2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP sodium

Cyclic-di-GMP sodium  (Synonyms: c-di-GMP sodium; cyclic diguanylate sodium; 5GP-5GP sodium)

Cat. No.: HY-107780A
Handling Instructions Technical Support

Cyclic-di-GMP sodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP sodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP sodium can be used in cancer research.

For research use only. We do not sell to patients.

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Cyclic-di-GMP sodium:

Other Forms of Cyclic-di-GMP sodium:

Cyclic-di-GMP sodium Related Antibodies

Top Publications Citing Use of Products

    Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) increased the protein expression of GSDMD-N, cleaved caspase-3, and phosphorylated-IRF3 (p-IRF3) in C57BL/6 N mice (Pan02-luc cells).

    Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) indicated the good biocompatibility in C57BL/6 N mice (Pan02-luc cells) by H&E staining of the major organs.

    Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) showed antitumour effect in C57BL/6 N mice (Pan02-luc cells).

    Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    agarose gel electrophoresis analysis of DNA degradation assays. Nuclease activity assays were performed by incubating 0.5 μM VcCap5 with 20 ng/μl DNA in the presence of 0.5 μM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

    Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    second messenger-dependent activation of VcCap5 DNA cleavage. Nuclease activity assays were performed by incubating 1 µM VcCap5 with 10 ng/µl DNA in the presence of 1 µM or 5 µM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

    Cyclic-di-GMP sodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    dose-response analysis of VcCap5 activity with increasing concentrations of cyclic dinucleotides. The nuclease assay mixture contained 1 µM VcCap5, 10 ng/µl DNA, and cyclic dinucleotide (Cyclic-di-GMP disodium; c-di-GMP) over a concentration range of 0.1 to 15 µM.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Cyclic-di-GMP sodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP sodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP sodium can be used in cancer research[1][2][3][4].

    IC50 & Target
    In Vitro

    Cyclic-di-GMP sodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
    Cyclic-di-GMP sodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
    Cyclic-di-GMP sodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cyclic-di-GMP sodium Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: H508 cells
    Concentration: 0.5-50 µM
    Incubation Time: 5 days
    Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

    Cell Viability Assay[2]

    Cell Line: Jurkat cells
    Concentration: 50 µM
    Incubation Time: 24 h
    Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

    Cell Cycle Analysis[2]

    Cell Line: Jurkat cells
    Concentration: 50 µM
    Incubation Time: 24 h
    Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
    In Vivo

    Cyclic-di-GMP sodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
    Dosage: 100 µg/per
    Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
    Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
    Enhanced the anti-tumor activity of TriVax.
    Formula

    C20H24N10NaO14P2
    SMILES

    O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP(O)(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)=O)[C@H]3COP7(O)=O.[Na].[x]
    Structure Classification
    Initial Source

    bacterial species
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Cyclic-di-GMP sodium Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cyclic-di-GMPc-di-GMPcyclic diguanylate5GP-5GPSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Cyclic-di-GMP sodium
    Cat. No.:
    HY-107780A
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.