1. Fluorescent Dye
  2. Fluorescence Coupling Reagent
    Labeling Chemistry
    Fluorescence Wavelength Division
  3. Amine Reactive
    Cyanine
    Ex 570-590 nm Yellow
    Em 620-750 nm Red
  4. Cyanine5 NHS ester iodide

Cyanine5 NHS ester iodide 

Cat. No.: HY-135414B
COA Handling Instructions

Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.

For research use only. We do not sell to patients.

Cyanine5 NHS ester iodide Chemical Structure

Cyanine5 NHS ester iodide Chemical Structure

Size Price Stock
5 mg USD 200 Ask For Quote & Lead Time
10 mg USD 310 Ask For Quote & Lead Time
25 mg USD 550 Ask For Quote & Lead Time
50 mg USD 900 Ask For Quote & Lead Time

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Other Forms of Cyanine5 NHS ester iodide:

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides[1].

In Vitro

Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

707.64

Formula

C36H42IN3O4

Emission (Em)
Excitation (Ex)
SMILES

CC1(C)C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[I-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 110 mg/mL (155.45 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4131 mL 7.0657 mL 14.1315 mL
5 mM 0.2826 mL 1.4131 mL 2.8263 mL
10 mM 0.1413 mL 0.7066 mL 1.4131 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.75 mg/mL (3.89 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.75 mg/mL (3.89 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Cyanine5 NHS ester iodide
Cat. No.:
HY-135414B
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