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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W001952
    6-Bromo-2-naphthol
    		99.75%
    6-Bromo-2-naphthol is an RTP (real-time polymerase chain reaction) probe that forms a 1:1 or 2:1 complex with β-cyclodextrin (β-CD). 6-Bromo-2-naphthol is capable of real-time monitoring of PCR reactions and quantification of specific nucleic acid sequences. RTP probes are a class of small DNA or RNA sequences labeled with fluorescent dyes and quencher molecules, which can be widely used in gene expression analysis, SNP genotyping, and pathogen detection. 6-Bromo-2-naphthol embeds into the cyclodextrin cavity through hydrophobic interactions, inhibits the oxygen quenching effect, and emits a phosphorescent signal at room temperature. 6-Bromo-2-naphthol can also be used as an intermediate for the synthesis of antibacterial azo dyes, and its derivatives show antibacterial activity against Staphylococcus aureus, Escherichia coli and other bacteria.
    6-Bromo-2-naphthol
  • HY-W878943
    FAM azide, 5-isomer
    FAM azide, 5-isomer is a Click Chemistry labeling reagent.
    FAM azide, 5-isomer
  • HY-D1053
    CY7-N3
    CY7-N3 (Sulfo-Cyanine7-N3) is a water-soluble NIR dye azide for Click Chemistry. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    CY7-N3
  • HY-D0785
    NBD-F
    		98.87%
    NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent which is developed for amino acid analysis. NBD-F reacts with primary or secondary amines to produce a fluorescent product and used for analysis of amino acids and low molecular weight amines.
    NBD-F
  • HY-D2367
    Rhodamine B NHS ester
    		99.87%
    Rhodamine B NHS ester (N-Hydroxysuccinimidyl rhodamine B ester) is an amine-reactive fluorescent probe (λex/λem: 564/589 nm, respectively). Rhodamine B NHS ester could react with Glycine (HY-Y0966).
    Rhodamine B NHS ester
  • HY-D0054
    9-(2-Carboxy-2-cyanovinyl)julolidine
    		99.52%
    9-(2-Carboxy-2-cyanovinyl)julolidine is a fluorescent molecular rotor. 9-(2-Carboxy-2-cyanovinyl)julolidine can be used to bind to IgG and Fab, and preparing antibodies.
    9-(2-Carboxy-2-cyanovinyl)julolidine
  • HY-D1566
    Sulfo-Cy7.5 maleimide
    Sulfo-Cy7.5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Sulfo-Cy7.5 maleimide
  • HY-151644
    Fluorescein hydrazide
    		99.31%
    Fluorescein hydrazide is a sensitive fluorescent probe for Hg2+ and Co2+ that induces obvious color changes and fluorescence changes. Fluorescein hydrazide can be prepared by the reaction of fluorescein and hydrazine. Fluorescein hydrazide exhibits an excitation wavelength of 508 nM and an emission wavelength of 531 nM[2][3].
    Fluorescein hydrazide
  • HY-W017873
    9-Fluorenylmethyl carbazate
    		99.87%
    9-Fluorenylmethyl carbazate is used as a fluorophore reagent for a fluorimetric detection of glycans.
    9-Fluorenylmethyl carbazate
  • HY-126994
    6′-Amino-D-luciferin
    		98.45%
    6’-Amino-D-luciferin is a derivative of D-luciferin (HY-12591A) with an amino group replacing the 6-hydroxyl group. 6’-Amino-D-luciferin is a natural substrate of luciferase (Luc) and can be used for IVIS imaging in cell/animal experiments[1][2][3].
    6′-Amino-D-luciferin
  • HY-P4319
    Boc-Glu-Lys-Lys-AMC
    		99.95%
    Boc-Glu-Lys-Lys-AMC is a sensitive fluorogenic substrate for urokinase-activated plasmin.
    Boc-Glu-Lys-Lys-AMC
  • HY-118907
    Methyl Orange
    		99.85%
    Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.
    Methyl Orange
  • HY-139646
    KMG-104
    		99.12%
    KMG-104 is a cell-impermeant highly selective fluorescent Mg2+ probe. KMG-104 has been used widely and revealed Mg2+ mobilization in cytoplasm in various types of cells.
    KMG-104
  • HY-D0789
    ANTS
    		99.09%
    ANTS is a fluorescent dye (Excitation wavelength: 380 nm; Emission wavelength: 520 nm). ANTS and DPX are encapsulated in liposomes can be an effective approach for measuring membrane leakage.
    ANTS
  • HY-D1098
    SYBR Green II
    SYBR Green II is a fluorescent nucleic acid dye that mainly binds single-stranded nucleotides. SYBR Green II is sensitive to oligonucleotides or larger nucleic acid polymers in a variety of cells and gels. SYBR Green II can be used to study cell structure, membrane integrity or function, and cell cycle distribution. Wavelength 484/515 nm.
    SYBR Green II
  • HY-135414
    Cyanine5 NHS ester chloride
    		98.0%
    Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.
    Cyanine5 NHS ester chloride
  • HY-W040681
    7-Dimethylaminocoumarin-4-acetic acid
    		98.05%
    7-Dimethylaminocoumarin-4-acetic acid is a fluorescent probe.
    7-Dimethylaminocoumarin-4-acetic acid
  • HY-134435
    Z-Arg-AMC hydrochloride
    		99.2%
    Z-Arg-AMC hydrochloride is a fluorogenic substrate for trypsin and papain. Z-Arg-AMC hydrochloride can be used to research activity of trypsin (Ex/Em=355/460 nm).
    Z-Arg-AMC hydrochloride
  • HY-130433
    NBD Sphingosine
    		99.0%
    NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases.
    NBD Sphingosine
  • HY-D1818
    Vari Fluor 680-Phalloidin
    Vari Fluor 680-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence when labeled at 680/700 nm.
    Vari Fluor 680-Phalloidin
Cat. No. Product Name / Synonyms Application Reactivity