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  4. TAMRA azide, 5-isomer

TAMRA azide, 5-isomer is a click chemical containing azide groups that can be used as a linker for TAMRA. The azide group of TAMRA Azide, 5-isomer enables copper-catalyzed click chemical reactions with alkynes, DBCO, and BCN. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups (Ex/Em = 555/580 nm).

For research use only. We do not sell to patients.

CAS No. : 825651-66-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

TAMRA azide, 5-isomer Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE TAMRA azide, 5-isomer

Cell Imaging/Staining

    TAMRA azide, 5-isomer purchased from MedChemExpress. Usage Cited in: iScience. 2025 May 2;28(6):112569.  [Abstract]

    EdU incorporation was conducted at E14.5 for 1 h to see proliferating cells in S-phase (EdU) (4 control brains including 2 Atf6afl/flAtf6bfl/fl embryos and 2 Atf6afl/flAtf6bfl/+ embryos, and 4 dcKO brains). TAMRA azide, 5-isomer (5-TAMRA-Azide, 0.5 μM), was utilized as a dye in this experiment.

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    Description

    TAMRA azide, 5-isomer is a click chemical containing azide groups that can be used as a linker for TAMRA. The azide group of TAMRA Azide, 5-isomer enables copper-catalyzed click chemical reactions with alkynes, DBCO, and BCN[1][2]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups (Ex/Em = 555/580 nm).

    In Vitro

    Click chemistry is used to describe the selective, modular, wide range and high-yield chemical reactions that allow rapid synthesis of new compounds through heteroatom linking (C-X-C). Bertozzi developed click chemistry in a new dimension —Bioorthogonal chemistry. It is defined as a rapid and selective reaction that does not interfere with biological processes under physiological conditions. Common reaction structures of click chemistry, such as Azide, Alkyne, DBCO, BCN, TCO and Tetrazine.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TAMRA azide, 5-isomer Related Antibodies
    Molecular Weight

    512.56

    Formula

    C28H28N6O4
    CAS No.
    Appearance

    Solid

    Color

    Brown to reddish brown

    SMILES

    [N-]=[N+]=NCCCNC(C1=CC(C([O-])=O)=C(C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)C=C1)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (243.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9510 mL 9.7550 mL 19.5099 mL
    5 mM 0.3902 mL 1.9510 mL 3.9020 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9510 mL 9.7550 mL 19.5099 mL 48.7748 mL
    5 mM 0.3902 mL 1.9510 mL 3.9020 mL 9.7550 mL
    10 mM 0.1951 mL 0.9755 mL 1.9510 mL 4.8775 mL
    15 mM 0.1301 mL 0.6503 mL 1.3007 mL 3.2517 mL
    20 mM 0.0975 mL 0.4877 mL 0.9755 mL 2.4387 mL
    25 mM 0.0780 mL 0.3902 mL 0.7804 mL 1.9510 mL
    30 mM 0.0650 mL 0.3252 mL 0.6503 mL 1.6258 mL
    40 mM 0.0488 mL 0.2439 mL 0.4877 mL 1.2194 mL
    50 mM 0.0390 mL 0.1951 mL 0.3902 mL 0.9755 mL
    60 mM 0.0325 mL 0.1626 mL 0.3252 mL 0.8129 mL
    80 mM 0.0244 mL 0.1219 mL 0.2439 mL 0.6097 mL
    100 mM 0.0195 mL 0.0975 mL 0.1951 mL 0.4877 mL
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    TAMRA azide, 5-isomer Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TAMRA azide, 5-isomer825651-66-9Fluorescent Dyeclick chemistryhigh yieldhigh specificitysimplicitybindingInhibitorinhibitorinhibit

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