1. Cell Cycle/DNA Damage
    Autophagy
  2. Topoisomerase
    Autophagy
  3. Idarubicin hydrochloride

Idarubicin hydrochloride (Synonyms: 4-Demethoxydaunorubicin hydrochloride)

Cat. No.: HY-17381 Purity: 99.82%
Handling Instructions

Idarubicin hydrochloride is an anthracycline antileukemic drug. It inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription.

For research use only. We do not sell to patients.

Idarubicin hydrochloride Chemical Structure

Idarubicin hydrochloride Chemical Structure

CAS No. : 57852-57-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 78 In-stock
Estimated Time of Arrival: December 31
5 mg USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 115 In-stock
Estimated Time of Arrival: December 31
50 mg USD 364 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Based on 4 publication(s) in Google Scholar

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Description

Idarubicin hydrochloride is an anthracycline antileukemic drug. It inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription.

IC50 & Target

DNA topoisomerase II[1]

In Vitro

The IC50 of idarubicin is 3.3±0.4 ng/mL on MCF-7 monolayers and 7.9±1.1 ng/mL in multicellular spheroids[1]. Idarubicin has shown a greater cytotoxicity than daunorubicin or doxorubicin in various in vitro systems. This has been attributed to a better ability of idarubicin to induce the formation of topoisomerase II -mediated DNA breaks[2].Idarubicin is about 57.5-fold and 25-fold more active than doxorubicin and epirubicin, respectively[3]. Idarubicin produces a concentration-dependent reduction in cell growth, with an IC50 value of approximately 0.01 μM. Idarubicin produced a concentration-dependent inhibition of DNA synthesis[4].

Clinical Trial
Molecular Weight

533.95

Formula

C₂₆H₂₈ClNO₉

CAS No.

57852-57-0

SMILES

OC(C(C(C1=CC=CC=C21)=O)=C3C2=O)=C4[[email protected]](C[[email protected]@](C(C)=O)(O)CC4=C3O)O[[email protected]@](O[[email protected]@H](C)[[email protected]]5O)([H])C[[email protected]@H]5N.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (93.64 mM)

H2O : 6.67 mg/mL (12.49 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8728 mL 9.3642 mL 18.7283 mL
5 mM 0.3746 mL 1.8728 mL 3.7457 mL
10 mM 0.1873 mL 0.9364 mL 1.8728 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.68 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.68 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Stock solutions of idarubicin hydrochloride is dissolved in distilled water (1 mg/mL). MCF-7 monolayer are exposed to idarubicin or its metabolite idarubicinol at 0.01, 0.1, 1, 10, 100, and 1000 ng/mL for 24 hours. Multicellular spheroids are exposed to the same range of idarubicin and idarubicinol concentration as monolayers (0.01-1000 ng/mL) for 24 h and, in separate experiments, at the drug concentration of 100 ng/mL for 6, 12, 24 and 48 h. The inhibition of cell proliferation is determined by counting the viable cells with an hemocytometer. Results are expressed as percentage of cell survival vs. control cultures[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.82%

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Keywords:

Idarubicin4-DemethoxydaunorubicinTopoisomeraseAutophagyInhibitorinhibitorinhibit

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Product name:
Idarubicin hydrochloride
Cat. No.:
HY-17381
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