KMG-104

Cat. No.: HY-139646: FAQs
Data Sheet SDS Handling Instructions

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KMG-104 is a highly selective fluorescent Mg²⁺ probe. KMG-104 has been used widely and revealed Mg²⁺ mobilization in cytoplasm in various types of cells.

For research use only. We do not sell to patients.

KMG-104 Chemical Structure

CAS No. : 852057-94-4

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Purity & Documentation
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Description

KMG-104 is a highly selective fluorescent Mg²⁺ probe. KMG-104 has been used widely and revealed Mg²⁺ mobilization in cytoplasm in various types of cells^[1].

In Vitro

KMG-104 is useful for confocal laser scanning microscopy because it is excited at 490 nm, and emitted fluorescence around 510 nm. Fluorescent intensity of KMG-104 increases with [Mg] increase, and it shows no response to Na, K, and Ca; the dissociation constant (K_d) for Mg is 3 mM. KMG-104 is able to trace only [Mg] change inphysiological conditions^[2].
PC12 cells are loaded with a novel Mg indicator KMG-104 and Ca indicator fura-2, and intracellular Mg is studied in the endoplasmic reticulums (ERs), mitochondria, and Mg-ATP^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

435.33

Formula

C₂₃H₁₁F₂NO₆

CAS No.

852057-94-4

Appearance

Solid

Color

Brown to red

SMILES

O=C(C(C(O)=O)=C1)N2C(C=CC=C2)=C1C(C3=CC(F)=C(O)C=C3O4)=C5C4=CC(C(F)=C5)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (114.86 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2971 mL	11.4855 mL	22.9711 mL
5 mM	0.4594 mL	2.2971 mL	4.5942 mL
10 mM	0.2297 mL	1.1486 mL	2.2971 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Data Sheet (280 KB) SDS (251 KB) Handling Instructions (2659 KB)

References

[1]. Kubota T, et al. Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging. J Am Coll Nutr. 2004;23(6):742S-4S. [Content Brief]

[2]. Fujii T, et al. Design and synthesis of a FlAsH-type Mg2+ fluorescent probe for specific protein labeling. J Am Chem Soc. 2014;136(6):2374-2381. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2971 mL	11.4855 mL	22.9711 mL	57.4277 mL
	5 mM	0.4594 mL	2.2971 mL	4.5942 mL	11.4855 mL
	10 mM	0.2297 mL	1.1486 mL	2.2971 mL	5.7428 mL
	15 mM	0.1531 mL	0.7657 mL	1.5314 mL	3.8285 mL
	20 mM	0.1149 mL	0.5743 mL	1.1486 mL	2.8714 mL
	25 mM	0.0919 mL	0.4594 mL	0.9188 mL	2.2971 mL
	30 mM	0.0766 mL	0.3829 mL	0.7657 mL	1.9143 mL
	40 mM	0.0574 mL	0.2871 mL	0.5743 mL	1.4357 mL
	50 mM	0.0459 mL	0.2297 mL	0.4594 mL	1.1486 mL
	60 mM	0.0383 mL	0.1914 mL	0.3829 mL	0.9571 mL
	80 mM	0.0287 mL	0.1436 mL	0.2871 mL	0.7178 mL
	100 mM	0.0230 mL	0.1149 mL	0.2297 mL	0.5743 mL

KMG-104 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KMG-104852057-94-4KMG104KMG 104Fluorescent DyefluorescentMgprobeintracellularlocalizationPC12cellsInhibitorinhibitorinhibit

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