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  3. Cyanine5 NHS ester chloride

Cyanine5 NHS ester chloride 

Cat. No.: HY-135414
Handling Instructions

Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.

For research use only. We do not sell to patients.

Cyanine5 NHS ester chloride Chemical Structure

Cyanine5 NHS ester chloride Chemical Structure

CAS No. : 1032678-42-4

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This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Cyanine5 NHS ester chloride:

Other Forms of Cyanine5 NHS ester chloride:

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides[1].

In Vitro

Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

616.19

Formula

C36H42ClN3O4

CAS No.
SMILES

CC1(C)C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (405.72 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6229 mL 8.1144 mL 16.2288 mL
5 mM 0.3246 mL 1.6229 mL 3.2458 mL
10 mM 0.1623 mL 0.8114 mL 1.6229 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.38 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.38 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Cyanine5 NHS ester chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Cyanine5 NHS ester chloride
Cat. No.:
HY-135414
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