1. Dye Reagents
  2. NBD-F

NBD-F (Synonyms: 4-Fluoro-7-nitrobenzofurazan)

Cat. No.: HY-D0785
Handling Instructions

NBD-F is a fluorescent derivatization reagent which is originally developed for amino acid analysis.

For research use only. We do not sell to patients.

NBD-F Chemical Structure

NBD-F Chemical Structure

CAS No. : 29270-56-2

Size Price Stock Quantity
50 mg USD 140 In-stock
Estimated Time of Arrival: December 31
100 mg USD 190 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

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NBD-F is a fluorescent derivatization reagent which is originally developed for amino acid analysis.

In Vitro

As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products[1]. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable[2].


4°C, protect from light

Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (682.69 mM)

*"≥" means soluble, but saturation unknown.

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.4615 mL 27.3075 mL 54.6150 mL
5 mM 1.0923 mL 5.4615 mL 10.9230 mL
10 mM 0.5461 mL 2.7307 mL 5.4615 mL
*Please refer to the solubility information to select the appropriate solvent.
Kinase Assay

An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Cat. No.: HY-D0785