Cyclic-di-GMP diammonium (Synonyms: c-di-GMP diammonium; cyclic diguanylate diammonium; 5GP-5GP diammonium)

Name: Cyclic-di-GMP diammonium
Brand: MedChemExpress
SKU: HY-107780B
Rating: 5.0 (9 reviews)

Cat. No.: HY-107780B Purity: 99.07%: Data Sheet
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Cyclic-di-GMP diammonium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP diammonium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP diammonium can be used in cancer research.

For research use only. We do not sell to patients.

CAS No. : 609343-82-0

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Cyclic-di-GMP diammonium:

9 Publications Citing Use of MCE Cyclic-di-GMP diammonium

Bio/Physico-chemical Assay

Histological Imaging/Staining

Gel Electrophoresis

In Vivo Efficacy Study

: Cyclic-di-GMP diammonium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]; Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) increased the protein expression of GSDMD-N, cleaved caspase-3, and phosphorylated-IRF3 (p-IRF3) in C57BL/6 N mice (Pan02-luc cells).

: Cyclic-di-GMP diammonium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]; Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) indicated the good biocompatibility in C57BL/6 N mice (Pan02-luc cells) by H&E staining of the major organs.

: Cyclic-di-GMP diammonium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]; Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) showed antitumour effect in C57BL/6 N mice (Pan02-luc cells).

: Cyclic-di-GMP diammonium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]; agarose gel electrophoresis analysis of DNA degradation assays. Nuclease activity assays were performed by incubating 0.5 μM VcCap5 with 20 ng/μl DNA in the presence of 0.5 μM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

: Cyclic-di-GMP diammonium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]; second messenger-dependent activation of VcCap5 DNA cleavage. Nuclease activity assays were performed by incubating 1 µM VcCap5 with 10 ng/µl DNA in the presence of 1 µM or 5 µM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

: Cyclic-di-GMP diammonium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]; dose-response analysis of VcCap5 activity with increasing concentrations of cyclic dinucleotides. The nuclease assay mixture contained 1 µM VcCap5, 10 ng/µl DNA, and cyclic dinucleotide (Cyclic-di-GMP disodium; c-di-GMP) over a concentration range of 0.1 to 15 µM.

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Description

IC₅₀ & Target

STING^[1]^[2]^[3]^[4].

In Vitro

Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells^[1].
Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells^[2].
Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	H508 cells
Concentration:	0.5-50 µM
Incubation Time:	5 days
Result:	Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay^[2]

Cell Line:	Jurkat cells
Concentration:	50 µM
Incubation Time:	24 h
Result:	Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:	Jurkat cells
Concentration:	50 µM
Incubation Time:	24 h
Result:	Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.

In Vivo

Cyclic-di-GMP diammonium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (B6) mice (8- to 10-week-old)^[3].
Dosage:	100 µg/per
Administration:	Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result:	Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC). Enhanced the anti-tumor activity of TriVax.

Molecular Weight

724.47

Formula

C₂₀H₃₀N₁₂O₁₄P₂

CAS No.

609343-82-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP([O-])(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)=O)[C@H]3COP7([O-])=O.[NH4+].[NH4+]

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (69.02 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3803 mL	6.9016 mL	13.8032 mL
5 mM	0.2761 mL	1.3803 mL	2.7606 mL
10 mM	0.1380 mL	0.6902 mL	1.3803 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (138.03 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.07%

Select Batch:

Data Sheet (284 KB) SDS (252 KB)

COA (255 KB) RP-HPLC (262 KB)

Handling Instructions (2659 KB)

References

[1]. Karaolis DK, et al. 3',5'-Cyclic diguanylic acid (c-di-GMP) inhibits basal and growth factor-stimulated human colon cancer cell proliferation. Biochem Biophys Res Commun. 2005 Apr 1;329(1):40-5. [Content Brief]

[2]. Steinberger O, et al. Elevated expression of the CD4 receptor and cell cycle arrest are induced in Jurkat cells by treatment with the novel cyclic dinucleotide 3',5'-cyclic diguanylic acid. FEBS Lett. 1999 Feb 5;444(1):125-9. [Content Brief]

[3]. Wang Z, et al. STING activator c-di-GMP enhances the anti-tumor effects of peptide vaccines in melanoma-bearing mice. Cancer Immunol Immunother. 2015 Aug;64(8):1057-66. [Content Brief]

[4]. Jenal U, et al. Cyclic di-GMP: second messenger extraordinaire. Nat Rev Microbiol. 2017 May;15(5):271-284. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.3803 mL	6.9016 mL	13.8032 mL	34.5080 mL
	5 mM	0.2761 mL	1.3803 mL	2.7606 mL	6.9016 mL
	10 mM	0.1380 mL	0.6902 mL	1.3803 mL	3.4508 mL
	15 mM	0.0920 mL	0.4601 mL	0.9202 mL	2.3005 mL
	20 mM	0.0690 mL	0.3451 mL	0.6902 mL	1.7254 mL
	25 mM	0.0552 mL	0.2761 mL	0.5521 mL	1.3803 mL
	30 mM	0.0460 mL	0.2301 mL	0.4601 mL	1.1503 mL
	40 mM	0.0345 mL	0.1725 mL	0.3451 mL	0.8627 mL
	50 mM	0.0276 mL	0.1380 mL	0.2761 mL	0.6902 mL
	60 mM	0.0230 mL	0.1150 mL	0.2301 mL	0.5751 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cyclic-di-GMP diammonium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cyclic-di-GMP diammonium (Synonyms: c-di-GMP diammonium; cyclic diguanylate diammonium; 5GP-5GP diammonium)

Customer Review

Other Forms of Cyclic-di-GMP diammonium:

Cyclic-di-GMP diammonium Related Antibodies

9 Publications Citing Use of MCE Cyclic-di-GMP diammonium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Cyclic-di-GMP diammonium Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cyclic-di-GMP diammonium Related Classifications

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Customer Review

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