2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. CU-CPT 4a

CU-CPT 4a  (Synonyms: TLR3-IN-1)

製品番号: HY-108473 純度: 99.50%
COA 取扱説明書 Technical Support

CU-CPT 4a (TLR3-IN-1) is a potent, highly selective TLR3 signaling inhibitor. CU-CPT 4a represses the expression of downstream signaling pathways mediated by the TLR3/dsRNA complex, including TNF-α and IL-1β.

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CAS 番号 : 1279713-77-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 157 在庫あり
Solution
10 mM * 1 mL in DMSO USD 157 在庫あり
Solid
5 mg $143 在庫あり
10 mg $242 在庫あり
25 mg $484 在庫あり
50 mg $678 在庫あり
100 mg $950 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 12 publication(s) in Google Scholar

Other Forms of CU-CPT 4a:

CU-CPT 4a 関連抗体

Top Publications Citing Use of Products

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 May;14(5):e70083.  [Abstract]

    m6A levels in EVs regulate inflammatory responses via TLR8 in macrophages. Differentiated THP‐1 cells (dTHP‐1) were pretreated with or without CU‐CPT‐9a (HY-112667; 48 h) and CU‐CPT‐4a (HY-108473; 48 h). Whole‐cell lysates obtained from dTHP‐1 cells were subjected to Western blot analysis using an anti‐phospho p65 antibody, anti‐p65 antibody and anti‐β‐actin antibody. Representative images from three independent experiments are shown.

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 May;14(5):e70083.  [Abstract]

    m6A levels in EVs regulate inflammatory responses via TLR8 in macrophages. Differentiated THP‐1 cells (dTHP‐1) were pretreated with or without CU‐CPT‐9a (HY-112667; 48 h) and CU‐CPT‐4a (HY-108473; 48 h). Conditioned medium from dTHP‐1 cells treated with CCD‐841‐CoN or HT29 small EVs was used for ELISA. dTHP‐1 cells were pre‐transfected with or without TLR8 siRNA.

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: Mol Pain. 2025 May 23:17448069251346373.  [Abstract]

    TLR3 antagonist CU CPT4a inhibited NTG-induced mechanical allodynia. Repeated treatment with CU CPT4a (5 mg/kg, i.p.) or vehicle (equal volumes of DMSO) every other day before NTG injections for 9 days. Repeated treatment with CU CPT4a inhibited basal hind paw mechanical and periorbital mechanical allodynia.

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: Mol Pain. 2025 May 23:17448069251346373.  [Abstract]

    TLR3 antagonist CU CPT4a inhibited NTG-induced mechanical allodynia. Repeated treatment with CU CPT4a (5 mg/kg, i.p.) or vehicle (equal volumes of DMSO) every other day before NTG injections for 9 days. Post-treatment responses, including hind paw mechanical and periorbital mechanical allodynia, were estimated 2 h after NTG administration.

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: Mol Pain. 2025 May 23:17448069251346373.  [Abstract]

    TLR3 antagonist CU CPT4a (5 mg/kg; i.p.; every other day for 9 days before NTG injection) reduced the expression of TLR3 in TNC. Quantitative analysis of the number of TLR3+Neuron in the TNC in a 212 × 212 µm² visual field of each section per mouse after NTG injection.

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: Mol Pain. 2025 May 23:17448069251346373.  [Abstract]

    TLR3 antagonist CU CPT4a (5 mg/kg; i.p.; every other day for 9 days before NTG injection) reduced the expression of TLR3 in TNC. Immunoblot analysis of TLR3 (n = 5).

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Nov 3;14(1):7048.  [Abstract]

    Immunofluorescence images of MMP21 in the absence or presence of RIG012 or CU-CPT 4a. β-cat ΔN/RasV12 cells were mixed with β-cat ΔN cells in the presence of BFA, and were treated with DMSO, 2.5 μM RIG012 or 50 μM CU-CPT 4a. Cells were stained with Hoechst 33342 (blue) and anti-MMP21 antibody (white).

    CU-CPT 4a purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Nov 3;14(1):7048.  [Abstract]

    Immunofluorescence images of MMP21 or p65 in intestinal organoids from APCMin-Villin-RasV12 mice in the absence or presence of 10 μM RIG012 or 100 μM CU-CPT 4a.

    Toll-like Receptor (TLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    CU-CPT 4a (TLR3-IN-1) is a potent, highly selective TLR3 signaling inhibitor. CU-CPT 4a represses the expression of downstream signaling pathways mediated by the TLR3/dsRNA complex, including TNF-α and IL-1β[1].

    IC50 & Target[1]

    TLR3

    3.44 μM (IC50, in RAW 264.7 cells)

    体外実験

    CU-CPT 4a shows dose-dependent inhibitory effects blocking Poly (I:C)-induced TLR3 activation with an IC50 of 3.44 μM[1].
    CU-CPT 4a competes with dsRNA for binding to TLR3 with a Ki of 2.96 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CU-CPT 4a 関連抗体
    分子量

    377.82

    分子式

    C18H13ClFNO3S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)[C@@H](CC1=CC=CC=C1)NC(C2=C(Cl)C3=CC=C(F)C=C3S2)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (264.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6468 mL 13.2338 mL 26.4676 mL
    5 mM 0.5294 mL 2.6468 mL 5.2935 mL
    10 mM 0.2647 mL 1.3234 mL 2.6468 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション
    参考文献
    細胞実験
    [1]

    RAW 264.7 cells are planted in 6-well plates in duplicate at 1,000,000 cells per well with 3 mL of medium (RPMI 1640 medium, supplemented with 10% FBS, Penicillin (100 U/mL) and Streptomycin (100 mg/mL)) and grown for 24 h at 37°C in a 5% CO2 humidified incubator. After 24 h, non-adherent cells and media are removed and replaced with fresh RPMI 1640 medium (3 mL/well). Two wells of adherent macrophages are treated with Poly (I:C) (15 μg/mL). One of the two wells is treated with 27 μM CU CPT 4a. One additional well is treated with only CU CPT 4a (27 μM) only. Plates are then incubated for an additional 24 h. After removal of the medium, cells are washed with PBS (3×1 mL), the 6 well plate is put on ice, then 500 μL of lysis buffer is added in each well (Lysis Buffer: 120 μL 0.5M EDTA; 12 mL Mammalian Protein Extraction Reagent, 100 μL cocktail, 0.36 mL NaCl (5 M, aqueous) ). After 5 min, the mixture is transferred into corresponding 1.5 mL tube, spun for 20 min at 13.2 K rpm in a cold room. Approximately 400 μL of supernatant are collected into new tubes, frozen at -80°C until ready for cytokine measurement[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6468 mL 13.2338 mL 26.4676 mL 66.1691 mL
    5 mM 0.5294 mL 2.6468 mL 5.2935 mL 13.2338 mL
    10 mM 0.2647 mL 1.3234 mL 2.6468 mL 6.6169 mL
    15 mM 0.1765 mL 0.8823 mL 1.7645 mL 4.4113 mL
    20 mM 0.1323 mL 0.6617 mL 1.3234 mL 3.3085 mL
    25 mM 0.1059 mL 0.5294 mL 1.0587 mL 2.6468 mL
    30 mM 0.0882 mL 0.4411 mL 0.8823 mL 2.2056 mL
    40 mM 0.0662 mL 0.3308 mL 0.6617 mL 1.6542 mL
    50 mM 0.0529 mL 0.2647 mL 0.5294 mL 1.3234 mL
    60 mM 0.0441 mL 0.2206 mL 0.4411 mL 1.1028 mL
    80 mM 0.0331 mL 0.1654 mL 0.3308 mL 0.8271 mL
    100 mM 0.0265 mL 0.1323 mL 0.2647 mL 0.6617 mL
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    CU-CPT 4a Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CU-CPT 4a1279713-77-7TLR3-IN-1Toll-like Receptor (TLR)TLR3/dsRNA complexTNF-αIL-1βprotein-RNA interactionInhibitorinhibitorinhibit

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    製品名:
    CU-CPT 4a
    製品番号:
    HY-108473
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