2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. DUB-IN-2

DUB-IN-2 is a potent deubiquitinase inhibitor with an IC50 of 0.28 μM for USP8.

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CAS 番号 : 924296-19-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 99 在庫あり
Solution
10 mM * 1 mL in DMSO USD 99 在庫あり
Solid
5 mg $90 在庫あり
10 mg $169 在庫あり
25 mg $390 在庫あり
50 mg $663 在庫あり
100 mg $1060 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 20 publication(s) in Google Scholar

Other Forms of DUB-IN-2:

DUB-IN-2 関連抗体

Top Publications Citing Use of Products

    DUB-IN-2 purchased from MedChemExpress. Usage Cited in: Endocr J. 2020 Feb 28;67(2):177-184.  [Abstract]

    Effects of DUBs-IN-2 on EGFR protein expression in AtT-20 corticotroph tumor cells.Time-dependent effects of DUBs-IN-2 on EGFR protein expression. AtT-20 cells are incubated for 2, 6, and 24 h with 10 μM DUBs-IN-2, and western blot analysis was performed to examine EGFR protein levels. Dose-dependent effects of DUBs-IN-2 on EGFR protein expression. AtT-20 cells are incubated for 24 h with 0.1-10 μM of DUBs-IN-2.

    Deubiquitinase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15 USP28 USP25

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DUB-IN-2 is a potent deubiquitinase inhibitor with an IC50 of 0.28 μM for USP8[1].

    IC50 & Target

    IC50: 0.28 μM (USP8)[1]
    体外実験

    DUBs-IN-2 (compound 22 e) is a potent USP8 inhibitor with an IC50 of 0.28 μM, and has no effect on USP7, with an IC50 of >100 μM. DUBs-IN-2 inhibits the viability of HCT116 colon cell line and PC-3 prostate cancer cell line with IC50 values of 0.5-1.5 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DUB-IN-2 関連抗体
    分子量

    275.26

    分子式

    C15H9N5O
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    N#CC1=C(C#N)N=C2C(/C(C3=C2C=CC=C3)=N/OCC)=N1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 16.67 mg/mL (60.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6329 mL 18.1646 mL 36.3293 mL
    5 mM 0.7266 mL 3.6329 mL 7.2659 mL
    10 mM 0.3633 mL 1.8165 mL 3.6329 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
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    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 1 mg/mL (3.63 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 1.5 mg/mL (5.45 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6329 mL 18.1646 mL 36.3293 mL 90.8232 mL
    5 mM 0.7266 mL 3.6329 mL 7.2659 mL 18.1646 mL
    10 mM 0.3633 mL 1.8165 mL 3.6329 mL 9.0823 mL
    15 mM 0.2422 mL 1.2110 mL 2.4220 mL 6.0549 mL
    20 mM 0.1816 mL 0.9082 mL 1.8165 mL 4.5412 mL
    25 mM 0.1453 mL 0.7266 mL 1.4532 mL 3.6329 mL
    30 mM 0.1211 mL 0.6055 mL 1.2110 mL 3.0274 mL
    40 mM 0.0908 mL 0.4541 mL 0.9082 mL 2.2706 mL
    50 mM 0.0727 mL 0.3633 mL 0.7266 mL 1.8165 mL
    60 mM 0.0605 mL 0.3027 mL 0.6055 mL 1.5137 mL
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    DUB-IN-2 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DUB-IN-2924296-19-5DeubiquitinaseDUBsInhibitorinhibitorinhibit

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    製品名:
    DUB-IN-2
    製品番号:
    HY-50737A
    数量:
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