1. GPCR/G Protein
    Neuronal Signaling
    Cell Cycle/DNA Damage
    Protein Tyrosine Kinase/RTK
  2. 5-HT Receptor
    RAD51
    DYRK

Harmine (Synonyms: Telepathine)

Cat. No.: HY-N0737A Purity: 99.58%
Handling Instructions

Harmine is a natural dual-specificity tyrosine phosphorylation-regulated kinase ((DYRK)) inhibitor with anticancer and anti-inflammatory activities.

For research use only. We do not sell to patients.

Harmine Chemical Structure

Harmine Chemical Structure

CAS No. : 442-51-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
500 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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5 g   Get quote  

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Customer Review

    Harmine purchased from MCE. Usage Cited in: Prog Neuropsychopharmacol Biol Psychiatry. 2017 Jun 15;79(Pt B):258-267.

    Representative images showing the restoration effect of Harmine on CUS-induced decrease in hippocampal DCX protein expressions. The Fluoxetine administration (20 mg/kg) is used as a positive control, and all data are shown as mean±SEM.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Harmine is a natural dual-specificity tyrosine phosphorylation-regulated kinase ((DYRK)) inhibitor with anticancer and anti-inflammatory activities.

    IC50 & Target

    5-HT2A Receptor

    397 nM (Ki)

    DYRK1A

     

    RAD51

     

    In Vitro

    Harmine is an inhibitor of 5-HT2A, with an Ki of 397 nM[1]. Harmine inhibits tau phosphorylation by DYRK1A by selected DANDYs, with an IC50 of 190 nM[2].Harmine negatively regulates HR by interfering Rad51 recruitment, resulting in severe cytotoxicity in hepatoma cells. Furthermore, NHEJ inhibitor Nu7441 markedly sensitizes Hep3B cells to the anti-proliferative effects of Harmine[3].

    In Vivo

    It is shown that brain water content is significantly increased in the TBI group. Treatment with Harmine significantly reduces the tissue water content at 1, 3 and 5 days, compared with the TBI group. Harmine treatment significantly reduces the escape latency at 3 and 5 days, compared with the TBI group. Post-TBI administration of Harmine significantly improves the motor function recovery of the rats at 1, 3 and 5 days following TBI, compared with the TBI group without Harmine treatment. The neuronal survival rate in the Harmine-treated group is significantly increased, compared with the TBI group. Administration of Harmine results in marked elevation in the expression of GLT-1, compared with the TBI group. The administration of Harmine significantly reduces the expression of caspase 3, compared with the TBI group[4].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (141.34 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7114 mL 23.5571 mL 47.1143 mL
    5 mM 0.9423 mL 4.7114 mL 9.4229 mL
    10 mM 0.4711 mL 2.3557 mL 4.7114 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

    References
    Animal Administration
    [4]

    Rats[4]
    A total of 150 male Sprague-Dawley rats (age, 10-12 weeks; weighing, 280-320 g; are used in the present study. The rats are randomly divided into three groups: Sham-operated group (sham; n=15); the TBI group (TBI; n=35) and the TBI + Harmine-treated group (Harmine; n=35). Harmine is administered immediately following TBI (i.p, 30 mg/kg per day) for up to 5 days. The sham and TBI groups receive equal volumes of 0.9% saline solution (i.p.). The rats are grouped as follows for examination of behavioral recovery: Sham, n=3; TBI, n=7; and Harmine, n=7. Following TBI, the NSS is evaluated at 1, 3 and 5 days. Each rat is assessed by an observer who is blinded to the animal treatment[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    212.25

    Formula

    C₁₃H₁₂N₂O

    CAS No.

    442-51-3

    SMILES

    CC1=NC=CC2=C1NC3=C2C=CC(OC)=C3

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.58%

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    Product Name:
    Harmine
    Cat. No.:
    HY-N0737A
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    Harmine

    Cat. No.: HY-N0737A