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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-112624F
    CY7-Dextran (MW 4000)
    CY7-Dextran (MW 4000) is a fluorescent dye that consists of CY7 (HY-D0825) and Dextran (HY-112624) (Ex=740 nm; Em=770 nm). CY7-Dextran (MW 4000) can be used as a cell volume indicator and delineates the thin peripheral edges of the cells.
    CY7-Dextran (MW 4000)
  • HY-D1809
    Vari Fluor 405-Streptavidin
    Vari Fluor 405-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=405 nm/431 nm.
    Product Concentration: 0.8 mg/mL
    Vari Fluor 405-Streptavidin
  • HY-P4417A
    Ac-IEPD-AMC TFA
    		99.33%
    Ac-IEPD-AMC TFA is a fluorescent substrate used to measure protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC fluoresces under UV light irradiation and can emit fluorescent signals.
    Ac-IEPD-AMC TFA
  • HY-D0022A
    6-Aminofluorescein
    		98.84%
    6-Aminofluorescein (6-AF) is a fluorescent labeling reagent. 6-Aminofluorescein can be used to label fullerene-based liposome nanostructures termed ‘buckysomes′ (Ex/Em = 490/515 nm).
    6-Aminofluorescein
  • HY-W027773
    7-Methoxy-4-(trifluoromethyl)coumarin
    		98.0%
    7-Methoxy-4-(trifluoromethyl)coumarin (MFC) is a fluorescent substrate for cytochrome P450 and can quantify CYP activity.
    7-Methoxy-4-(trifluoromethyl)coumarin
  • HY-W001952
    6-Bromo-2-naphthol
    		99.75%
    6-Bromo-2-naphthol is an RTP (real-time polymerase chain reaction) probe that forms a 1:1 or 2:1 complex with β-cyclodextrin (β-CD). 6-Bromo-2-naphthol is capable of real-time monitoring of PCR reactions and quantification of specific nucleic acid sequences. RTP probes are a class of small DNA or RNA sequences labeled with fluorescent dyes and quencher molecules, which can be widely used in gene expression analysis, SNP genotyping, and pathogen detection. 6-Bromo-2-naphthol embeds into the cyclodextrin cavity through hydrophobic interactions, inhibits the oxygen quenching effect, and emits a phosphorescent signal at room temperature. 6-Bromo-2-naphthol can also be used as an intermediate for the synthesis of antibacterial azo dyes, and its derivatives show antibacterial activity against Staphylococcus aureus, Escherichia coli and other bacteria.
    6-Bromo-2-naphthol
  • HY-W423080
    TAMRA alkyne,5-isomer
    TAMRA alkyne, 5-isomer (Compound 3) is an alkyne derivative of TAMRA and can be used for the enrichment, in-gel fluorescence detection, and identification of O-GlcNAc-modified proteins. TAMRA alkyne, 5-isomer contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Azide groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    TAMRA alkyne,5-isomer
  • HY-W015996
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide
    		99.92%
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GalNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection.
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide
  • HY-D2339
    H-FluNox
    		99.53%
    H-FluNox is a selective fluorescent probe that senses labile heme using biomimetic N-oxide deoxygenation to trigger fluorescence. H-FluNox is more than 100-fold selective for labile heme over Fe(II), enabling the discrimination of labile heme from labile Fe(II) pools in living cells. H-FluNox is sensitive enough to detect the subcellular labile heme. (λex=490 nm, λem=535 nm)
    H-FluNox
  • HY-W923198
    Fluorescein O-methacrylate
    Fluorescein O-methacrylate (Methacryloyloxy fluorescein) is a pH-responsive fluorescent monomeric dye. Fluorescein O-methacrylate exhibits pH-responsive fluorescence properties: its fluorescence is activated under alkaline conditions and quenched under neutral or acidic conditions; reversible switching of fluorescence is achievable by adjusting the pH of the solution. Fluorescein O-methacrylate can be used as a monomer for the synthesis of amphiphilic fluorescein polymers. These polymers can self-assemble into stable polymer nanoparticles with enhanced aqueous-phase fluorescence, and also enable the conjugation of nanoparticles with amine-containing biomolecules to support sensitive optical detection of biomarkers (Ex/Em = 490/520 nm).
    Fluorescein O-methacrylate
  • HY-D2346
    HBmito Crimson
    HBmito Crimson is a deep red fluorescent probe (λex: 658 nm, λem: 678 nm) for the inner mitochondrial membrane. HBmito Crimson is a cell membrane-permeable probe with high selectivity for the mitochondrial inner membrane, suitable for specific fluorescence staining of the inner mitochondrial membrane in living cells. HBmito Crimson has high photostability and brightness, suitable for long-term dynamic fluorescence imaging.
    HBmito Crimson
  • HY-N15351
    Flavokermesic acid
    		98.18%
    Flavokermesic acid is an anthraquinone dye that can be detected in extracts of insects from the family Diaspididae, and it is a minor pigment component thereof. Flavokermesic acid can be used to investigate the biosynthetic pathways of insect-derived anthraquinone pigments.
    Flavokermesic acid
  • HY-P5354A
    FRETS-VWF73 TFA
    FRETS-VWF73 TFA, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex=340 nm; Em=450 nm).
    FRETS-VWF73 TFA
  • HY-150209
    MODAG-001
    		98.69%
    MODAG-001 can bind to synuclein fibrils in a rat brain. MODAG-001 is a candidate α-syn imaging probe.
    MODAG-001
  • HY-D1255
    Sel-green
    		99.98%
    Sel-green, a selective selenol fluorescent probe, is applied to quantify the Sec content in the selenoenzyme thioredoxin reductase and image endogenous Sec in live HepG2 cells.
    Sel-green
  • HY-DY1023
    TMRE (solution)
    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.
    Solvent and concentration: DMSO: 10 mM
    TMRE (solution)
  • HY-D1327
    Cyanine3 azide chloride
    		98.94%
    Cyanine3 azide chloride, an analog of Cy3 azide, is a potent green fluorescent dye. Cyanine3 azide chloride uses click chemistry coupled with Alkyne-labeled proteins. Cyanine3 azide chloride can be detected by fluorometers, imagers, and microscopes. (λex=684 nm, λem=710 nm). Cyanine3 azide (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cyanine3 azide chloride
  • HY-D2178
    AF 568 NHS ester
    AF 568 NHS ester is a derivative of the orange fluorescent dye AF 568. AF 568 NHS ester is widely used in cell dyes, biological dyes, biomolecules and particle fluorescent labeling. AF 568 exhibits maximum absorption wavelength of 579 nm and the maximum emission wavelength of 603 nm.
    AF 568 NHS ester
  • HY-124418
    SBI-477
    		98.0%
    SBI-477 is a chemical probe that stimulates insulin signaling by deactivating the transcription factor MondoA. SBI-477 can lead to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain-containing 4 (ARRDC4). SBI-477 coordinately inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes.
    SBI-477
  • HY-N8407
    Carminic acid
    Carminic acid is a widely used and orally active natural red pigment that can be used in industries such as food, cosmetics, and pharmaceuticals. In addition, carminic acid has anti-inflammatory and antioxidant activities.
    Carminic acid
Cat. No. Product Name / Synonyms Application Reactivity