Acetoacetyl-CoA  (Synonyms: 3-Oxobutanoyl-coenzyme A)

Acetoacetyl CoA is the precursor of HMG-CoA in the mevalonate pathway. Acetoacetyl-CoA thiolase catalyzes the reaction to form acetoacetyl-CoA from two acetyl-CoA molecules. Acetoacetyl CoA is essential for cholesterol biosynthesis. Acetoacetyl-CoA is also a intermediate in the biological breakdown and synthesis of fatty acids^[1][2][3].

The purified recombinant Msed_0389 and Msed_1423 catalyze the NAD-dependent oxidation of (S)-3-hydroxybutyryl-CoA to Acetoacetyl-CoA and are highly specific for their substrate (K_m of 2.6 μM and 5 μM, respectively)^[2].
The homologous bifunctional protein is the only enzyme capable to covert crotonyl-CoA into Acetoacetyl-CoA in autotrophic Desulfurococcales (Ignicoccus hospitalis) and Thermoproteales (Pyrobaculum neutrophilus) that use the dicarboxylate/4-hydroxybutyrate cycle for CO2 fixation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

851.61

C₂₅H₄₀N₇O₁₈P₃S

1420-36-6

O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(OP(OCC(C)(C)[C@@H](O)C(NCCC(NCCSC(CC(C)=O)=O)=O)=O)(O)=O)(O)=O)O[C@H]1N2C=NC3=C(N)N=CN=C32

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Miziorko HM. Enzymes of the mevalonate pathway of isoprenoid biosynthesis. Arch Biochem Biophys. 2011 Jan 15;505(2):131-43.  [Content Brief]

  [2]. Liu L, et al. Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula. Front Microbiol. 2020 Mar 11;11:354.  [Content Brief]

  [3]. Feodor Lynen, et al. Enzymes of fatty acid metabolism. Biochimica et Biophysica Acta. Volume 12, Issues 1-2, October 1953, Pages 299-314.