Amizon  (Synonyms: Enisamium iodide)

Amizon is an orally effective antiviral and anti-inflammatory agent. Amizon inhibits influenza virus replication and restricts viral RNA synthesis. Amizon reduces the mRNA expression of COX-1, COX-2, NF-κB, TGF1β, IL-1 and IL-6, and stimulates the secretion and mRNA expression of IL-10. Amizon inhibits the oxidative activity of macrophages and possesses antioxidant and free radical-scavenging activities. Amizon is applicable to research related to influenza and acute respiratory viral infections^[1][2].

Enisamium (40-1000 μM; 24 h) dose-dependently reduces replication of multiple influenza A (including oseltamivir-resistant influenza A^H275Y mutant) and B viruses in differentiated NHBE cells, with the greatest reduction of 2.8 log₁₀TCID₅₀/mL observed at 1000 μM for 24 hours against influenza A/TN/1-560/09 [H1N1]^[1].
Enisamium (500-1000 μM; 24-48 h) significantly reduces replication of influenza A/Brisbane/59/2007 (H1N1) virus in differentiated NHBE cells at MOIs of 0.01, 0.001, and 0.0001 PFU/cell when administered post-infection^[1].
Enisamium (10-1000 μM; 24 h) exhibits low permeability (1.1-1.6%) across differentiated NHBE cells after 24 hours of exposure at 10-1000 μM, with intracellular concentrations increasing in a dose-dependent manner^[1].
Enisamium (2000 μM; total 24 h) significantly reduces replication and matrix gene expression of influenza A/Brisbane/59/2007 (H1N1) virus in differentiated NHBE cells when added between 1 hour before and 10 hours after viral inoculation for a total 24-hour incubation^[1].
Enisamium (100-1000 μM; 24 h pre-incubation, maintained throughout) significantly reduces replication of influenza A/Brisbane/59/2007 (H1N1) virus in differentiated NHBE cells over 24 and 48 hours post-inoculation^[1].
Enisamium iodide (10-100 μM; 48 h) inhibits mRNA expression of pro-inflammatory mediators (COX-1, COX-2, NF-κB, TGF1β, IL-1, IL-6) and increases anti-inflammatory IL-10 mRNA expression in phytohemagglutinin-stimulated human PBMC, with 10 μM reducing target pro-inflammatory gene expression by up to 67% and increasing IL-10 expression by 73%^[2].
Enisamium iodide (50 μM; 72 h) stimulates secretion of the anti-inflammatory cytokine IL-10 by 1.5-fold with statistical significance in phytohemagglutinin-stimulated human PBMC^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

354.19

C₁₄H₁₅IN₂O

201349-37-3

Solid

Light yellow to yellow

C[N+]1=CC=C(C(NCC2=CC=CC=C2)=O)C=C1.[I-]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. David Boltz, et al. Antiviral Activity of Enisamium Against Influenza Viruses
  in Differentiated Normal Human Bronchial Epithelial Cells.

  [2]. Kareva EN,et al. The mechanisms of anti-inflammatory action of enisamium iodide. Ter Arkh. 2022 Dec 26;94(11):1262-1267. Russian.