Cyanine5 NHS ester iodide

Name: Cyanine5 NHS ester iodide
Brand: MedChemExpress
SKU: HY-135414B
Rating: 4.5 (1 reviews)

Cat. No.: HY-135414B Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

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Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.

For research use only. We do not sell to patients.

Cyanine5 NHS ester iodide Chemical Structure

Size	Price	Stock	Quantity
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 900	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Cyanine5 NHS ester iodide:

Cyanine5 NHS ester chloride Get quote
Cyanine5 NHS ester bromide Get quote

1 Publications Citing Use of MCE Cyanine5 NHS ester iodide

•Commun Biol. 2022 Oct 3;5(1):1052. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides^[1].

In Vitro

Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

707.64

Formula

C₃₆H₄₂IN₃O₄

Appearance

Solid

Color

Yellow to brown

SMILES

CC1(C)C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[I-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 110 mg/mL (155.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4131 mL	7.0657 mL	14.1315 mL
5 mM	0.2826 mL	1.4131 mL	2.8263 mL
10 mM	0.1413 mL	0.7066 mL	1.4131 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (3.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (3.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (259 KB) HNMR (307 KB) LCMS (242 KB)

Handling Instructions (2659 KB)

References

[1]. Brugada-Vilà P, et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics. 2020;10(6):2744-2758. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4131 mL	7.0657 mL	14.1315 mL	35.3287 mL
	5 mM	0.2826 mL	1.4131 mL	2.8263 mL	7.0657 mL
	10 mM	0.1413 mL	0.7066 mL	1.4131 mL	3.5329 mL
	15 mM	0.0942 mL	0.4710 mL	0.9421 mL	2.3552 mL
	20 mM	0.0707 mL	0.3533 mL	0.7066 mL	1.7664 mL
	25 mM	0.0565 mL	0.2826 mL	0.5653 mL	1.4131 mL
	30 mM	0.0471 mL	0.2355 mL	0.4710 mL	1.1776 mL
	40 mM	0.0353 mL	0.1766 mL	0.3533 mL	0.8832 mL
	50 mM	0.0283 mL	0.1413 mL	0.2826 mL	0.7066 mL
	60 mM	0.0236 mL	0.1178 mL	0.2355 mL	0.5888 mL
	80 mM	0.0177 mL	0.0883 mL	0.1766 mL	0.4416 mL
	100 mM	0.0141 mL	0.0707 mL	0.1413 mL	0.3533 mL