Flutax-2 (5/6-mixture)
Based on 1 Customer Validation
Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers.
For research use only. We do not sell to patients.
- Purity: 88.82%
- Formula: C142H128F4N4O42
- Molecular Weight:1319.27
-
Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be adjusted according to your specific needs).
Preparation of Flutax-2 working solution
1.1 Preparation of stock solution
Prepare a 10 mM stock solution in DMSO.
1.2 Preparation of working solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a Flutax-2 working solution at 100 nM-1 μM.
Note: Adjust the concentration of Flutax-2 working solution according to actual needs and prepare fresh before use.
2. Cell staining
2.1 Suspension cells: Collect cells by centrifugation and wash twice with PBS, 5 min each time.
Adherent cells: Remove the culture medium, add trypsin to digest the cells. After centrifugation, discard the supernatant and wash twice with PBS, 5 min each time.
2.2 Add 1 mL Flutax-2 working solution and incubate at room temperature for 10–30 min.
2.3 Centrifuge at 400 g, 4°C for 3-4 min and discard the supernatant.
2.4 Wash cells twice with PBS, 5 min each time.
2.5 Resuspend cells in 1 mL serum-free medium or PBS and observe using a fluorescence microscope.
Storage conditions
Store at -20°C protected from light for one year.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
520
495
Chemical Information
-
Appearance Solid
-
Molecular Weight 1319.27
-
Formula C142H128F4N4O42
-
Color Light yellow to yellow
-
SMILES
O=C1OC2(C3=CC=C(C(N[C@H](C(O[C@H]([C@](C([C@H](OC(C)=O)C4=C5C)=O)(C)[C@@]6([C@H](OC(C7=CC=CC=C7)=O)[C@@](C[C@@H]5OC([C@@H]([C@@H](NC(C8=CC=CC=C8)=O)C9=CC=CC=C9)O)=O)(O)C4(C)C)[H])C[C@@]%10([H])OC[C@]%106OC(C)=O)=O)C)=O)C=C31)C%11=CC(F)=C(O)C=C%11OC%12=CC(O)=C(F)C=C2%12.O=C%13OC%14(C%15=CC(C(N[C@H](C(O[C@H]([C@](C([C@H](OC(C)=O)C%16=C%17C)=O)(C)[C@@]%18([C@H](OC(C%19=CC=CC=C%19)=O)[C@@](C[C@@H]%17OC([C@@H]([C@@H](NC(C%20=CC=CC=C%20)=O)C%21=CC=CC=C%21)O)=O)(O)C%16(C)C)[H])C[C@@]%22([H])OC[C@]%22%18OC(C)=O)=O)C)=O)=CC=C%15%13)C%23=CC(F)=C(O)C=C%23OC%24=CC(O)=C(F)C=C%14%24
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 62.5 mg/mL (47.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (291 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91. [Content Brief]
[2]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7580 mL | 3.7900 mL | 7.5799 mL | 18.9499 mL |
| 5 mM | 0.1516 mL | 0.7580 mL | 1.5160 mL | 3.7900 mL | |
| 10 mM | 0.0758 mL | 0.3790 mL | 0.7580 mL | 1.8950 mL | |
| 15 mM | 0.0505 mL | 0.2527 mL | 0.5053 mL | 1.2633 mL | |
| 20 mM | 0.0379 mL | 0.1895 mL | 0.3790 mL | 0.9475 mL | |
| 25 mM | 0.0303 mL | 0.1516 mL | 0.3032 mL | 0.7580 mL | |
| 30 mM | 0.0253 mL | 0.1263 mL | 0.2527 mL | 0.6317 mL | |
| 40 mM | 0.0189 mL | 0.0947 mL | 0.1895 mL | 0.4737 mL |
Powered by Bioz