Timosaponin AIII

Name: Timosaponin AIII
Brand: MedChemExpress
SKU: HY-N0810
Rating: 5.0 (2 reviews)

製品番号: HY-N0810 純度: 99.86%: Data Sheet SDS COA 取扱説明書
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Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC₅₀ of 35.4 μM.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Timosaponin AIII 構造式

CAS 番号 : 41059-79-4

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 130	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 130	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$38	在庫あり	Estimated Time of Arrival: December 31
5 mg	$80	在庫あり	Estimated Time of Arrival: December 31
10 mg	$140	在庫あり	Estimated Time of Arrival: December 31
25 mg	$290	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of Timosaponin AIII:

Timosaponin AIII (Standard) 在庫あり

顧客検証

Cholinesterase (ChE) アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

AChE BChE

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC₅₀ of 35.4 μM.

IC₅₀ & Target

AChE

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	5.5 μM Compound: 1	Growth inhibition of human HCT116 cells Growth inhibition of human HCT116 cells	[PMID: 21370894]
HCT-15	IC₅₀	6.1 μM Compound: 1	Cytotoxicity against human HCT15 cells after 3 days Cytotoxicity against human HCT15 cells after 3 days	[PMID: 21370894]
HCT-15	IC₅₀	6.1 μM Compound: 1	Growth inhibition of human HCT15 cells Growth inhibition of human HCT15 cells	[PMID: 21370894]
Hs68	IC₅₀	>50 μM Compound: 1	Growth inhibition of human HS68 cells Growth inhibition of human HS68 cells	[PMID: 21370894]
HT-29	IC₅₀	10.3 μM Compound: 1	Growth inhibition of human HT-29 cells Growth inhibition of human HT-29 cells	[PMID: 21370894]
SW480	IC₅₀	13.1 μM Compound: 1	Growth inhibition of human SW480 cells Growth inhibition of human SW480 cells	[PMID: 21370894]
SW-620	IC₅₀	11.1 μM Compound: 1	Growth inhibition of human SW620 cells Growth inhibition of human SW620 cells	[PMID: 21370894]

体外実験

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC₅₀ of 35.4 μM^[1]. Timosaponin AIII is identified as a major selective cytotoxic activity in BN108, and its selective cytotoxic activity involves inhibition of mTOR, induction of ER stress and protective autophagy^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Of the tested steroidal saponins, Timosaponin AIII (TA3) most potently improves memory deficits. Timosaponin AIII increases the scopolamine-induced reduction in step-through latency by 17% (10 mg/kg), 28% (20 mg/kg), and 43% (40 mg/kg). During the acquisition trial, no differences in latent time are observed. Timosaponin AIII (20, 40 mg/kg, p.o.) potently inhibits this reduction of acetylcholine in scopolamine-treated mouse brain. The inhibitory effect of Timosaponin AIII is comparable to that of tacrine, which is used as a positive control^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

740.92

分子式

C₃₉H₆₄O₁₃

CAS 番号

41059-79-4

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@H](C)CO4)([H])C[C@@]1([H])[C@@](CC[C@@]5([H])[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@H](O)[C@@H]7O)([H])[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)C5)C)([H])[C@]6([H])CC2

Structure Classification

Steroids

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 125 mg/mL (168.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3497 mL	6.7484 mL	13.4967 mL
5 mM	0.2699 mL	1.3497 mL	2.6993 mL
10 mM	0.1350 mL	0.6748 mL	1.3497 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.86%

Select Batch:

データシート (278 KB) SDS (393 KB)

COA (254 KB) HNMR (243 KB) RP-HPLC (236 KB) MS (176 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Lee B, et al. Timosaponin AIII, a saponin isolated from Anemarrhena asphodeloides, ameliorates learning and memory deficits in mice. Pharmacol Biochem Behav. 2009 Aug;93(2):121-7. [Content Brief]

[2]. King FW, et al. Timosaponin AIII is preferentially cytotoxic to tumor cells through inhibition of mTOR and induction of ER stress. PLoS One. 2009 Sep 30;4(9):e7283. [Content Brief]

動物実験
^[1]

Mice^[1]
Male ICR mice weighing 28-30 g are used. For the acquisition trial, mice are initially placed in the illuminated compartment and the door between the two compartments is opened 10 s later. Each group contains ten mice. One hour or 5 h before the acquisition trial, mice receive each test agent (e.g., Timosaponin AIII 10, 20 or 40 mg/kg, p.o. ). One hour before the acquisition trial, mice receive tacrine (10 mg/kg, p.o.) as a positive control. Memory impairment is induced by scopolamine treatment (1 mg/kg, i.p.) 0.5 h or 4.5 h after the administration of test agents, tacrine, or 10% Tween 80 solution. Control animals are administered 10% Tween 80 solution alone. Twenty-four hours after the acquisition trial, the mice are again placed in the illuminated compartment for retention trials. The time taken for a mouse to enter the dark compartment after the door opened is measured as the latency time in both acquisition and retention trials, with a maximum of 300 s^[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Lee B, et al. Timosaponin AIII, a saponin isolated from Anemarrhena asphodeloides, ameliorates learning and memory deficits in mice. Pharmacol Biochem Behav. 2009 Aug;93(2):121-7. [Content Brief]

[2]. King FW, et al. Timosaponin AIII is preferentially cytotoxic to tumor cells through inhibition of mTOR and induction of ER stress. PLoS One. 2009 Sep 30;4(9):e7283. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3497 mL	6.7484 mL	13.4967 mL	33.7418 mL
	5 mM	0.2699 mL	1.3497 mL	2.6993 mL	6.7484 mL
	10 mM	0.1350 mL	0.6748 mL	1.3497 mL	3.3742 mL
	15 mM	0.0900 mL	0.4499 mL	0.8998 mL	2.2495 mL
	20 mM	0.0675 mL	0.3374 mL	0.6748 mL	1.6871 mL
	25 mM	0.0540 mL	0.2699 mL	0.5399 mL	1.3497 mL
	30 mM	0.0450 mL	0.2249 mL	0.4499 mL	1.1247 mL
	40 mM	0.0337 mL	0.1687 mL	0.3374 mL	0.8435 mL
	50 mM	0.0270 mL	0.1350 mL	0.2699 mL	0.6748 mL
	60 mM	0.0225 mL	0.1125 mL	0.2249 mL	0.5624 mL
	80 mM	0.0169 mL	0.0844 mL	0.1687 mL	0.4218 mL
	100 mM	0.0135 mL	0.0675 mL	0.1350 mL	0.3374 mL