HBmito Crimson

HBmito Crimson (10 μM; 24 h) shows > 95% HeLa cell viability with low phototoxicity^[1].

HBmito Crimson (500 nM; 15 min) imaging of COS7 cells during apoptosis shows cristae widening and IM herniation accompanied by mtDNA leakage^[1].

HBmito Crimson (500 nM) STED imaging in COS7 cells shows that ferroptosis reduces class III mitochondria to < 18% and increases the mtDNA/mitochondrial area ratio to 0.363^[1].

HBmito Crimson super-resolution imaging shows that in LPS-induced A549 cells, L-Clausenamide (50 μM; 24 h) attenuates mitochondrial morphological deterioration, whereas an AKT inhibitor diminishes this rescue effect^[7].

HBmito Crimson (500 nM; 10 min) enables MC-ISM to resolve mitochondrial cristae and dynamic changes within 1000 frames at 7.5 fps in live U2OS cells without obvious mitochondrial swelling^[3].
HBmito Crimson (1-5 μM; overnight before imaging) enables MC-ISM of the plant mitochondrial inner membrane in Arabidopsis hypocotyls with a lateral resolution of 136 nm^[3].
HBmito Crimson (400 nM; 10-15 min) reports a rapid Vnf-associated fluorescence increase following FCCP (HY-100410)-induced depolarization in isolated cardiac mitochondria, with a Nernstian dissipation time constant of 10.6 s^[4].
HBmito Crimson (50-1000 nM; 10-15 min) localizes to the cristae of the inner mitochondrial membrane in live HeLa/COS-7/U2OS cells, with Pearson coefficients of 0.91 (MitoTracker Green) and 0.89 (PK Mito Orange), and 400 nM is the optimal concentration for voltage imaging^[4].
HBmito Crimson (400 nM; 10-15 min) reports spontaneous action potentials in intact HeLa cells and isolated cardiac mitochondria, with an average amplitude of 0.55 ΔF/F₀ and a fast kinetic component accounting for 0.79 of the signal^[4].
HBmito Crimson (400 nM; 10-15 min) resolves miniature mitochondrial Vnf-related flickers in isolated mitochondria and intact cells, with a mean FDHM of 82.3 ms and a peak amplitude of approximately 0.03 ΔF/F₀^[4].
HBmito Crimson (0.4 µM; 30 min) colocalizes with mitochondrial PDI-TPP in HeLa cells, with a Pearson coefficient of 0.87 under basal conditions, 0.86 after CCCP (HY-100941)-induced depolarization, and 0.88 after fixation^[8].

HBmito Crimson (15 min) does not directly respond to antimycin A-induced ROS burst in isolated respiring mitochondria^[4].

HBmito Crimson (2 h; 10 min) exhibits high photostability in lipid environments and PMMA films^[1].
HBmito Crimson (5 μM) has a low saturation power of 0.864 mW in DMSO^[1].
HBmito Crimson specifically labels mitochondria in cultured cells, with colocalization coefficients of 0.91 and 0.89^[1].
HBmito Crimson (500 nM; 15 min) enables wash-free live-cell STED imaging, imaging COS7 mitochondria at 40 nm resolution and performing > 500-frame time-lapse imaging within 52 min^[1].
Dual-color imaging of HBmito Crimson (500 nM; 15 min)/SYBR Gold in COS7 cells shows that mtDNA preferentially localizes to mitochondrial tips and branch points, with a probability of mtDNA at branch points of 0.677, and that the mtDNA area ratio in small mitochondria is higher than that in networked mitochondria^[1].
HBmito Crimson (750 nM; 15 min) labels mitochondria in live COS-7 cells and enables two-color STED imaging with mScarlet3-H-labeled ER, resolving tight ER-mitochondria contacts and coordinated movement within 400 s^[2].
HBmito Crimson fluorescent dye samples show ≤ 0.3% spot spacing variation within a 100 μm FOV under MC-ISM^[3].
HBmito Crimson (500 nM; 10 min) enables 16.7 fps imaging in live COS7 cells using 25 raw frames, where FISTA-GS reconstruction outperforms jRL^[3].
HBmito Crimson (2.0 mM-10 µM) is a near-infrared Si-rhodamine probe with absorption/emission maxima at 660/688 nm in cell-free lipid solutions, exhibiting high brightness and high photostability^[4].
HBmito Crimson (400 nM; 10-15 min) localizes to the cell surface of Escherichia coli DH5α protoplasts and reports spontaneous action potentials with an average amplitude of 0.52 ΔF/F₀^[4].
HBmito Crimson (500 nM; incubated at 37°C for 15 min) labels live COS-7 cells for long-term time-lapse confocal imaging^[5].