Cat. No.: HY-107780A

Cyclic-di-GMP sodium

(Synonyms: c-di-GMP sodium; cyclic diguanylate sodium; 5GP-5GP sodium)

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Cyclic-di-GMP sodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP sodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP sodium can be used in cancer research.

For research use only. We do not sell to patients.

Formula: C₂₀H₂₄N₁₀NaO₁₄P₂

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (281 KB)

Cyclic-di-GMP sodium Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (9)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

STING^[1]^[2]^[3]^[4].

All Endogenous Metabolite Isoforms :

In Vitro

Cyclic-di-GMP sodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells^[1].
Cyclic-di-GMP sodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells^[2].
Cyclic-di-GMP sodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

H508 cells
Concentration:

0.5-50 µM
Incubation Time:

5 days
Result:

Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay^[2]

Cell Line:

Jurkat cells
Concentration:

50 µM
Incubation Time:

24 h
Result:

Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:

Jurkat cells
Concentration:

50 µM
Incubation Time:

24 h
Result:

Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.

In Vivo

Cyclic-di-GMP sodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (B6) mice (8- to 10-week-old)^[3].
Dosage:

100 µg/per
Administration:

Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result:

Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax.

Chemical Information

Formula C₂₀H₂₄N₁₀NaO₁₄P₂
SMILES

O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP(O)(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)=O)[C@H]3COP7(O)=O.[Na].[x]
Synonyms

c-di-GMP sodium; cyclic diguanylate sodium; 5GP-5GP sodium
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Microorganisms
- Endogenous metabolite
bacterial species
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (9)

All WBBio/Physico-chemical AssayHistological Imaging/StainingGel ElectrophoresisIn Vivo Efficacy Study

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Journal Impact Factor

Cell

Divergent tumor immunity determined by bacteria-cancer cell engagement. [Abstract]

2026 Mar 19;189(6):1748-1767.e26. PMID: 41643675
Nucleic Acids Res

Cyclic-di-GMP interferes with DNA-MucR-DNA bridging to derepress genes targeted by the xenogeneic silencer MucR. [Abstract]

2025 Oct 28;53(20):gkaf1069. PMID: 41148150
Gut Microbes

Gut microbiota modulate radiotherapy-associated antitumor immune responses against hepatocellular carcinoma Via STING signaling. [Abstract]

2022 Jan-Dec;14(1):2119055. PMID: 36093568
NPJ Biofilms Microbiomes

Bacterial c-di-GMP triggers metamorphosis of mussel larvae through a STING receptor. [Abstract]

2024 Jun 20;10(1):51. PMID: 38902226
Int J Biol Macromol

C-di-GMP@ZIF-8 nanocomposite injectable hydrogel based on modified chitosan and hyaluronic acid for infected wound healing by activating STING signaling. [Abstract]

2024 Sep 14:135660. PMID: 39284469
J Transl Med

Photothermal therapy combined with a STING agonist induces pyroptosis, and gasdermin D could be a new biomarker for guiding the treatment of pancreatic cancer. [Abstract]

2025 Mar 4;23(1):271. PMID: 40038726

Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]

Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) increased the protein expression of GSDMD-N, cleaved caspase-3, and phosphorylated-IRF3 (p-IRF3) in C57BL/6 N mice (Pan02-luc cells).

Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]

Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) indicated the good biocompatibility in C57BL/6 N mice (Pan02-luc cells) by H&E staining of the major organs.

Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]

Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) showed antitumour effect in C57BL/6 N mice (Pan02-luc cells).
Clin Transl Med

Cyclic-di-GMP induces inflammation and acute lung injury through direct binding to MD2. [Abstract]

2024 Aug;14(8):e1744. PMID: 39166890
Mbio

Global Transcriptional Repression of Diguanylate Cyclases by MucR1 Is Essential for Sinorhizobium-Soybean Symbiosis. [Abstract]

2021 Oct 26;12(5):e0119221. PMID: 34700374
J Biol Chem

Dynamic control of bacterial antiphage defense through the CdnG-Cap5 cyclic oligonucleotide-based antiphage pathway in Vibrio cholerae. [Abstract]

2025 Dec 6;302(1):111021. PMID: 41360261

Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]

agarose gel electrophoresis analysis of DNA degradation assays. Nuclease activity assays were performed by incubating 0.5 μM VcCap5 with 20 ng/μl DNA in the presence of 0.5 μM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]

second messenger-dependent activation of VcCap5 DNA cleavage. Nuclease activity assays were performed by incubating 1 µM VcCap5 with 10 ng/µl DNA in the presence of 1 µM or 5 µM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]

dose-response analysis of VcCap5 activity with increasing concentrations of cyclic dinucleotides. The nuclease assay mixture contained 1 µM VcCap5, 10 ng/µl DNA, and cyclic dinucleotide (Cyclic-di-GMP disodium; c-di-GMP) over a concentration range of 0.1 to 15 µM.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Karaolis DK, et al. 3',5'-Cyclic diguanylic acid (c-di-GMP) inhibits basal and growth factor-stimulated human colon cancer cell proliferation. Biochem Biophys Res Commun. 2005 Apr 1;329(1):40-5. [Content Brief]

[2]. Steinberger O, et al. Elevated expression of the CD4 receptor and cell cycle arrest are induced in Jurkat cells by treatment with the novel cyclic dinucleotide 3',5'-cyclic diguanylic acid. FEBS Lett. 1999 Feb 5;444(1):125-9. [Content Brief]

[3]. Wang Z, et al. STING activator c-di-GMP enhances the anti-tumor effects of peptide vaccines in melanoma-bearing mice. Cancer Immunol Immunother. 2015 Aug;64(8):1057-66. [Content Brief]

[4]. Jenal U, et al. Cyclic di-GMP: second messenger extraordinaire. Nat Rev Microbiol. 2017 May;15(5):271-284. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Cyclic-di-GMP sodium

9 Cited Publications

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Cyclic-di-GMP sodium

Publications Citing Use of MedChemExpress (MCE) Cyclic-di-GMP sodium

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (9)

Purity & Documentation

References

Calculators

Help & FAQs

Cyclic-di-GMP sodium Related Classifications

HY-107780A Related Classifications