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  4. KMG-104

KMG-104 is a cell-impermeant highly selective fluorescent Mg2+ probe. KMG-104 has been used widely and revealed Mg2+ mobilization in cytoplasm in various types of cells.

For research use only. We do not sell to patients.

KMG-104

KMG-104 Chemical Structure

CAS No. : 852057-94-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
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KMG-104 Related Antibodies

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Description

KMG-104 is a cell-impermeant highly selective fluorescent Mg2+ probe. KMG-104 has been used widely and revealed Mg2+ mobilization in cytoplasm in various types of cells[1].

In Vitro

KMG-104 can be introduced into cells by methods such as patch clamp and microinjection.
KMG-104 is suitable for confocal laser scanning microscopy because it is excited at 490 nm and emits fluorescence around 510 nm. The fluorescence intensity of KMG-104 increases with increasing Mg2+ concentration and shows no response to Na, K, or Ca2+. The dissociation constant (Kd) for magnesium is 3 mM. KMG-104 can only track changes in Mg2+ under physiological conditions[2].
The novel magnesium ion indicator KMG-104 and the calcium ion indicator fura-2 were loaded into PC12 cells to investigate intracellular magnesium ions in the endoplasmic reticulum, mitochondria, and Mg-ATP[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KMG-104 Related Antibodies
Molecular Weight

435.33

Formula

C23H11F2NO6
CAS No.
Appearance

Solid

Color

Brown to red

SMILES

O=C(C(C(O)=O)=C1)N2C(C=CC=C2)=C1C(C3=CC(F)=C(O)C=C3O4)=C5C4=CC(C(F)=C5)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (114.86 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2971 mL 11.4855 mL 22.9711 mL
5 mM 0.4594 mL 2.2971 mL 4.5942 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2971 mL 11.4855 mL 22.9711 mL 57.4277 mL
5 mM 0.4594 mL 2.2971 mL 4.5942 mL 11.4855 mL
10 mM 0.2297 mL 1.1486 mL 2.2971 mL 5.7428 mL
15 mM 0.1531 mL 0.7657 mL 1.5314 mL 3.8285 mL
20 mM 0.1149 mL 0.5743 mL 1.1486 mL 2.8714 mL
25 mM 0.0919 mL 0.4594 mL 0.9188 mL 2.2971 mL
30 mM 0.0766 mL 0.3829 mL 0.7657 mL 1.9143 mL
40 mM 0.0574 mL 0.2871 mL 0.5743 mL 1.4357 mL
50 mM 0.0459 mL 0.2297 mL 0.4594 mL 1.1486 mL
60 mM 0.0383 mL 0.1914 mL 0.3829 mL 0.9571 mL
80 mM 0.0287 mL 0.1436 mL 0.2871 mL 0.7178 mL
100 mM 0.0230 mL 0.1149 mL 0.2297 mL 0.5743 mL
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KMG-104 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KMG-104852057-94-4KMG104KMG 104Fluorescent DyefluorescentMgprobeintracellularlocalizationPC12cellsInhibitorinhibitorinhibit

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