2. Metabolic Enzyme/Protease
  3. NADPH Oxidase
  4. GLX351322

GLX351322 is an inhibitor of NADPH oxidase 4 (Nox4), and inhibits hydrogen peroxide production from NOX4-overexpressing cells with an IC50 of 5 μM.

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CAS No. : 835598-94-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
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5 mg En stock
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Based on 47 publication(s) in Google Scholar

Other Forms of GLX351322:

GLX351322 Related Antibodies

Top Publications Citing Use of Products

47 Publications Citing Use of MCE GLX351322

Bio/Physico-chemical Assay
IF
Flow Cytometry
Cell Proliferation/Viability Assay
Cell Imaging/Staining
WB

    GLX351322 purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2025 Apr 9;33(4):529-544.e9.  [Abstract]

    GLX351322 (10 μM; 6 h) impaired IFN-I-mediated control of P. berghei infection in primary mouse (B6) hepatocyte cultures.

    GLX351322 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e13299.  [Abstract]

    Representative immunofluorescence images of ROS in C166 cells subjected to 0.4 mM H2O2 following NOX4 enzyme inhibition by GLX351322 (20 µM; 24 h) .

    GLX351322 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e13299.  [Abstract]

    Flow cytometry quantification of ROS in C166 cells subjected to 0.4 mM H2O2 following NOX4 enzyme inhibition by GLX351322 (20 µM; 24 h).

    GLX351322 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e13299.  [Abstract]

    Cell viability of C166 cells subjected to 0.4 mM H2O2 with or without GLX351322 (20 µM; 24 h) pretreatment.

    GLX351322 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e13299.  [Abstract]

    GLX351322 (20 µM). Assessment of intracellular ROS levels in C166 cells by fluorescence microscopy (DCFH-DA staining) following H2O2 challenge.

    GLX351322 purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Feb;24(4):2552-2565.  [Abstract]

    GLX351322 on the level of phospho-p38 MAPK in VCR-treated cells (*P < .05, NAC/VCR-treated cells compared to VCR-treated cells; GLX351322/VCR-treated cells compared to VCR-treated cells).

    Voir tous les produits spécifiques à Isoform NADPH Oxidase:

    Voir toutes les isoformes
    NOX1 NOX2 NOX4 NOX5 DUOX1

    • Activité biologique

    • Pureté et documentation

    • Références

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    Description

    GLX351322 is an inhibitor of NADPH oxidase 4 (Nox4), and inhibits hydrogen peroxide production from NOX4-overexpressing cells with an IC50 of 5 μM.

    IC50 & Target

    NOX4

     

    In Vitro

    GLX351322 is an inhibitor of NADPH oxidase 4, and inhibits hydrogen peroxide production from NOX4-overexpressing cells with an IC50 of 5 μM. GLX351322 shows weak activity against NOX2 in hPBMC cells (IC50, 40 μM).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    GLX351322 Related Antibodies
    In Vivo

    GLX351322 (3.8 mg/kg/day, p.o.) ameliorates HFD-induced hyperglycemia in mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Masse moléculaire

    431.51

    Formule

    C21H25N3O5S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC)C1=C(SC2=C1CCC2)NC(CN3CCN(CC3)C(C4=CC=CO4)=O)=O
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage
    Powder -20°C 3 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvant et solubilité
    In Vitro: 

    DMSO : 20 mg/mL (46.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3174 mL 11.5872 mL 23.1744 mL
    5 mM 0.4635 mL 2.3174 mL 4.6349 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1 mg/mL (2.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1 mg/mL (2.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 25 mg/mL (57.94 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3174 mL 11.5872 mL 23.1744 mL 57.9361 mL
    5 mM 0.4635 mL 2.3174 mL 4.6349 mL 11.5872 mL
    10 mM 0.2317 mL 1.1587 mL 2.3174 mL 5.7936 mL
    15 mM 0.1545 mL 0.7725 mL 1.5450 mL 3.8624 mL
    20 mM 0.1159 mL 0.5794 mL 1.1587 mL 2.8968 mL
    25 mM 0.0927 mL 0.4635 mL 0.9270 mL 2.3174 mL
    30 mM 0.0772 mL 0.3862 mL 0.7725 mL 1.9312 mL
    40 mM 0.0579 mL 0.2897 mL 0.5794 mL 1.4484 mL
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    GLX351322 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    GLX351322835598-94-2GLX 351322GLX-351322NADPH OxidaseNOXInhibitorinhibitorinhibit

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