Fluorescein O-methacrylate (Synonyms: Methacryloyloxy fluorescein)

Cat. No.: HY-W923198 Purity: 95.80%: Data Sheet
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Fluorescein O-methacrylate (Methacryloyloxy fluorescein) is a pH-responsive fluorescent monomeric dye. Fluorescein O-methacrylate exhibits pH-responsive fluorescence properties: its fluorescence is activated under alkaline conditions and quenched under neutral or acidic conditions; reversible switching of fluorescence is achievable by adjusting the pH of the solution. Fluorescein O-methacrylate can be used as a monomer for the synthesis of amphiphilic fluorescein polymers. These polymers can self-assemble into stable polymer nanoparticles with enhanced aqueous-phase fluorescence, and also enable the conjugation of nanoparticles with amine-containing biomolecules to support sensitive optical detection of biomarkers (Ex/Em = 490/520 nm).

For research use only. We do not sell to patients.

Fluorescein O-methacrylate Chemical Structure

CAS No. : 480439-15-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Description

In Vitro

Fluorescein O-methacrylate (0.25-0.9 mol%; 25 h, 3 cycles) incorporated into PBPEM₄₁₅-g-(PBA-co-PFMA)₄₀ bottlebrushes confers pH-responsive fluorescence, with no emission at pH 6 or 7 and strong green emission at pH 8, 9, or 10, and this switching behavior is fully reversible across three cycles with minimal quenching^[1].
Fluorescein O-methacrylate (0.2 mmol; 24 h polymerization) -containing amphiphilic copolymers (poly(FMA-r-NAS-r-MA)) self-assemble into stable, size-tunable spherical nanoparticles with enhanced fluorescence, and FPNP1 (incorporating 8% Fluorescein O-methacrylate (Methacryloyloxy fluorescein)) exhibits optimal dispersion stability and BSA immobilization efficiency, making it a suitable platform for optical biosensors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

400.38

Formula

C₂₄H₁₆O₆

CAS No.

480439-15-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C(C)=C)OC1=CC=C2C(OC3=CC(O)=CC=C3C24OC(C5=C4C=CC=C5)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (249.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4976 mL	12.4881 mL	24.9763 mL
5 mM	0.4995 mL	2.4976 mL	4.9953 mL
10 mM	0.2498 mL	1.2488 mL	2.4976 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.80%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (252 KB) HNMR (257 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Alper Nese, et al. pH-Responsive Fluorescent Molecular Bottlebrushes Prepared by Atom Transfer Radical Polymerization. Macromolecules 2011, 44, 15, 5905-5910.

[2]. Woo J, et al. Novel fluorescein polymer-based nanoparticles: facile and controllable one-pot synthesis, assembly, and immobilization of biomolecules for application in a highly sensitive biosensor. RSC Adv. 2020;10(5):2998-3004. Published 2020 Jan 16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4976 mL	12.4881 mL	24.9763 mL	62.4407 mL
	5 mM	0.4995 mL	2.4976 mL	4.9953 mL	12.4881 mL
	10 mM	0.2498 mL	1.2488 mL	2.4976 mL	6.2441 mL
	15 mM	0.1665 mL	0.8325 mL	1.6651 mL	4.1627 mL
	20 mM	0.1249 mL	0.6244 mL	1.2488 mL	3.1220 mL
	25 mM	0.0999 mL	0.4995 mL	0.9991 mL	2.4976 mL
	30 mM	0.0833 mL	0.4163 mL	0.8325 mL	2.0814 mL
	40 mM	0.0624 mL	0.3122 mL	0.6244 mL	1.5610 mL
	50 mM	0.0500 mL	0.2498 mL	0.4995 mL	1.2488 mL
	60 mM	0.0416 mL	0.2081 mL	0.4163 mL	1.0407 mL
	80 mM	0.0312 mL	0.1561 mL	0.3122 mL	0.7805 mL
	100 mM	0.0250 mL	0.1249 mL	0.2498 mL	0.6244 mL

Fluorescein O-methacrylate Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescein O-methacrylate480439-15-4Methacryloyloxy fluoresceinFluorescent Dyemolecular imagingaqueous fluorescenceoptical biosensorsbiomarker optical detectionamphiphilic fluorescein polymerspH-responsive fluorescencebottlebrush polymerspolymeric nanoparticlesamine-containing biomoleculesconfocal microscopyInhibitorinhibitorinhibit

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Fluorescein O-methacrylate (Synonyms: Methacryloyloxy fluorescein)

Fluorescein O-methacrylate Related Antibodies

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Fluorescein O-methacrylate Related Antibodies

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