Tris(3-hydroxypropyltriazolylmethyl)amine
Based on 3 publication(s) in Google Scholar
Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) is an accelerating ligand in the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) and protects cells from oxidants generated by copper-catalyzed reduction of oxygen by ascorbate. In addition, Tris(3-hydroxypropyltriazolylmethyl)amine can also protect the histidine moiety of biomolecules in a manner proportional to the ligand concentration.
For research use only. We do not sell to patients.
- Purity: 98.98%
- CAS No.: 760952-88-3
- Formula: C18H30N10O3
- Molecular Weight:434.50
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tris(3-hydroxypropyltriazolylmethyl)amine
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Biological Activity
Tris(3-hydroxypropyltriazolylmethyl)amine (1.25 mM, 1 h) intercepts and quickly reduce reactive oxygen species generated by the ascorbate-driven reduction of dissolved O2 without compromising the CuAAC reaction rate very much[1].
Tris(3-hydroxypropyltriazolylmethyl)amine (250 μM, 1-5 min) reverses the toxic effect of Cu-ascorbate effect in a dose-dependent manner at a 5:1 ligand:Cu ratio, preserving the viability of HeLa, Chinese hamster ovary (CHO), and Jurkat cells[2].
Tris(3-hydroxypropyltriazolylmethyl)amine (125 μM, 5 min) accelerates the click reaction and allows the cells to survive at a copper concentration of 50 μM in HeLa cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:125 μM
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Incubation Time:5 min
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Result:Resulted the HeLa cells alive up to the copper concentration of 50 μM.
Chemical Information
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CAS No. 760952-88-3
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Appearance Solid
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Molecular Weight 434.50
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Formula C18H30N10O3
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Color White to light brown
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SMILES
OCCCN1N=NC(CN(CC2=CN(CCCO)N=N2)CC3=CN(CCCO)N=N3)=C1
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Synonyms
THPTA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Nature
2025 Nov;647(8089):506-516. PMID: 40931066 -
Adv Mater
Cell Surface-Confined DNAzyme Coordination-Facilitated Logical Engineering for Precise Cell Identification. [Abstract]2025 Aug;37(34):e2504363. PMID: 40489061 -
Phytomedicine
Delphinidin targets voltage-dependent anion channel 1 to inhibit ferroptosis and protect against retinal photochemical damage. [Abstract]2026 Jun 17:159:158455. PMID: 42335641
Solvent & Solubility
H2O : ≥ 175 mg/mL (402.76 mM)
DMSO : 100 mg/mL (230.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Hong V, et al. Analysis and optimization of copper-catalyzed azide-alkyne cycloaddition for bioconjugation. Angew Chem Int Ed Engl. 2009;48(52):9879-83. [Content Brief]
[2]. Hong V, et al. Labeling live cells by copper-catalyzed alkyne− azide click chemistry. Bioconjugate chemistry 21.10 (2010): 1912-1916. [Content Brief]
[3]. Rathinam S, et al. Conjugation of CRAMP18-35 Peptide to Chitosan and Hydroxypropyl Chitosan via Copper-Catalyzed Azide-Alkyne Cycloaddition and Investigation of Antibacterial Activity. Int J Mol Sci. 2024 Aug 30;25(17):9440. [Content Brief]
[4]. Yuan Y, et al. Bioorthogonal Turn-On Probe Based on Aggregation-Induced Emission Characteristics for Cancer Cell Imaging and Ablation. Angew Chem Int Ed Engl. 2016 May 23;55(22):6457-61. [Content Brief]
[5]. Kozarski M, et al. Towards superior mRNA caps accessible by click chemistry: synthesis and translational properties of triazole-bearing oligonucleotide cap analogs. RSC Adv. 2023 Apr 25;13(19):12809-12824. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.3015 mL | 11.5075 mL | 23.0150 mL | 57.5374 mL |
| 5 mM | 0.4603 mL | 2.3015 mL | 4.6030 mL | 11.5075 mL | |
| 10 mM | 0.2301 mL | 1.1507 mL | 2.3015 mL | 5.7537 mL | |
| 15 mM | 0.1534 mL | 0.7672 mL | 1.5343 mL | 3.8358 mL | |
| 20 mM | 0.1151 mL | 0.5754 mL | 1.1507 mL | 2.8769 mL | |
| 25 mM | 0.0921 mL | 0.4603 mL | 0.9206 mL | 2.3015 mL | |
| 30 mM | 0.0767 mL | 0.3836 mL | 0.7672 mL | 1.9179 mL | |
| 40 mM | 0.0575 mL | 0.2877 mL | 0.5754 mL | 1.4384 mL | |
| 50 mM | 0.0460 mL | 0.2301 mL | 0.4603 mL | 1.1507 mL | |
| 60 mM | 0.0384 mL | 0.1918 mL | 0.3836 mL | 0.9590 mL | |
| 80 mM | 0.0288 mL | 0.1438 mL | 0.2877 mL | 0.7192 mL | |
| 100 mM | 0.0230 mL | 0.1151 mL | 0.2301 mL | 0.5754 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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