2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP disodium

Cyclic-di-GMP disodium  (Synonyms: c-di-GMP disodium; cyclic diguanylate disodium; 5GP-5GP disodium)

Cat. No.: HY-110382 Purity: 99.23%
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Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research.

For research use only. We do not sell to patients.

Cyclic-di-GMP disodium Chemical Structure

Cyclic-di-GMP disodium Chemical Structure

CAS No. : 2222132-40-1

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1 mg USD 170 In-stock
5 mg USD 450 In-stock
10 mg USD 620 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of Cyclic-di-GMP disodium:

Cyclic-di-GMP disodium Related Antibodies

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  • Purity & Documentation

  • References

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Description

Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research[1][2][3][4].

IC50 & Target
In Vitro

Cyclic-di-GMP disodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
? Cyclic-di-GMP disodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
? Cyclic-di-GMP disodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclic-di-GMP disodium Related Antibodies

Cell Proliferation Assay[1]

Cell Line: H508 cells
Concentration: 0.5-50 µM
Incubation Time: 5 days
Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
In Vivo

Cyclic-di-GMP disodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
Dosage: 100 µg/per
Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax.
Molecular Weight

734.37

Formula

C20H22N10Na2O14P2
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)(O[Na])=O)[C@H]3COP7(O[Na])=O
Structure Classification
Initial Source
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light, stored under nitrogen
Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (217.87 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3617 mL 6.8086 mL 13.6171 mL
5 mM 0.2723 mL 1.3617 mL 2.7234 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (68.09 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.3617 mL 6.8086 mL 13.6171 mL 34.0428 mL
5 mM 0.2723 mL 1.3617 mL 2.7234 mL 6.8086 mL
10 mM 0.1362 mL 0.6809 mL 1.3617 mL 3.4043 mL
15 mM 0.0908 mL 0.4539 mL 0.9078 mL 2.2695 mL
20 mM 0.0681 mL 0.3404 mL 0.6809 mL 1.7021 mL
25 mM 0.0545 mL 0.2723 mL 0.5447 mL 1.3617 mL
30 mM 0.0454 mL 0.2270 mL 0.4539 mL 1.1348 mL
40 mM 0.0340 mL 0.1702 mL 0.3404 mL 0.8511 mL
50 mM 0.0272 mL 0.1362 mL 0.2723 mL 0.6809 mL
60 mM 0.0227 mL 0.1135 mL 0.2270 mL 0.5674 mL
80 mM 0.0170 mL 0.0851 mL 0.1702 mL 0.4255 mL
100 mM 0.0136 mL 0.0681 mL 0.1362 mL 0.3404 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cyclic-di-GMP disodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclic-di-GMP disodium2222132-40-1c-di-GMP disodiumcyclic diguanylate disodium5GP-5GP disodiumSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

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