2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP diammonium

Cyclic-di-GMP diammonium  (Synonyms: c-di-GMP diammonium; cyclic diguanylate diammonium; 5GP-5GP diammonium)

Cat. No.: HY-107780B Purity: 99.72%
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Cyclic-di-GMP diammonium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP diammonium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP diammonium can be used in cancer research.

For research use only. We do not sell to patients.

Cyclic-di-GMP diammonium Chemical Structure

Cyclic-di-GMP diammonium Chemical Structure

CAS No. : 609343-82-0

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1 mg USD 170 In-stock
5 mg USD 450 In-stock
10 mg USD 620 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of Cyclic-di-GMP diammonium:

Cyclic-di-GMP diammonium Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Cyclic-di-GMP diammonium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP diammonium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP diammonium can be used in cancer research[1][2][3][4].

IC50 & Target
In Vitro

Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclic-di-GMP diammonium Related Antibodies

Cell Proliferation Assay[1]

Cell Line: H508 cells
Concentration: 0.5-50 µM
Incubation Time: 5 days
Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
In Vivo

Cyclic-di-GMP diammonium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
Dosage: 100 µg/per
Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax.
Molecular Weight

724.47

Formula

C20H30N12O14P2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP([O-])(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)=O)[C@H]3COP7([O-])=O.[NH4+].[NH4+]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (69.02 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3803 mL 6.9016 mL 13.8032 mL
5 mM 0.2761 mL 1.3803 mL 2.7606 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (138.03 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.72%

COA (255 KB) HNMR (199 KB) RP-HPLC (244 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.3803 mL 6.9016 mL 13.8032 mL 34.5080 mL
5 mM 0.2761 mL 1.3803 mL 2.7606 mL 6.9016 mL
10 mM 0.1380 mL 0.6902 mL 1.3803 mL 3.4508 mL
15 mM 0.0920 mL 0.4601 mL 0.9202 mL 2.3005 mL
20 mM 0.0690 mL 0.3451 mL 0.6902 mL 1.7254 mL
25 mM 0.0552 mL 0.2761 mL 0.5521 mL 1.3803 mL
30 mM 0.0460 mL 0.2301 mL 0.4601 mL 1.1503 mL
40 mM 0.0345 mL 0.1725 mL 0.3451 mL 0.8627 mL
50 mM 0.0276 mL 0.1380 mL 0.2761 mL 0.6902 mL
60 mM 0.0230 mL 0.1150 mL 0.2301 mL 0.5751 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cyclic-di-GMP diammonium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclic-di-GMP diammonium609343-82-0c-di-GMP diammoniumcyclic diguanylate diammonium5GP-5GP diammoniumSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

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