2. Membrane Transporter/Ion Channel
  3. Na+/K+ ATPase
  4. Tolbutamide

トルブタミド  (Synonyms: Tolbutamide)

製品番号: HY-B0401 純度: 99.69%
COA 取扱説明書 Technical Support

Tolbutamide is an orally active KATP inhibitor. Tolbutamide inhibits cell proliferation, stimulates exocytosis of glucagon and reduces fetal lethality of mice. Tolbutamide can be used in the research of diabete.

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CAS 番号 : 64-77-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
500 mg $50 在庫あり
1 g $72 在庫あり
5 g $108 在庫あり
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カスタマーレビュー

Based on 10 publication(s) in Google Scholar

Other Forms of Tolbutamide:

トルブタミド 関連抗体

Top Publications Citing Use of Products

    Tolbutamide purchased from MedChemExpress. Usage Cited in: Egypt J Neurosurg. 2026 Jan 8;41(1):4.

    Perforated cell recording of GT1-7 cells incubated in bath solution supplemented 2.5 mM glucose before and after perfusion of vehicle (top trace) or 10 nM leptin (bottom trace). After the membrane potential stabilized, 300 µM tolbutamide (tolb) was added to block KATP currents.

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    製品説明

    Tolbutamide is an orally active KATP inhibitor. Tolbutamide inhibits cell proliferation, stimulates exocytosis of glucagon and reduces fetal lethality of mice. Tolbutamide can be used in the research of diabete[1][2][3][4].

    Cellular Effect
    Cell Line Type Value Description References
    Sf21 IC50
    >1 2
    Compound: Tolbutamide
    Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
    Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
    		[PMID: 21965623]
    Sf21 IC50
    > 1000 3
    Compound: Tolbutamide
    Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
    Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
    		[PMID: 21965623]
    Sf21 IC50
    > 1000 3
    Compound: Tolbutamide
    Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
    Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
    		[PMID: 21965623]
    体外実験

    Tolbutamide (400 μM, 24h) with dbcAMP reduces glioma cell proliferation by increasing connexin43 (Cx43)[1].
    Tolbutamide (0.1 μM, 1 min) stimulates exocytosis by activation of a mitochondrial­like KATP channel in rat A-cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    トルブタミド 関連抗体

    Western Blot Analysis[1]

    Cell Line: Glioma cell
    Concentration: 400 μM
    Incubation Time: 24h
    Result: Increasing the level of Cx43.
    体内実験

    Tolbutamide (125 mg/kg, Supplemented in daily diet for 27 weeks) reduces the incidence of diabetes mellitus in the non-obese-diabetic mouse[3].
    Tolbutamide (100-400 mg/kg, Intraperitoneal injection, 400 mg/kg on day 13; 100 mg/kg on day 10-13; combined treatment: 100 mg/kg on day 10-12 and 400 mg/kg on day 13) reduces fetal lethality in pregnant mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Non-obese-diabetic mouse [3]
    Dosage: 125 mg/kg
    Administration: Supplemented in daily diet for 27 weeks
    Result: Showed 10 of 23 animals developed diabetes compared with 18 of 24 in the control groups.
    Animal Model: Pregnant mice [4]
    Dosage: 100 mg/kg, 400 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Showed a significant reduction (50 %) in the frequency of living fetuses for the group of 400 mg/kg on day 13.
    Had significantly fewer petechiae and more late resorptions for the group of 400 mg/kg on day 13.
    分子量

    270.35

    分子式

    C12H18N2O3S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=S(C1=CC=C(C)C=C1)(NC(NCCCC)=O)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 34 mg/mL (125.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6989 mL 18.4945 mL 36.9891 mL
    5 mM 0.7398 mL 3.6989 mL 7.3978 mL
    10 mM 0.3699 mL 1.8495 mL 3.6989 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.69 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.69 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.96%

    COA (245 KB) HNMR (201 KB) RP-HPLC (173 KB) LCMS (121 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6989 mL 18.4945 mL 36.9891 mL 92.4727 mL
    5 mM 0.7398 mL 3.6989 mL 7.3978 mL 18.4945 mL
    10 mM 0.3699 mL 1.8495 mL 3.6989 mL 9.2473 mL
    15 mM 0.2466 mL 1.2330 mL 2.4659 mL 6.1648 mL
    20 mM 0.1849 mL 0.9247 mL 1.8495 mL 4.6236 mL
    25 mM 0.1480 mL 0.7398 mL 1.4796 mL 3.6989 mL
    30 mM 0.1233 mL 0.6165 mL 1.2330 mL 3.0824 mL
    40 mM 0.0925 mL 0.4624 mL 0.9247 mL 2.3118 mL
    50 mM 0.0740 mL 0.3699 mL 0.7398 mL 1.8495 mL
    60 mM 0.0616 mL 0.3082 mL 0.6165 mL 1.5412 mL
    80 mM 0.0462 mL 0.2312 mL 0.4624 mL 1.1559 mL
    100 mM 0.0370 mL 0.1849 mL 0.3699 mL 0.9247 mL
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    Tolbutamide Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tolbutamide64-77-7Na+/K+ ATPaseSodium potassium pumpInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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