Anti-Mouse CXCR4 Antibody (Cx4Mab-1) 

Anti-Mouse CXCR4 Antibody is a monoclonal antibody that specifically recognizes murine CXCR4 (C-X-C chemokine receptor 4), also known as fusin or CD184. CXCR4 is a seven-transmembrane G protein–coupled receptor whose principal endogenous ligand is CXCL12 (stromal cell–derived factor-1α, SDF-1α) and is widely expressed in hematopoietic cells, endothelial cells, neurons, as well as embryonic and adult stem cells. The CXCR4–CXCL12 signaling axis activates multiple downstream pathways, including ERK1/2, Ras, p38 MAPK, PLC/MAPK, and SAPK/JNK, thereby regulating cell survival, proliferation, migration, and stemness maintenance. Aberrant overexpression of CXCR4 is closely associated with poor prognosis and metastasis in various cancers, with CXCR4-positive tumor cells preferentially home to CXCL12-rich tissues such as the liver, bone marrow, lung, and lymph nodes. Accordingly, CXCR4 and its CXCL12-related antagonists emerge as attractive targets for experimental anticancer therapy. Anti-Mouse CXCR4 Antibody is generated using a cell-based immunization and screening strategy and exhibits high affinity for both endogenous and exogenous murine CXCR4. Anti-Mouse CXCR4 Antibody can be used for thestudy of chronic lymphocytic leukemia and multiple myeloma^[1].

Anti-Mouse CXCR4 Antibody (0.01-10 μg/mL, 30 min) bound to CHO/mCXCR4 cells (overexpressing mCXCR4) and P3U1 cells (endogenously expressing mCXCR4) in a dose-dependent manner, with fluorescence intensity increasing with concentration, but it did not bind to CHO-K1 cells^[1].
Anti-Mouse CXCR4 Antibody exhibits a K_D of 6.4 × 10^-9 M for CHO/mCXCR4 cells and a K_D of 2.3 × 10^-9 M for P3U1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[Anti-Mouse CXCR4 Antibody (Cx4Mab-1)]

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ouchida T, et al. Cx4Mab-1: A Novel Anti-Mouse CXCR4 Monoclonal Antibody for Flow Cytometry. Monoclon Antib Immunodiagn Immunother. 2024 Feb;43(1):10-16.  [Content Brief]