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  3. Fluorescent Dye
  4. Rhodamine-N3 chloride

Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups. Rhodamine-N3 (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

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Rhodamine-N3 chloride Chemical Structure

Rhodamine-N3 chloride Chemical Structure

CAS No. : 2363751-90-8

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Based on 1 publication(s) in Google Scholar

Rhodamine-N3 chloride Related Antibodies

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Description

Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups[1][2]. Rhodamine-N3 (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

In Vitro

The cell lysates or cells are treated with probes (0.001-10 μM, 1 hr), and then reacted with an Rhodamine-N3 (N3-Rh) reporter tag under copper-catalyzed azide-alkyne cycloaddition (CuACC or click chemistry) conditions and probe-labeled proteins visualized by SDS-PAGE and in-gel fluorescence scanning[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodamine-N3 chloride Related Antibodies
Molecular Weight

847.44

Formula

C44H59ClN8O7
CAS No.
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

576
Excitation (Ex)

544
SMILES

[N-]=[N+]=NCCOCCOCCOCCNC(CCC(N1CCN(CC1)C(C2=C(C3=C4C=CC(N(CC)CC)=CC4=[O+]C5=C3C=CC(N(CC)CC)=C5)C=CC=C2)=O)=O)=O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 3.33 mg/mL (3.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1800 mL 5.9001 mL 11.8002 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 97.06%

COA (244 KB) RP-HPLC (154 KB) MS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1800 mL 5.9001 mL 11.8002 mL 29.5006 mL
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Rhodamine-N3 chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine-N32363751-90-8Fluorescent Dyeazide,rhodaminefluorescentdyealkyneInhibitorinhibitorinhibit

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