NBD-F
Based on 1 Customer Validation
NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent which is developed for amino acid analysis. NBD-F reacts with primary or secondary amines to produce a fluorescent product and used for analysis of amino acids and low molecular weight amines.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 29270-56-2
- Formula: C6H2FN3O3
- Molecular Weight:183.10
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products[1]. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 29270-56-2
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Appearance Solid
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Molecular Weight 183.10
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Formula C6H2FN3O3
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Color Light yellow to yellow
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SMILES
O=[N+](C1=CC=C(F)C2=NON=C21)[O-]
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Synonyms
4-Fluoro-7-nitrobenzofurazan
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (546.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62. [Content Brief]
[2]. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4615 mL | 27.3075 mL | 54.6150 mL | 136.5374 mL |
| 5 mM | 1.0923 mL | 5.4615 mL | 10.9230 mL | 27.3075 mL | |
| 10 mM | 0.5461 mL | 2.7307 mL | 5.4615 mL | 13.6537 mL | |
| 15 mM | 0.3641 mL | 1.8205 mL | 3.6410 mL | 9.1025 mL | |
| 20 mM | 0.2731 mL | 1.3654 mL | 2.7307 mL | 6.8269 mL | |
| 25 mM | 0.2185 mL | 1.0923 mL | 2.1846 mL | 5.4615 mL | |
| 30 mM | 0.1820 mL | 0.9102 mL | 1.8205 mL | 4.5512 mL | |
| 40 mM | 0.1365 mL | 0.6827 mL | 1.3654 mL | 3.4134 mL | |
| 50 mM | 0.1092 mL | 0.5461 mL | 1.0923 mL | 2.7307 mL | |
| 60 mM | 0.0910 mL | 0.4551 mL | 0.9102 mL | 2.2756 mL | |
| 80 mM | 0.0683 mL | 0.3413 mL | 0.6827 mL | 1.7067 mL | |
| 100 mM | 0.0546 mL | 0.2731 mL | 0.5461 mL | 1.3654 mL |
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