ANTS
Based on 1 Customer Validation
ANTS is a fluorescent dye (Excitation wavelength: 380 nm; Emission wavelength: 520 nm). ANTS and DPX are encapsulated in liposomes can be an effective approach for measuring membrane leakage.
For research use only. We do not sell to patients.
- Purity: 99.09%
- CAS No.: 5398-34-5
- Formula: C10H7NNa2O9S3
- Molecular Weight:427.34
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
515
350
Chemical Information
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CAS No. 5398-34-5
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Appearance Solid
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Molecular Weight 427.34
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Formula C10H7NNa2O9S3
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Color White to off-white
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SMILES
O=S(C1=CC(S(=O)(O)=O)=C2C(N)=CC(S(=O)(O[Na])=O)=CC2=C1)(O[Na])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 20 mg/mL (46.80 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Aguilera J, et al. A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins. Bio Protoc. 2021 May 20;11(10):e4025. [Content Brief]
[2]. Peng X, et al. Characterization of differential pore-forming activities of ESAT-6 proteins from Mycobacterium tuberculosis and Mycobacterium smegmatis. FEBS Lett. 2016 Feb;590(4):509-19. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3401 mL | 11.7003 mL | 23.4006 mL | 58.5014 mL |
| 5 mM | 0.4680 mL | 2.3401 mL | 4.6801 mL | 11.7003 mL | |
| 10 mM | 0.2340 mL | 1.1700 mL | 2.3401 mL | 5.8501 mL | |
| 15 mM | 0.1560 mL | 0.7800 mL | 1.5600 mL | 3.9001 mL | |
| 20 mM | 0.1170 mL | 0.5850 mL | 1.1700 mL | 2.9251 mL | |
| 25 mM | 0.0936 mL | 0.4680 mL | 0.9360 mL | 2.3401 mL | |
| 30 mM | 0.0780 mL | 0.3900 mL | 0.7800 mL | 1.9500 mL | |
| 40 mM | 0.0585 mL | 0.2925 mL | 0.5850 mL | 1.4625 mL |