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  4. Rhodamine-N3 chloride

Rhodamine-N3 chloride 

Cat. No.: HY-D1269 Purity: 97.06%
COA Handling Instructions

Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups.

For research use only. We do not sell to patients.

Rhodamine-N3 chloride Chemical Structure

Rhodamine-N3 chloride Chemical Structure

CAS No. : 2363751-90-8

Size Price Stock Quantity
5 mg USD 350 In-stock
10 mg USD 580 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Rhodamine-N3 chloride

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups[1][2].

In Vitro

The cell lysates or cells are treated with probes (0.001-10 μM, 1 hr), and then reacted with an Rhodamine-N3 (N3-Rh) reporter tag under copper-catalyzed azide-alkyne cycloaddition (CuACC or click chemistry) conditions and probe-labeled proteins visualized by SDS-PAGE and in-gel fluorescence scanning[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

847.44

Appearance

Solid

Formula

C44H59ClN8O7
CAS No.
Emission (Em)

576
Excitation (Ex)

544
SMILES

[N-]=[N+]=NCCOCCOCCOCCNC(CCC(N1CCN(CC1)C(C2=C(C3=C4C=CC(N(CC)CC)=CC4=[O+]C5=C3C=CC(N(CC)CC)=C5)C=CC=C2)=O)=O)=O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

DMSO : 3.33 mg/mL (3.93 mM; ultrasonic and warming and heat to 60°C)

H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1800 mL 5.9001 mL 11.8002 mL
5 mM --- --- ---
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 97.06%

COA (243 KB) RP-HPLC (154 KB) MS (87 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Rhodamine-N32363751-90-8Fluorescent Dyeazide,rhodaminefluorescentdyealkyneInhibitorinhibitorinhibit

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