1. GPCR/G Protein
    Neuronal Signaling
    Cell Cycle/DNA Damage
    Protein Tyrosine Kinase/RTK
  2. 5-HT Receptor
    RAD51
    DYRK

Harmine (Synonyms: Telepathine)

Cat. No.: HY-N0737A Purity: 99.58%
Handling Instructions

Harmine, an inhibitor of DYRK1A and 5-HT2A, with a Ki of 397 nM for 5-HT2A, could also negatively regulate HR by interfering Rad51 recruitment.

For research use only. We do not sell to patients.

Harmine Chemical Structure

Harmine Chemical Structure

CAS No. : 442-51-3

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500 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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Customer Review

    Harmine purchased from MCE. Usage Cited in: Sci Rep. 2015 Aug 3;5:12728.

    Ganetespib decreases the DYRK1A protein level. 293T cells are transiently transfected with an expression vector for 3xFLAG-DYRK1A. At 24 h after transfection, the cells are treated with Ganetespib (100 nM) and collected 0 and 8 h after treatment. Total cell lysates are subjected to SDS-PAGE followed by Western blot analysis using antibodies against FLAG and GAPDH. In the control group (DMSO), expression of 3xFLAG-DYRK1A increases at 8 h compared to 0 h, and Ganetespib suppresses this increase of

    Harmine purchased from MCE. Usage Cited in: Prog Neuropsychopharmacol Biol Psychiatry. 2017 Jun 15;79(Pt B):258-267.

    Representative images showing the restoration effect of Harmine on CUS-induced decrease in hippocampal DCX protein expressions. The Fluoxetine administration (20 mg/kg) is used as a positive control, and all data are shown as mean±SEM.
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    Description

    Harmine, an inhibitor of DYRK1A and 5-HT2A, with a Ki of 397 nM for 5-HT2A, could also negatively regulate HR by interfering Rad51 recruitment.

    IC50 & Target

    5-HT2A Receptor

    397 nM (Ki)

    DYRK1A

     

    RAD51

     

    In Vitro

    Harmine is an inhibitor of 5-HT2A, with an Ki of 397 nM[1]. Harmine inhibits tau phosphorylation by DYRK1A by selected DANDYs, with an IC50 of 190 nM[2].Harmine negatively regulates HR by interfering Rad51 recruitment, resulting in severe cytotoxicity in hepatoma cells. Furthermore, NHEJ inhibitor Nu7441 markedly sensitizes Hep3B cells to the anti-proliferative effects of Harmine[3].

    In Vivo

    It is shown that brain water content is significantly increased in the TBI group. Treatment with Harmine significantly reduces the tissue water content at 1, 3 and 5 days, compared with the TBI group. Harmine treatment significantly reduces the escape latency at 3 and 5 days, compared with the TBI group. Post-TBI administration of Harmine significantly improves the motor function recovery of the rats at 1, 3 and 5 days following TBI, compared with the TBI group without Harmine treatment. The neuronal survival rate in the Harmine-treated group is significantly increased, compared with the TBI group. Administration of Harmine results in marked elevation in the expression of GLT‑1, compared with the TBI group. The administration of Harmine significantly reduces the expression of caspase 3, compared with the TBI group[4].

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (47.11 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7114 mL 23.5571 mL 47.1143 mL
    5 mM 0.9423 mL 4.7114 mL 9.4229 mL
    10 mM 0.4711 mL 2.3557 mL 4.7114 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Animal Administration
    [4]

    Rats[4]
    A total of 150 male Sprague‑Dawley rats (age, 10-12 weeks; weighing, 280-320 g; are used in the present study. The rats are randomly divided into three groups: Sham‑operated group (sham; n=15); the TBI group (TBI; n=35) and the TBI + Harmine‑treated group (Harmine; n=35). Harmine is administered immediately following TBI (i.p, 30 mg/kg per day) for up to 5 days. The sham and TBI groups receive equal volumes of 0.9% saline solution (i.p.). The rats are grouped as follows for examination of behavioral recovery: Sham, n=3; TBI, n=7; and Harmine, n=7. Following TBI, the NSS is evaluated at 1, 3 and 5 days. Each rat is assessed by an observer who is blinded to the animal treatment[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    212.25

    Formula

    C₁₃H₁₂N₂O

    CAS No.

    442-51-3

    SMILES

    CC1=NC=CC2=C1NC3=C2C=CC(OC)=C3

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.58%

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    Product Name:
    Harmine
    Cat. No.:
    HY-N0737A
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    Harmine

    Cat. No.: HY-N0737A