2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-DY1035
    Hydroxyphenyl Fluorescein (solution)
    Hydroxyphenyl Fluorescein (HPF) (solution) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm.
    Solvent and concentration: DMSO: 10 mM
    Hydroxyphenyl Fluorescein (solution)
  • HY-DY2001
    Crystal Violet (solution)
    Crystal Violet (solution) , also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining.
    Solvent and Concentration: Sterile water: 5 mg/mL (0.5%)
    Crystal Violet (solution)
  • HY-D1865
    Cy3 dimethyl iodide
    		99.42%
    Cy3 dimethyl iodide is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) with a dimethyl group in the iodide salt form. The iodide salt form increases the water solubility of the compound, making it suitable for use in aqueous solutions. Cy3 is a near-infrared fluorescent dye commonly used in biolabeling and cell imaging. Cy3 dimethyl iodide binds to biomolecules such as proteins and antibodies to track their location and dynamic changes in biological samples.
    Cy3 dimethyl iodide
  • HY-NP0138
    Peanut Agglutinin (Fluorescein)
    Peanut Agglutinin (PNA) Fluorescein is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Peanut Agglutinin (PNA) Fluorescein is a biological material or organic compound that can be used in life science research.
    Peanut Agglutinin (Fluorescein)
  • HY-DY1028
    Fluo-4 AM (solution)
    Fluo-4 AM (solution) is a cell-permeable Ca2+ (calcium ion) indicator (Ex/Em = 485/526 nm) .
    Solvent and concentration: DMSO: 1 mM
    Fluo-4 AM (solution)
  • HY-D0202
    Arsenazo III
    Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca2+, Mg2+, Sr2+, Ba2+, K+, and Na+, and its binding affinity for Ca2+ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca2+ in cells.
    Arsenazo III
  • HY-145445
    BIOTIN-PEG11-SH
    		98.0%
    BIOTIN-PEG11-SH enables biotin labeling of protein.
    BIOTIN-PEG11-SH
  • HY-110257
    BDY FL-X, SE
    		99.62%
    BODIPY FL-X,SE is a fluorescent greendye for the labeling of amine, shows a high fluorescence quantum yield and is relatively insensitive to pH change. BODIPY FL-X,SE can be used as an alternative for FAM, Cy2 or FITC. λabs: 504 nm; λem:510 nm.
    BDY FL-X, SE
  • HY-D1256
    Msr-blue
    		99.38%
    Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm).
    Msr-blue
  • HY-D1090
    ​JOE
    JOE is a xanthene fluorophore (i.e., 4′,5′-dichloro-2′,7′-dimethoxy-5 (6)-carboxyfluorescein; 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein) with an absorption wavelength of approximately 525 nm and an emission wavelength of approximately 550 nm. The fluorescence quantum yield of JOE correlates with the rigidity of the linker arm and the distance to dG nucleoside. JOE is commonly used as a fluorescent label for oligonucleotides and molecular beacon probes, and also serves as the acceptor fluorophore in fluorescence energy transfer primers for DNA sequencing.
    ​JOE
  • HY-W035051
    TSPP tetrasodium
    TSPP tetrasodium is a photosensitizer that has shown impressive effects in in vivo regression of cancer and microorganism infections (Ex: 413 nm, Em: 640 nm).
    TSPP tetrasodium
  • HY-D1832
    Vari Fluor 532 TSA (200×)
    Vari Fluor 532 TSA (200×) (VF 532 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 532 TSA (200×)
  • HY-131010
    Flutax-2 (5/6-mixture)
    Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers.
    Flutax-2 (5/6-mixture)
  • HY-139407
    3-C12-NBD-cholesterol
    		99.9%
    3-C12-NBD-cholesterol is a fluorescent cholesterol analog that can be used for measuring membrane and intracellular trafficking dynamics.
    3-C12-NBD-cholesterol
  • HY-130022
    HKPerox-1
    		99.11%
    HKPerox-1 is a highly sensitive green fluorescent probe for the specific detection of H2O2 in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.
    HKPerox-1
  • HY-D1732
    FITC-hyodeoxycholic acid
    		98.02%
    FITC-hyodeoxycholic acid is a hyodeoxycholic acid labeled with FITC, which can be used to study the mechanism of action of hyodeoxycholic acid.
    FITC-hyodeoxycholic acid
  • HY-D1321A
    Cy 5 amine TFA
    		99.83%
    Cy 5 amine (Cyanine5 amine) TFA is a fluorescent dye. Cy 5 amine TFA can be used in the preparation of Cy5.5-labeled compound or polymers, which can be used for imaging cellular process and trafficking.
    Cy 5 amine TFA
  • HY-D0110A
    Fura-2 pentapotassium
    Fura-2 pentapotassium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca2+ level.
    Fura-2 pentapotassium
  • HY-W127769
    Rose-β-D-Gal
    		99.71%
    Rose-β-D-Gal is a chromogenic substrate, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity.
    Rose-β-D-Gal
  • HY-W075603
    2,6-Diiodo-BODIPY 493/503
    2,6-Diiodo-BODIPY 493/503 is an F-Bodipy fluorescent dye with diiodo substitution at the (pyrrole) 2,6 position. 2,6-Diiodo-BODIPY 493/503 has a pyrrole iodine substituent that turns it red.
    2,6-Diiodo-BODIPY 493/503
Cat. No. Product Name / Synonyms Application Reactivity