Cyanine3 azide chloride

Cat. No.: HY-D1327 Purity: 98.94%: Data Sheet
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Cyanine3 azide chloride, an analog of Cy3 azide, is a potent green fluorescent dye. Cyanine3 azide chloride uses click chemistry coupled with Alkyne-labeled proteins. Cyanine3 azide chloride can be detected by fluorometers, imagers, and microscopes. (λ_ex=684 nm, λ_em=710 nm)^[1]. Cyanine3 azide (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

Cyanine3 azide chloride Chemical Structure

CAS No. : 1167421-28-4

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Cyanine3 azide chloride

•Adv Mater. 2025 Jun 9:e2504363. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cyanine3 azide chloride, an analog of Cy3 azide, is a potent green fluorescent dye. Cyanine3 azide chloride uses click chemistry coupled with Alkyne-labeled proteins. Cyanine3 azide chloride can be detected by fluorometers, imagers, and microscopes. (λ_ex=684 nm, λ_em=710 nm)^[1][2]>. Cyanine3 azide (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Molecular Weight

575.19

Formula

C₃₃H₄₃ClN₆O

CAS No.

1167421-28-4

Appearance

Solid

Color

Green to dark green

Emission (Em)

569

Excitation (Ex)

555

SMILES

[N-]=[N+]=NCCCNC(CCCCCN1C2=C(C(C)(C)/C1=C\C=C\C3=[N+](C)C4=C(C3(C)C)C=CC=C4)C=CC=C2)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (86.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7386 mL	8.6928 mL	17.3856 mL
5 mM	0.3477 mL	1.7386 mL	3.4771 mL
10 mM	0.1739 mL	0.8693 mL	1.7386 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.94%

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Data Sheet (274 KB) SDS (252 KB)

COA (251 KB) HNMR (313 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Kim SM, et, al. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infect Dis. 2020 Nov 13;6(11):3076-3082. [Content Brief]

[2]. Morimoto K, et, al. Triazine Probes Target Ascorbate Peroxidases in Plants. Plant Physiol. 2019 Aug;180(4):1848-1859. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7386 mL	8.6928 mL	17.3856 mL	43.4639 mL
	5 mM	0.3477 mL	1.7386 mL	3.4771 mL	8.6928 mL
	10 mM	0.1739 mL	0.8693 mL	1.7386 mL	4.3464 mL
	15 mM	0.1159 mL	0.5795 mL	1.1590 mL	2.8976 mL
	20 mM	0.0869 mL	0.4346 mL	0.8693 mL	2.1732 mL
	25 mM	0.0695 mL	0.3477 mL	0.6954 mL	1.7386 mL
	30 mM	0.0580 mL	0.2898 mL	0.5795 mL	1.4488 mL
	40 mM	0.0435 mL	0.2173 mL	0.4346 mL	1.0866 mL
	50 mM	0.0348 mL	0.1739 mL	0.3477 mL	0.8693 mL
	60 mM	0.0290 mL	0.1449 mL	0.2898 mL	0.7244 mL
	80 mM	0.0217 mL	0.1087 mL	0.2173 mL	0.5433 mL