2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP disodium

Cyclic-di-GMP disodium  (Synonyms: c-di-GMP disodium; cyclic diguanylate disodium; 5GP-5GP disodium)

製品番号: HY-110382 純度: 98.78%
COA 取扱説明書 Technical Support

Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research.

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CAS 番号 : 2222132-40-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 727 在庫あり
Solution
10 mM * 1 mL in Water USD 727 在庫あり
Solid
1 mg $170 在庫あり
5 mg $450 在庫あり
10 mg $620 在庫あり
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カスタマーレビュー

Based on 9 publication(s) in Google Scholar

Other Forms of Cyclic-di-GMP disodium:

Cyclic-di-GMP disodium 関連抗体

Top Publications Citing Use of Products

    Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) increased the protein expression of GSDMD-N, cleaved caspase-3, and phosphorylated-IRF3 (p-IRF3) in C57BL/6 N mice (Pan02-luc cells).

    Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) indicated the good biocompatibility in C57BL/6 N mice (Pan02-luc cells) by H&E staining of the major organs.

    Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) showed antitumour effect in C57BL/6 N mice (Pan02-luc cells).

    Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    agarose gel electrophoresis analysis of DNA degradation assays. Nuclease activity assays were performed by incubating 0.5 μM VcCap5 with 20 ng/μl DNA in the presence of 0.5 μM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

    Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    second messenger-dependent activation of VcCap5 DNA cleavage. Nuclease activity assays were performed by incubating 1 µM VcCap5 with 10 ng/µl DNA in the presence of 1 µM or 5 µM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

    Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    dose-response analysis of VcCap5 activity with increasing concentrations of cyclic dinucleotides. The nuclease assay mixture contained 1 µM VcCap5, 10 ng/µl DNA, and cyclic dinucleotide (Cyclic-di-GMP disodium; c-di-GMP) over a concentration range of 0.1 to 15 µM.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

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    • 純度とドキュメンテーション

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    製品説明

    Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research[1][2][3][4].

    IC50 & Target
    体外実験

    Cyclic-di-GMP disodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
    Cyclic-di-GMP disodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
    Cyclic-di-GMP disodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cyclic-di-GMP disodium 関連抗体

    Cell Proliferation Assay[1]

    Cell Line: H508 cells
    Concentration: 0.5-50 µM
    Incubation Time: 5 days
    Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

    Cell Viability Assay[2]

    Cell Line: Jurkat cells
    Concentration: 50 µM
    Incubation Time: 24 h
    Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

    Cell Cycle Analysis[2]

    Cell Line: Jurkat cells
    Concentration: 50 µM
    Incubation Time: 24 h
    Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
    体内実験

    Cyclic-di-GMP disodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
    Dosage: 100 µg/per
    Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
    Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
    Enhanced the anti-tumor activity of TriVax.
    分子量

    734.37

    分子式

    C20H22N10Na2O14P2
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)(O[Na])=O)[C@H]3COP7(O[Na])=O
    Structure Classification
    Initial Source
    輸送条件

    Shipping with dry ice.
    保管条件

    -80°C, protect from light, stored under nitrogen
    溶剤 & 溶解度
    体外: 

    H2O : 160 mg/mL (217.87 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3617 mL 6.8086 mL 13.6171 mL
    5 mM 0.2723 mL 1.3617 mL 2.7234 mL
    10 mM 0.1362 mL 0.6809 mL 1.3617 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (68.09 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.3617 mL 6.8086 mL 13.6171 mL 34.0428 mL
    5 mM 0.2723 mL 1.3617 mL 2.7234 mL 6.8086 mL
    10 mM 0.1362 mL 0.6809 mL 1.3617 mL 3.4043 mL
    15 mM 0.0908 mL 0.4539 mL 0.9078 mL 2.2695 mL
    20 mM 0.0681 mL 0.3404 mL 0.6809 mL 1.7021 mL
    25 mM 0.0545 mL 0.2723 mL 0.5447 mL 1.3617 mL
    30 mM 0.0454 mL 0.2270 mL 0.4539 mL 1.1348 mL
    40 mM 0.0340 mL 0.1702 mL 0.3404 mL 0.8511 mL
    50 mM 0.0272 mL 0.1362 mL 0.2723 mL 0.6809 mL
    60 mM 0.0227 mL 0.1135 mL 0.2270 mL 0.5674 mL
    80 mM 0.0170 mL 0.0851 mL 0.1702 mL 0.4255 mL
    100 mM 0.0136 mL 0.0681 mL 0.1362 mL 0.3404 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Cyclic-di-GMP disodium Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cyclic-di-GMP disodium2222132-40-1c-di-GMP disodium cyclic diguanylate disodium 5GP-5GP disodiumSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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