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  3. BDY FL-X, SE

BODIPY FL-X,SE is a fluorescent greendye for the labeling of amine, shows a high fluorescence quantum yield and is relatively insensitive to pH change. BODIPY FL-X,SE can be used as an alternative for FAM, Cy2 or FITC. λabs: 504 nm; λem:510 nm.

For research use only. We do not sell to patients.

BDY FL-X, SE Chemical Structure

BDY FL-X, SE Chemical Structure

CAS No. : 217190-09-5

Size Price Stock Quantity
1 mg USD 140 In-stock
5 mg USD 300 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

BODIPY FL-X,SE is a fluorescent greendye for the labeling of amine, shows a high fluorescence quantum yield and is relatively insensitive to pH change. BODIPY FL-X,SE can be used as an alternative for FAM, Cy2 or FITC. λabs: 504 nm; λem:510 nm[1].

In Vitro

BODIPY FL-X, succinimidyl ester dye stock solutions are prepared in DMSO. Then, it is diluted in 0.1 M sodium bicarbonate buffer, pH 8.3, to a final concentration of 10 μM dye.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

502.32

Formula

C24H29BF2N4O5

CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

515

Excitation (Ex)

505

SMILES

O=C(CCC1=O)N1OC(CCCCCNC(CCC2=CC=C(C=C3C(C)=C4)[N-]2[B+3]([F-])([F-])[N]3=C4C)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (165.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9908 mL 9.9538 mL 19.9076 mL
5 mM 0.3982 mL 1.9908 mL 3.9815 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9908 mL 9.9538 mL 19.9076 mL 49.7691 mL
5 mM 0.3982 mL 1.9908 mL 3.9815 mL 9.9538 mL
10 mM 0.1991 mL 0.9954 mL 1.9908 mL 4.9769 mL
15 mM 0.1327 mL 0.6636 mL 1.3272 mL 3.3179 mL
20 mM 0.0995 mL 0.4977 mL 0.9954 mL 2.4885 mL
25 mM 0.0796 mL 0.3982 mL 0.7963 mL 1.9908 mL
30 mM 0.0664 mL 0.3318 mL 0.6636 mL 1.6590 mL
40 mM 0.0498 mL 0.2488 mL 0.4977 mL 1.2442 mL
50 mM 0.0398 mL 0.1991 mL 0.3982 mL 0.9954 mL
60 mM 0.0332 mL 0.1659 mL 0.3318 mL 0.8295 mL
80 mM 0.0249 mL 0.1244 mL 0.2488 mL 0.6221 mL
100 mM 0.0199 mL 0.0995 mL 0.1991 mL 0.4977 mL
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BDY FL-X, SE Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BDY FL-X, SE
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