Alkynyl Palmitic Acid (Synonyms: Alk-C16)

Name: Alkynyl Palmitic Acid
Brand: MedChemExpress
SKU: HY-W040304
Rating: 5.0 (3 reviews)

Cat. No.: HY-W040304 Purity: 99.90%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Alkynyl Palmitic Acid (Alk-C16) is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. Alkynyl Palmitic Acid is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

CAS No. : 99208-90-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Alkynyl Palmitic Acid

: Alkynyl Palmitic Acid purchased from MedChemExpress. Usage Cited in: J Virol. 2025 May 22:e0034725. [Abstract]; HEK-293T cells expressing PEDV S-Flag were labeled with Alkynyl Palmitic Acid (Alkynyl PA) (50 μM; 8 h), then subjected to IP, click chemistry (± HAM), streptavidin pull-down, and IB analysis.

: Alkynyl Palmitic Acid purchased from MedChemExpress. Usage Cited in: J Virol. 2025 May 22:e0034725. [Abstract]; HEK-293T cells expressing PEDV S-Flag were treated with 40 µM 2-BP for 24 h, labeled with Alkynyl Palmitic Acid (Alkynyl PA) (50 μM; 8 h), then subjected to IP, click chemistry, streptavidin pull-down, and IB analysis.

: Alkynyl Palmitic Acid purchased from MedChemExpress. Usage Cited in: J Virol. 2025 May 22:e0034725. [Abstract]; Vero cells were infected with PEDV (MOI=0.05) for 12 h, then metabolically labeled with Alkynyl Palmitic Acid (Alkynyl PA) (50 μM; 8 h). Cell lysates were immunoprecipitated with anti-PEDV S mAb and protein G beads, subjected to click chemistry (± HAM), eluted with glycine-HCl, pulled down with streptavidin beads, and analyzed by IB.

: Alkynyl Palmitic Acid purchased from MedChemExpress. Usage Cited in: J Virol. 2025 May 22:e0034725. [Abstract]; Vero cells were infected with PEDV (MOI=0.05) for 12 h, treated with or without 6 µM 2-BP, then metabolically labeled with Alkynyl Palmitic Acid (Alkynyl PA) (50 μM; 8 h), subjected to IP, click chemistry, streptavidin pull-down, and IB analysis.

: Alkynyl Palmitic Acid purchased from MedChemExpress. Usage Cited in: J Virol. 2025 May 22:e0034725. [Abstract]; Vero cells were infected with PEDV (MOI=0.05) for 12 h, then metabolically labeled with Alkynyl Palmitic Acid (Alkynyl PA) (50 μM; 8 h). Mature virions were purified from the supernatant by sucrose gradient ultracentrifugation, then subjected to IP, click chemistry (± HAM), streptavidin pull-down, and IB analysis.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All PROTAC Linkers Isoform Specific Products:

View All Isoforms

PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers

Biological Activity
Purity & Documentation
References
Customer Review

Description

Alkynyl Palmitic Acid (Alk-C16) is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs^[1]. Alkynyl Palmitic Acid is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

Alkyl-Chain

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

252.39

Formula

C₁₆H₂₈O₂

CAS No.

99208-90-9

Appearance

Solid

Color

White to off-white

SMILES

C#CCCCCCCCCCCCCCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (396.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (198.11 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9621 mL	19.8106 mL	39.6212 mL
5 mM	0.7924 mL	3.9621 mL	7.9242 mL
10 mM	0.3962 mL	1.9811 mL	3.9621 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.91 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.91 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (267 KB) SDS (396 KB)

COA (239 KB) RP-HPLC (243 KB)

Handling Instructions (2659 KB)

References

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.9621 mL	19.8106 mL	39.6212 mL	99.0531 mL
	5 mM	0.7924 mL	3.9621 mL	7.9242 mL	19.8106 mL
	10 mM	0.3962 mL	1.9811 mL	3.9621 mL	9.9053 mL
	15 mM	0.2641 mL	1.3207 mL	2.6414 mL	6.6035 mL
	20 mM	0.1981 mL	0.9905 mL	1.9811 mL	4.9527 mL
	25 mM	0.1585 mL	0.7924 mL	1.5848 mL	3.9621 mL
	30 mM	0.1321 mL	0.6604 mL	1.3207 mL	3.3018 mL
	40 mM	0.0991 mL	0.4953 mL	0.9905 mL	2.4763 mL
	50 mM	0.0792 mL	0.3962 mL	0.7924 mL	1.9811 mL
	60 mM	0.0660 mL	0.3302 mL	0.6604 mL	1.6509 mL
	80 mM	0.0495 mL	0.2476 mL	0.4953 mL	1.2382 mL
	100 mM	0.0396 mL	0.1981 mL	0.3962 mL	0.9905 mL

Alkynyl Palmitic Acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Alkynyl Palmitic Acid99208-90-9Alk-C16PROTAC LinkersInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Alkynyl Palmitic Acid (Synonyms: Alk-C16)

Customer Review

Alkynyl Palmitic Acid Related Antibodies

3 Publications Citing Use of MCE Alkynyl Palmitic Acid

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All PROTAC Linkers Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Alkynyl Palmitic Acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Alkynyl Palmitic Acid Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report