TrkA

2 nM (IC₅₀)

TrkB

8 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
BaF3	IC₅₀	0.02 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA F589L mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA F589L mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.02 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA F589L mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA F589L mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.02 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA F589L mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA F589L mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.059 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA G595R mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA G595R mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.059 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA G595R mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA G595R mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.059 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA G595R mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA G595R mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.13 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA G667C mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA G667C mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.13 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA G667C mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA G667C mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
BaF3	IC₅₀	0.13 3 Compound: AZ-23	Antiproliferative activity against mouse BaF3 cells harboring TRKA G667C mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring TRKA G667C mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
KM12	IC₅₀	0.021 3 Compound: AZ-23	Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
MCF-10A	IC₅₀	1.51 1 Compound: 1a, AZ-23	Cytotoxicity against human MCF10A cells overexpressing constitutively active TRKA Cytotoxicity against human MCF10A cells overexpressing constitutively active TRKA	[PMID: 24900538]
RD	IC₅₀	5.3 3 Compound: 8, AZ-23, NCGC00250381	Antiproliferative activity against human RD cells after 48 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human RD cells after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 23787099]
Sf9	IC₅₀	2.81 1 Compound: T1; AZ-23	Inhibition of TRKA E735A mutant (unknown origin) expressed in baculovirus infected sf9 cells assessed as kinase domain dimerization by HTRF assay Inhibition of TRKA E735A mutant (unknown origin) expressed in baculovirus infected sf9 cells assessed as kinase domain dimerization by HTRF assay	[PMID: 31699609]
KM12	IC₅₀	0.021 3 Compound: AZ-23	Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
Sf9	IC₅₀	2.81 1 Compound: T1; AZ-23	Inhibition of TRKA E735A mutant (unknown origin) expressed in baculovirus infected sf9 cells assessed as kinase domain dimerization by HTRF assay Inhibition of TRKA E735A mutant (unknown origin) expressed in baculovirus infected sf9 cells assessed as kinase domain dimerization by HTRF assay	[PMID: 31699609]
KM12	IC₅₀	0.021 3 Compound: AZ-23	Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK8 assay	[PMID: 35617855]
MCF-10A	IC₅₀	1.51 1 Compound: 1a, AZ-23	Cytotoxicity against human MCF10A cells overexpressing constitutively active TRKA Cytotoxicity against human MCF10A cells overexpressing constitutively active TRKA	[PMID: 24900538]
SJRH30	IC₅₀	1.8 3 Compound: 8, AZ-23, NCGC00250381	Antiproliferative activity against human Rh30 cells after 48 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human Rh30 cells after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 23787099]
RD	IC₅₀	5.3 3 Compound: 8, AZ-23, NCGC00250381	Antiproliferative activity against human RD cells after 48 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human RD cells after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 23787099]
MCF-10A	IC₅₀	2430 1 Compound: 1a, AZ-23	Cytotoxicity against human MCF10A cells Cytotoxicity against human MCF10A cells	[PMID: 24900538]
SJRH30	IC₅₀	1.8 3 Compound: 8, AZ-23, NCGC00250381	Antiproliferative activity against human Rh30 cells after 48 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human Rh30 cells after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 23787099]
RD	IC₅₀	5.3 3 Compound: 8, AZ-23, NCGC00250381	Antiproliferative activity against human RD cells after 48 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human RD cells after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 23787099]
MCF-10A	IC₅₀	2430 1 Compound: 1a, AZ-23	Cytotoxicity against human MCF10A cells Cytotoxicity against human MCF10A cells	[PMID: 24900538]
SJRH30	IC₅₀	1.8 3 Compound: 8, AZ-23, NCGC00250381	Antiproliferative activity against human Rh30 cells after 48 hrs by CellTiter-Glo luminescent assay Antiproliferative activity against human Rh30 cells after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 23787099]
Sf9	IC₅₀	2.81 1 Compound: T1; AZ-23	Inhibition of TRKA E735A mutant (unknown origin) expressed in baculovirus infected sf9 cells assessed as kinase domain dimerization by HTRF assay Inhibition of TRKA E735A mutant (unknown origin) expressed in baculovirus infected sf9 cells assessed as kinase domain dimerization by HTRF assay	[PMID: 31699609]

体外実験

AZ-23 potently and selectivity inhibits Trk phosphorylation in cells. AZ-23 potently inhibits Trk-mediated survival (EC₅₀ of 2 nM). AZ-23 Inhibits Trk-Dependent Survival in MCF10ATrkA-Δ and TF-1 Cell Lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

AZ-23 shows in vivo TrkA kinase inhibition and efficacy in mice following oral administration in a TrkA-driven allograft model and significant tumor growth inhibition in a Trk-expressing xenograft model of neuroblastoma^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

391.83

分子式

C₁₇H₁₉ClFN₇O

CAS 番号

915720-21-7

Appearance

Solid

Color

White to off-white

SMILES

CC(OC1=CC(NC2=NC(N[C@H](C3=NC=C(F)C=C3)C)=NC=C2Cl)=NN1)C

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 125 mg/mL (319.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5521 mL	12.7606 mL	25.5213 mL
5 mM	0.5104 mL	2.5521 mL	5.1043 mL
10 mM	0.2552 mL	1.2761 mL	2.5521 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 98.92%

Select Batch:

データシート (272 KB) SDS (393 KB)

COA (246 KB) HNMR (146 KB) LCMS (94 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Thress K, et al. Identification and preclinical characterization of AZ-23, a novel, selective, and orally bioavailable inhibitor of the Trk kinase pathway. Mol Cancer Ther. 2009 Jul; 8(7):1818-27. [Content Brief]

細胞実験 ^[1]	Exponentially growing TF-1 cells are treated with various concentrations of AZ-23 and then incubated for an additional 72 h at 37°C in either growth or basal medium plus 100 ng/mL NGF. Cell proliferation is measured using MTS solution^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[1]	Mice^[1] Tumor-bearing mice are given a single, oral dose of compound and individual mice are sacrificed at various time points postdose (2, 6, 16, or 24 hours). Tumors are excised and homogenized and the resulting tumor lysates are analyzed using an ELISA for pTrkA^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Thress K, et al. Identification and preclinical characterization of AZ-23, a novel, selective, and orally bioavailable inhibitor of the Trk kinase pathway. Mol Cancer Ther. 2009 Jul; 8(7):1818-27. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5521 mL	12.7606 mL	25.5213 mL	63.8032 mL
	5 mM	0.5104 mL	2.5521 mL	5.1043 mL	12.7606 mL
	10 mM	0.2552 mL	1.2761 mL	2.5521 mL	6.3803 mL
	15 mM	0.1701 mL	0.8507 mL	1.7014 mL	4.2535 mL
	20 mM	0.1276 mL	0.6380 mL	1.2761 mL	3.1902 mL
	25 mM	0.1021 mL	0.5104 mL	1.0209 mL	2.5521 mL
	30 mM	0.0851 mL	0.4254 mL	0.8507 mL	2.1268 mL
	40 mM	0.0638 mL	0.3190 mL	0.6380 mL	1.5951 mL
	50 mM	0.0510 mL	0.2552 mL	0.5104 mL	1.2761 mL
	60 mM	0.0425 mL	0.2127 mL	0.4254 mL	1.0634 mL
	80 mM	0.0319 mL	0.1595 mL	0.3190 mL	0.7975 mL
	100 mM	0.0255 mL	0.1276 mL	0.2552 mL	0.6380 mL

AZ-23 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AZ-23915720-21-7AZD1332AZ23AZ 23AZD 1332AZD-1332Trk ReceptorTropomyosin related kinase receptorInhibitorinhibitorinhibit

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AZ-23 (Synonyms: AZD1332)

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参考文献

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AZ-23 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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AZ-23 (Synonyms: AZD1332)

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AZ-23 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Trk Receptor アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

AZ-23 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AZ-23 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report