2. GPCR/G Protein
  3. Adenosine Receptor
  4. Norisoboldine

Norisoboldine  (Synonyms: (+)-Laurelliptine)

Cat. No.: HY-N0586 Purity: 99.44%
COA Handling Instructions

Norisoboldine is an orally active natural aryl hydrocarbon receptor (AhR) agonist. Norisoboldine, as a major isoquinoline alkaloid present in Radix Linderae, can be used for the research of Rheumatoid arthritis and Ulcerative colitis.

For research use only. We do not sell to patients.

Norisoboldine Chemical Structure

Norisoboldine Chemical Structure

CAS No. : 23599-69-1

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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Norisoboldine is an orally active natural aryl hydrocarbon receptor (AhR) agonist. Norisoboldine, as a major isoquinoline alkaloid present in Radix Linderae, can be used for the research of Rheumatoid arthritis and Ulcerative colitis[1][2].

IC50 & Target

AhR[2]
In Vitro

Norisoboldine (1~30 μM; 0~24 hours; CD4+T cells) activates AhR under hypoxic microenvironment and significantly downregulates mRNA expression of miR-31[2].
Norisoboldine (30 μM; 0~24 hours; CD4+T cells) inhibits glycolysis in hypoxia[2].
Norisoboldine (1~30 μM; 0~72 hours; Treg cells) promotes Treg differentiation in hypoxia[2].
Norisoboldine (10, 30 μM) facilitates the disassociation of HSP90/AhR complexes, the nuclear translocation of AhR, and the formation of AhR/ARNT complexes. Norisoboldine induces generation of Treg cells in hypoxia is independent of miR-31[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: CD4+T cells
Concentration: 1~30 μM
Incubation Time: 24 hours
Result: Activated AhR in cells under hypoxic microenvironment.

RT-PCR[2]

Cell Line: CD4+T cells
Concentration: 1~30 μM
Incubation Time: 24 hours
Result: Significantly downregulated mRNA expression of miR-31.

Immunofluorescence[2]

Cell Line: CD4+T cells
Concentration: 30 μM
Incubation Time: 24 hours
Result: Inhibited glycolysis in hypoxia.

Cell Differentiation Assay[2]

Cell Line: Treg cells
Concentration: 1~30 μM
Incubation Time: 72 hours
Result: Promoted Treg differentiation in hypoxia.
In Vivo

Norisoboldine (10~40 mg/kg; p.o.; 20 days) significantly reduces the severity of joint swelling and erythema during the course of the experiment[1].
Norisoboldine (40 mg/kg; i.g.; 10 days) induces enhancement of CYP1A1 expression and suppresses expressions of Glut1 and HK2 in colons[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice (18–22 g)
Dosage: 10~40 mg/kg
Administration: P.o.
Result: Significantly reduced the severity of joint swelling and erythema during the course of the experiment.
Animal Model: Female C57BL/6 mice (18–22 g)
Dosage: 40 mg/kg
Administration: I.g.
Result: Induced enhancement of CYP1A1 expression and suppressed expressions of Glut1 and HK2 in colons.
Molecular Weight

313.35

Appearance

Solid

Formula

C18H19NO4
CAS No.
SMILES

OC1=C(OC)C=C2CCN[[email protected]@]3([H])CC4=CC(O)=C(OC)C=C4C1=C23
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro: 

DMSO : ≥ 62.5 mg/mL (199.46 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1913 mL 15.9566 mL 31.9132 mL
5 mM 0.6383 mL 3.1913 mL 6.3826 mL
10 mM 0.3191 mL 1.5957 mL 3.1913 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.64 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.64 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Norisoboldine23599-69-1(+)-LaurelliptineAdenosine ReceptorP1 receptororallynaturalisoquinolinealkaloidradixlinderaearthritisUCDSSInhibitorinhibitorinhibit

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