Peanut Agglutinin (Fluorescein)
Based on 1 Customer Validation
Peanut Agglutinin (PNA) Fluorescein is a fluorescently labeled lectin that specifically binds to glycosylated cells and Haemonchus contortus. Peanut Agglutinin Fluorescein specifically recognizes and binds to the D-Gal-β(1→3)-D-GalNAc disaccharide sequence and terminal D-galactosyl residues on the cell surface; its binding can be inhibited by D-galactose but is not affected by glucose. Peanut Agglutinin Fluorescein specifically labels cone photoreceptor segments and can also be used to distinguish immature thymocytes from lymphocyte subsets, serving as an effective tool for studying cellular glycosylation status and isolating specific cell populations.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Fluorescein-conjugated Peanut Agglutinin (FITC-PNA) (100-200 μg/mL; 15-30 min) binds specifically to cone photoreceptor inner and outer segments in human, monkey, rabbit, mouse, chicken, and fish retinas, with additional species-specific binding to cone-associated synaptic structures, and this binding is galactose-dependent[1].
Fluorescein-conjugated Peanut Agglutinin (FITC-PNA) (200 μg/mL; 30 min) binds specifically to cone outer segment tips and cone inner/outer segment plasma membranes in whole-mount rabbit retinas, with no binding to rod photoreceptors[1].
Peanut Agglutinin Fluorescein (0.5 mg/mL, 5 μg; 30 min) stains 80%-90% of isolated thymocytes and 2.7%-15.8% of isolated peripheral lymphocytes; the proportion of labeled cells is higher among dense adult CBA/J thymocytes than among low-density thymocytes. Furthermore, this reagent detects cells at the early stages of lymphopoiesis in isolated fetal cells, serving as a marker for immature or early T-cell subpopulations[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Peanut Agglutinin Fluorescein (5 mg/mL; 1 h) maintains specific binding to Haemonchus contortus eggs fixed with 0.5-5% formalin and stored at 4°C for no more than 4 weeks, while a decrease in staining consistency is observed after 5 weeks of storage[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Color Light yellow to yellow
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SMILES
[Peanut Agglutinin (Fluorescein)]
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Synonyms
PNA (Fluorescein)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[2]. London J, Berrih S, Bach JF. Peanut agglutinin. I. A new tool for studying T lymphocyte subpopulations. Journal of immunology (Baltimore, Md. : 1950). 1978 Aug;121(2):438-43. [Content Brief]
[3]. Jurasek ME, Bishop-Stewart JK, Storey BE, et al.. Modification and further evaluation of a fluorescein-labeled peanut agglutinin test for identification of Haemonchus contortus eggs. Veterinary parasitology. 2010 Apr 19;169(1-2):209-13. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Peanut Agglutinin (Fluorescein) Related Classifications
HY-NP0138 Related Classifications
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- Peanut Agglutinin (Fluorescein)
- PNA (Fluorescein)
- Fluorescent Dye
- Parasite
- mouse thymocytes
- peripheral lymphocytes
- Haemonchus contortus eggs
- trichostrongyle eggs
- bone marrow cells
- D-Gal-β(1→3) D-GalNAc
- cone photoreceptor segments
- terminal D-galactosyl residues
- galactose-galactosamine disaccharide residues
- sialic acid
- Inhibitor
- inhibitor
- inhibit