BDY FL-X, SE
Based on 1 Customer Validation
BODIPY FL-X,SE is a fluorescent greendye for the labeling of amine, shows a high fluorescence quantum yield and is relatively insensitive to pH change. BODIPY FL-X,SE can be used as an alternative for FAM, Cy2 or FITC. λabs: 504 nm; λem:510 nm.
For research use only. We do not sell to patients.
- Purity: 98.20%
- CAS No.: 217190-09-5
- Formula: C24H29BF2N4O5
- Molecular Weight:502.32
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
BDY FL-X, SE, succinimidyl ester dye stock solutions are prepared in DMSO. Then, it is diluted in 0.1 M sodium bicarbonate buffer, pH 8.3, to a final concentration of 10 μM dye.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
505
Chemical Information
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CAS No. 217190-09-5
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Appearance Solid
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Molecular Weight 502.32
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Formula C24H29BF2N4O5
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Color Yellow to green
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SMILES
O=C(CCC1=O)N1OC(CCCCCNC(CCC2=CC=C(C=C3C(C)=C4)[N-]2[B+3]([F-])([F-])[N]3=C4C)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 83.33 mg/mL (165.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. K P Top, et al.Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes. Electrophoresis. 2001 Mar;22(5):896-905. [Content Brief]
[2]. Karen Martin, et al. Simultaneous red/green dual fluorescence detection on electroblots using BODIPY TR-X succinimidyl ester and ELF 39 phosphate.Proteomics. 2002 May;2(5):499-512. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9908 mL | 9.9538 mL | 19.9076 mL | 49.7691 mL |
| 5 mM | 0.3982 mL | 1.9908 mL | 3.9815 mL | 9.9538 mL | |
| 10 mM | 0.1991 mL | 0.9954 mL | 1.9908 mL | 4.9769 mL | |
| 15 mM | 0.1327 mL | 0.6636 mL | 1.3272 mL | 3.3179 mL | |
| 20 mM | 0.0995 mL | 0.4977 mL | 0.9954 mL | 2.4885 mL | |
| 25 mM | 0.0796 mL | 0.3982 mL | 0.7963 mL | 1.9908 mL | |
| 30 mM | 0.0664 mL | 0.3318 mL | 0.6636 mL | 1.6590 mL | |
| 40 mM | 0.0498 mL | 0.2488 mL | 0.4977 mL | 1.2442 mL | |
| 50 mM | 0.0398 mL | 0.1991 mL | 0.3982 mL | 0.9954 mL | |
| 60 mM | 0.0332 mL | 0.1659 mL | 0.3318 mL | 0.8295 mL | |
| 80 mM | 0.0249 mL | 0.1244 mL | 0.2488 mL | 0.6221 mL | |
| 100 mM | 0.0199 mL | 0.0995 mL | 0.1991 mL | 0.4977 mL |
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