2. Metabolic Enzyme/Protease
  3. Indoleamine 2,3-Dioxygenase (IDO)
  4. Indoximod

Indoximod  (Synonyms: 1-Methyl-D-tryptophan; NLG-8189; 1-メチル-D-トリプトファン)

製品番号: HY-16724 純度: 99.54%
COA 取扱説明書 Technical Support

Indoximod (1-Methyl-D-tryptophan) is an orally active indoleamine 2,3-dioxygenase (IDO) pathway inhibitor. Indoximod acts as a Trp mimetic in regulating mTOR. Indoximod is an immunometabolic adjuvant used for the research of cancer.

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CAS 番号 : 110117-83-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
100 mg $30 在庫あり
250 mg $43 在庫あり
500 mg $80 在庫あり
1 g $110 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 11 publication(s) in Google Scholar

Other Forms of Indoximod:

Indoximod 関連抗体

Top Publications Citing Use of Products

    Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269.  [Abstract]

    Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) resulted in significant weight gain in the BLM-induced mouse model.

    Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269.  [Abstract]

    Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) markedly improved overall survival compared with the BLM-treated group.

    Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269.  [Abstract]

    Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) reversed visual pulmonary injury and collagen deposition, attenuated the increase in lung coefficient, and prevented the reduction in terminal body weight in the BLM-induced mouse model.

    Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269.  [Abstract]

    Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) reversed the BLM-induced upregulation of Pla2g2a, Ido1, Acta2, and Col1a1 gene expression.

    Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108.  [Abstract]

    Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) increased renal injury in IgAN mice.

    Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108.  [Abstract]

    Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) promoted mesangial proliferation and collagen fibrillogenesis.

    Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108.  [Abstract]

    Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) promoted glomerular IgA accumulation.

    Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108.  [Abstract]

    Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) increased the proportion of Th17 cells and decreased the proportion of Treg cells in the kidney tissue of IgAN mice.

    Indoleamine 2,3-Dioxygenase (IDO) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    IDO1 IDO2 IDO

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Indoximod (1-Methyl-D-tryptophan) is an orally active indoleamine 2,3-dioxygenase (IDO) pathway inhibitor. Indoximod acts as a Trp mimetic in regulating mTOR. Indoximod is an immunometabolic adjuvant used for the research of cancer[1][2].

    IC50 & Target[1]

    IDO

    19 μM (Ki)

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    >100 μM
    Compound: D-MT
    Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    		[PMID: 31954880]
    HeLa EC50
    110 μM
    Compound: L-1MT
    Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells using L-tryptophan as substrate after 24 hrs
    Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells using L-tryptophan as substrate after 24 hrs
    		[PMID: 28526475]
    HeLa IC50
    >100 μM
    Compound: D-MT
    Anticancer activity against human HeLa cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    Anticancer activity against human HeLa cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    		[PMID: 31954880]
    HUVEC IC50
    >100 μM
    Compound: D-MT
    Cytotoxicity against HUVEC cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    Cytotoxicity against HUVEC cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    		[PMID: 31954880]
    PC-3 IC50
    >100 μM
    Compound: D-MT
    Anticancer activity against human PC3 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    Anticancer activity against human PC3 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
    		[PMID: 31954880]
    U-87MG ATCC IC50
    148.9 μM
    Compound: 1-D-MT
    Inhibition of recombinant human IDO2 expressed in human U87MG cells assessed as reduction in kynurenine formation using L-tryptophan as substrate after 6 hrs by spectrophotometry
    Inhibition of recombinant human IDO2 expressed in human U87MG cells assessed as reduction in kynurenine formation using L-tryptophan as substrate after 6 hrs by spectrophotometry
    		[PMID: 27475108]
    体外実験

    The IDO inhibitor 1-methyl-tryptophan exists in two stereoisomers with potentially different biological properties. The L isomer is the more potent inhibitor of IDO activity using the purified enzyme and in HeLa cell–based assays. However, the D isomer is significantly more effective in reversing the suppression of T cells created by IDO-expressing dendritic cells. The L isomer of 1-methyl-tryptophan functioned as a competitive inhibitor (Ki=19 μM), whereas the d isomer is much less effective. The DL mixture is intermediate, with a Ki of 35 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Indoximod 関連抗体
    体内実験

    The D isomer is more efficacious as an anticancer agent in chemo-immunotherapy regimens using NSC-26271, NSC 125973, or LY 188011, when tested in mouse models of transplantable melanoma and transplantable and autochthonous breast cancer. The D isomer of 1-methyl-tryptophan specifically targets the IDO gene because the antitumor effect of d-1-methyl-tryptophan is completely lost in mice with a disruption of the IDO gene (IDO-knockout mice). Oral administration of dl-1-methyl-tryptophan in combination with NSC 125973 can elicit regression of autochthonous breast tumors[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    218.26

    分子式

    C12H14N2O2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@H](CC1=CN(C)C2=CC=CC=C12)C(O)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    0.5 M NaOH : 66.67 mg/mL (305.46 mM; Need ultrasonic)

    H2O : 5 mg/mL (22.91 mM; ultrasonic and adjust pH to 2 with HCl)

    DMSO : 0.55 mg/mL (2.52 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5817 mL 22.9085 mL 45.8169 mL
    5 mM 0.9163 mL 4.5817 mL 9.1634 mL
    10 mM 0.4582 mL 2.2908 mL 4.5817 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 1 mg/mL (4.58 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.85%

    COA (252 KB) HNMR (157 KB) RP-HPLC (258 KB) LCMS (91 KB)

    取扱説明書 (2659 KB)
    参考文献
    キナーゼ実験
    [1]

    1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 µM to wells containing the reaction mixture with tryptophan (0-200 µM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 µl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 µl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O / 0.5 M NaOH 1 mM 4.5817 mL 22.9085 mL 45.8169 mL 114.5423 mL
    H2O / 0.5 M NaOH 5 mM 0.9163 mL 4.5817 mL 9.1634 mL 22.9085 mL
    10 mM 0.4582 mL 2.2908 mL 4.5817 mL 11.4542 mL
    15 mM 0.3054 mL 1.5272 mL 3.0545 mL 7.6362 mL
    20 mM 0.2291 mL 1.1454 mL 2.2908 mL 5.7271 mL
    0.5 M NaOH 25 mM 0.1833 mL 0.9163 mL 1.8327 mL 4.5817 mL
    30 mM 0.1527 mL 0.7636 mL 1.5272 mL 3.8181 mL
    40 mM 0.1145 mL 0.5727 mL 1.1454 mL 2.8636 mL
    50 mM 0.0916 mL 0.4582 mL 0.9163 mL 2.2908 mL
    60 mM 0.0764 mL 0.3818 mL 0.7636 mL 1.9090 mL
    80 mM 0.0573 mL 0.2864 mL 0.5727 mL 1.4318 mL
    100 mM 0.0458 mL 0.2291 mL 0.4582 mL 1.1454 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Indoximod Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Indoximod110117-83-41-Methyl-D-tryptophan NLG-8189NLG8189NLG 8189NLG-8189Indoleamine 2,3-Dioxygenase (IDO)tryptophanTrp mimeticimmunotherapeutic vaccinesmTORC1cancerInhibitorinhibitorinhibit

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