1. Cell Cycle/DNA Damage
  2. Topoisomerase

Idarubicin hydrochloride (Synonyms: 4-Demethoxydaunorubicin hydrochloride)

Cat. No.: HY-17381 Purity: 99.82%
Handling Instructions

Idarubicin hydrochloride is an anthracycline antibiotic in the treatment of leukaemia and a DNA topoisomerase II inhibitor.

For research use only. We do not sell to patients.
Idarubicin hydrochloride Chemical Structure

Idarubicin hydrochloride Chemical Structure

CAS No. : 57852-57-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 93 In-stock
5 mg USD 79 In-stock
10 mg USD 138 In-stock
50 mg USD 436 In-stock
100 mg   Get quote  
200 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Idarubicin hydrochloride is an anthracycline antibiotic in the treatment of leukaemia and a DNA topoisomerase II inhibitor.

IC50 & Target

DNA topoisomerase II[1]

In Vitro

The IC50 of idarubicin is 3.3±0.4 ng/mL on MCF-7 monolayers and 7.9±1.1 ng/mL in multicellular spheroids[1]. Idarubicin has shown a greater cytotoxicity than daunorubicin or doxorubicin in various in vitro systems. This has been attributed to a better ability of idarubicin to induce the formation of topoisomerase II -mediated DNA breaks[2].Idarubicin is about 57.5-fold and 25-fold more active than doxorubicin and epirubicin, respectively[3]. Idarubicin produces a concentration-dependent reduction in cell growth, with an IC50 value of approximately 0.01 μM. Idarubicin produced a concentration-dependent inhibition of DNA synthesis[4].

Clinical Trial
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.8728 mL 9.3642 mL 18.7283 mL
5 mM 0.3746 mL 1.8728 mL 3.7457 mL
10 mM 0.1873 mL 0.9364 mL 1.8728 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Stock solutions of idarubicin hydrochloride is dissolved in distilled water (1 mg/mL). MCF-7 monolayer are exposed to idarubicin or its metabolite idarubicinol at 0.01, 0.1, 1, 10, 100, and 1000 ng/mL for 24 hours. Multicellular spheroids are exposed to the same range of idarubicin and idarubicinol concentration as monolayers (0.01-1000 ng/mL) for 24 h and, in separate experiments, at the drug concentration of 100 ng/mL for 6, 12, 24 and 48 h. The inhibition of cell proliferation is determined by counting the viable cells with an hemocytometer. Results are expressed as percentage of cell survival vs. control cultures[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







OC(C(C(C1=CC=CC=C21)=O)=C3C2=O)=C4[[email protected]](C[[email protected]@](C(C)=O)(O)CC4=C3O)O[[email protected]@](O[[email protected]@H](C)[[email protected]]5O)([H])C[[email protected]@H]5N.Cl


4°C, protect from light


Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Idarubicin hydrochloride
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