2. Cell Cycle/DNA Damage Cytoskeleton Antibody-drug Conjugate/ADC Related
  3. Microtubule/Tubulin ADC Payload
  4. Mertansine

Mertansine  (Synonyms: DM1; Maytansinoid DM1)

製品番号: HY-19792 純度: 99.75%
COA 取扱説明書 Technical Support

Mertansine (DM1) is a microtubulin inhibitor and is an antibody-conjugatable maytansinoid that is developed to overcome systemic toxicity associated with maytansine and to enhance tumor-specific delivery. Mertansine can be attached to a monoclonal antibody with a linker to create an antibody-drug conjugate (ADC).

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CAS 番号 : 139504-50-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
2 mg $50 在庫あり
5 mg $80 在庫あり
10 mg $125 在庫あり
25 mg $190 在庫あり
50 mg $250 在庫あり
100 mg $400 在庫あり
250 mg $720 在庫あり
500 mg   お問い合わせ  
1 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 27 publication(s) in Google Scholar

Other Forms of Mertansine:

Mertansine 関連抗体

Top Publications Citing Use of Products

    Mertansine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 2;16(1):3167.  [Abstract]

    Various indicated cancer lines were seeded at low density and treated with titrated doses of DXd or DM1 (Mertansine, 0.1-10000 nM), with DMSO solvent used as control treatment group. Cells were cultured for 5 days or until control groups reached >90% confluency. Cell viability and drug induced toxicity were assessed by cellular ATP quantification using using CellTiter-Glo Luminescent Cell Viability Assay following manufacturer’s protocol. Nonlinear regression curve fit analysis were used to calculate IC50 values (n = 3 per group).

    Mertansine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 2;16(1):3167.  [Abstract]

    Flow cytometry assessment of surface CD47 expression on Au565 cells after 2 days of treatment with DXd (Deruxtecan, 10-250 nM) or DM1 (Mertansine, 10-250 nM).

    Mertansine purchased from MedChemExpress. Usage Cited in: J Control Release. 2024 Sep 19:375:438-453.  [Abstract]

    Cytotoxicity of DM1 (Mertansine, 50-1000 nM) against OVCAR5, OVCAR8, and ID8 Trp53−/− ovarian cancer cell lines was determined at 72 h (left). Standard-of-care chemotherapeutics Paclitaxel (center) and Doxorubicin (right) showed similar order-of-magnitude IC50, compared to DM1, at 72 h. (IC50DM1 ≈ 50 nM, IC50Paclitaxel ≈ 10 nM (on OVCAR5 & OVCAR8), IC50paclitaxel ≈ 500 nM (on ID8 Trp53−/−), and IC50Doxorubicin ≈ 50 nM)

    Mertansine purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2023 Aug 9;42(1):200.  [Abstract]

    HT29 and HCT116 cells were treated with the doses indicated of anti-CD98hc, anti-CD98hc-DM1 or Mertansine (DM1, 0.1-10 nM) for four days. The data are plotted as the percentage of MTT metabolization with respect to control.

    Mertansine purchased from MedChemExpress. Usage Cited in: Biomaterials. 2023 Jan:292:121913.

    Free Mertansine (DM1; 20 nM; 24 h) is able to induce apoptosis in NCI-H929 cells.

    ADC Payload アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Auristatin Calicheamicins Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Mertansine (DM1) is a microtubulin inhibitor and is an antibody-conjugatable maytansinoid that is developed to overcome systemic toxicity associated with maytansine and to enhance tumor-specific delivery. Mertansine can be attached to a monoclonal antibody with a linker to create an antibody-drug conjugate (ADC)[1][2].

    IC50 & Target

    Maytansinoids

     

    体外実験

    Mertansine is a strong antiproliferative chemotherapeutics toward over 60 types of cancer cell lines[3].
    Mertansine (0-1 μg/mL) shows antitumor activity in malignant B16F10 melanoma cells, and inhibits tumor cell growth by inhibiting mitosis when combined with DTX[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Mertansine 関連抗体

    Cell Proliferation Assay

    Cell Line: Malignant B16F10 melanoma cells[3]
    Concentration: 0, 0.01, 0.1, and 1 μg/mL
    Incubation Time: 4 h
    Result: Showed antitumor activity in malignant B16F10 melanoma cells, with an IC50 of 0.092 μg/mL. Co-delivery of DTX and DM1, both of which inhibit tumor cell growth by inhibiting mitosis, is an effective strategy to achieve a combinatorial anticancer effect.
    体内実験

    Mertansine (DM1) has a low maximum-tolerated dose (MTD) of 1 mg/kg[3]

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    738.29

    分子式

    C35H48ClN3O10S
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    C[C@]1([C@@](CC(N(C(C=C2C=C3OC)=C3Cl)C)=O)([H])OC([C@H](C)N(C)C(CCS)=O)=O)[C@H]([C@@H]([C@](OC4=O)([H])C[C@]([C@](/C=C/C=C(C)/C2)([H])OC)(N4)O)C)O1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years

    * The compound is unstable in solutions, freshly prepared is recommended.

    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (67.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3545 mL 6.7724 mL 13.5448 mL
    5 mM 0.2709 mL 1.3545 mL 2.7090 mL
    10 mM 0.1354 mL 0.6772 mL 1.3545 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 0.83 mg/mL (1.12 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 0.83 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 0.83 mg/mL (1.12 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *The compound is unstable in solutions, freshly prepared is recommended.

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.75%

    COA (259 KB) HNMR (304 KB) RP-HPLC (247 KB) LCMS (101 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.3545 mL 6.7724 mL 13.5448 mL 33.8620 mL
    5 mM 0.2709 mL 1.3545 mL 2.7090 mL 6.7724 mL
    10 mM 0.1354 mL 0.6772 mL 1.3545 mL 3.3862 mL
    15 mM 0.0903 mL 0.4515 mL 0.9030 mL 2.2575 mL
    20 mM 0.0677 mL 0.3386 mL 0.6772 mL 1.6931 mL
    25 mM 0.0542 mL 0.2709 mL 0.5418 mL 1.3545 mL
    30 mM 0.0451 mL 0.2257 mL 0.4515 mL 1.1287 mL
    40 mM 0.0339 mL 0.1693 mL 0.3386 mL 0.8466 mL
    50 mM 0.0271 mL 0.1354 mL 0.2709 mL 0.6772 mL
    60 mM 0.0226 mL 0.1129 mL 0.2257 mL 0.5644 mL
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    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Mertansine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mertansine139504-50-0DM1 Maytansinoid DM1DM 1DM-1Maytansinoid DM1Maytansinoid DM 1Maytansinoid DM-1Microtubule/TubulinADC PayloadADC CytotoxinB16F10MDA-MB-231melanomaantiproliferativeADCmitosisantitumortubulinInhibitorinhibitorinhibit

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    製品名:
    Mertansine
    製品番号:
    HY-19792
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