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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1416
    HMBR
    		98.43%
    HMBR is an analogue with an additional methyl group on the aromatic ring and is non-fluorescent. HMBR conjugated with Y-FAST emits yellow fluorescence under blue light excitation (Ex= 419 nm; Em= 525–539 nm). HMBR is non-toxic to zebrafish embryos. HMBR has high cell permeability.
    HMBR
  • HY-137276
    5-Bromo-3-indolyl β-D-galactopyranoside
    		99.73%
    5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues.
    5-Bromo-3-indolyl β-D-galactopyranoside
  • HY-D1730A
    AF488 NHS ester TEA
    AF488 NHS ester TEA is an amine specific fluorescence probe (λem=525 nm=525 nm). AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 NHS ester TEA
  • HY-D0236
    Congo Red
    Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
    Congo Red
  • HY-111377
    Amine-PEG3-Biotin
    		99.79%
    Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group.
    Amine-PEG3-Biotin
  • HY-136865
    MeOSuc-AAPV-AMC
    		98.43%
    MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).
    MeOSuc-AAPV-AMC
  • HY-101891
    Di-8-ANEPPS
    		99.84%
    Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .
    Di-8-ANEPPS
  • HY-D1820
    Vari Fluor 594-Phalloidin
    Vari Fluor 594-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence at 585/609 nm when labeled.
    Vari Fluor 594-Phalloidin
  • HY-D0048
    5-TAMRA-SE
    5-TAMRA-SE is an amine-reactive fluorescent agent, and its conjugate produces bright, pH-insensitive orange-red fluorescence with good photostability (Ex/Em = 565/580 nm).
    5-TAMRA-SE
  • HY-D0988
    R-Phycoerythrin
    R-Phycoerythrin is found in Heterosiphonia japonica. R-Phycoerythrin is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex: 495 nm).
    R-Phycoerythrin
  • HY-136626
    Z-Ala-Ala-Asn-AMC
    		99.28%
    Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.
    Z-Ala-Ala-Asn-AMC
  • HY-134434
    Z-Arg-Arg-AMC hydrochloride
    		99.83%
    Z-Arg-Arg-AMC hydrochloride is a highly selective fluorescent Cathepsin B substrate. Z-Arg-Arg-AMC hydrochloride can be hydrolyzed by Cathepsin B to produce a fluorescent product for enzyme activity detection.
    Z-Arg-Arg-AMC hydrochloride
  • HY-D1449
    IR-1061
    IR-1061 is a near infrared fluorescent organic dye for in vivo bimodal imaging.
    IR-1061
  • HY-101895
    Fluorescein di(β-D-galactopyranoside)
    		99.81%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)
  • HY-150086
    Copper Fluor-4
    		98.44%
    Copper Fluor-4 (CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 has high copper selectivity with a Kd value of 2.9×10−13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).
    Copper Fluor-4
  • HY-P0019
    Z-Gly-Gly-Arg-AMC
    		99.98%
    Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
    Z-Gly-Gly-Arg-AMC
  • HY-D1269
    Rhodamine-N3 chloride
    		99.85%
    Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups. Rhodamine-N3 chloride is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 544/576 nm).
    Rhodamine-N3 chloride
  • HY-D1730
    AF488 NHS ester
    AF488 NHS ester is an amine specific fluorescence probe (Em=525 nm). AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization.
    AF488 NHS ester
  • HY-158082
    TRITC-dextran, MW 4000
    TRITC-dextran MW 4000 (Tetramethyl rhodamine isothiocyanate glucan, MW 4000) is a fluorescent dye, with the molecular weight of 4 kD. TRITC-dextran MW 4000 exhibits an excitation wavelength of 555 nm. TRITC-dextran MW 4000 is vessel penetrate, which could label blood plasma to visualize the vasculature. TRITC-dextran MW 4000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 4000
  • HY-D0069
    CPM
    		≥99.0%
    CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye (excitation/emission maxima of 387/463 nm).
    CPM
Cat. No. Product Name / Synonyms Application Reactivity