Cyclic-di-GMP disodium (Synonyms: c-di-GMP disodium; cyclic diguanylate disodium; 5GP-5GP disodium)

Name: Cyclic-di-GMP disodium
Brand: MedChemExpress
SKU: HY-110382
Rating: 5.0 (9 reviews)

Cat. No.: HY-110382 Purity: 98.78%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research.

For research use only. We do not sell to patients.

CAS No. : 2222132-40-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Cyclic-di-GMP disodium:

Cyclic-di-GMP diammonium In-stock
Cyclic-di-GMP-¹³C₂₀,¹⁵N₁₀ disodium Get quote
Cyclic-di-GMP Get quote
Cyclic-di-GMP sodium Get quote

9 Publications Citing Use of MCE Cyclic-di-GMP disodium

Bio/Physico-chemical Assay

Histological Imaging/Staining

Gel Electrophoresis

In Vivo Efficacy Study

: Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]; Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) increased the protein expression of GSDMD-N, cleaved caspase-3, and phosphorylated-IRF3 (p-IRF3) in C57BL/6 N mice (Pan02-luc cells).

: Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]; Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) indicated the good biocompatibility in C57BL/6 N mice (Pan02-luc cells) by H&E staining of the major organs.

: Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271. [Abstract]; Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) showed antitumour effect in C57BL/6 N mice (Pan02-luc cells).

: Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]; agarose gel electrophoresis analysis of DNA degradation assays. Nuclease activity assays were performed by incubating 0.5 μM VcCap5 with 20 ng/μl DNA in the presence of 0.5 μM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

: Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]; second messenger-dependent activation of VcCap5 DNA cleavage. Nuclease activity assays were performed by incubating 1 µM VcCap5 with 10 ng/µl DNA in the presence of 1 µM or 5 µM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

: Cyclic-di-GMP disodium purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021. [Abstract]; dose-response analysis of VcCap5 activity with increasing concentrations of cyclic dinucleotides. The nuclease assay mixture contained 1 µM VcCap5, 10 ng/µl DNA, and cyclic dinucleotide (Cyclic-di-GMP disodium; c-di-GMP) over a concentration range of 0.1 to 15 µM.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

STING^[1]^[2]^[3]^[4].

In Vitro

Cyclic-di-GMP disodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells^[1].
Cyclic-di-GMP disodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells^[2].
Cyclic-di-GMP disodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	H508 cells
Concentration:	0.5-50 µM
Incubation Time:	5 days
Result:	Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay^[2]

Cell Line:	Jurkat cells
Concentration:	50 µM
Incubation Time:	24 h
Result:	Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:	Jurkat cells
Concentration:	50 µM
Incubation Time:	24 h
Result:	Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.

In Vivo

Cyclic-di-GMP disodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (B6) mice (8- to 10-week-old)^[3].
Dosage:	100 µg/per
Administration:	Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result:	Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC). Enhanced the anti-tumor activity of TriVax.

Molecular Weight

734.37

Formula

C₂₀H₂₂N₁₀Na₂O₁₄P₂

CAS No.

2222132-40-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)(O[Na])=O)[C@H]3COP7(O[Na])=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Shipping with dry ice.

Storage

-80°C, protect from light, stored under nitrogen

Solvent & Solubility

In Vitro:

H₂O : 160 mg/mL (217.87 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3617 mL	6.8086 mL	13.6171 mL
5 mM	0.2723 mL	1.3617 mL	2.7234 mL
10 mM	0.1362 mL	0.6809 mL	1.3617 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (68.09 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.78%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (254 KB) HNMR (378 KB) RP-HPLC (308 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Karaolis DK, et al. 3',5'-Cyclic diguanylic acid (c-di-GMP) inhibits basal and growth factor-stimulated human colon cancer cell proliferation. Biochem Biophys Res Commun. 2005 Apr 1;329(1):40-5. [Content Brief]

[2]. Steinberger O, et al. Elevated expression of the CD4 receptor and cell cycle arrest are induced in Jurkat cells by treatment with the novel cyclic dinucleotide 3',5'-cyclic diguanylic acid. FEBS Lett. 1999 Feb 5;444(1):125-9. [Content Brief]

[3]. Wang Z, et al. STING activator c-di-GMP enhances the anti-tumor effects of peptide vaccines in melanoma-bearing mice. Cancer Immunol Immunother. 2015 Aug;64(8):1057-66. [Content Brief]

[4]. Jenal U, et al. Cyclic di-GMP: second messenger extraordinaire. Nat Rev Microbiol. 2017 May;15(5):271-284. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.3617 mL	6.8086 mL	13.6171 mL	34.0428 mL
	5 mM	0.2723 mL	1.3617 mL	2.7234 mL	6.8086 mL
	10 mM	0.1362 mL	0.6809 mL	1.3617 mL	3.4043 mL
	15 mM	0.0908 mL	0.4539 mL	0.9078 mL	2.2695 mL
	20 mM	0.0681 mL	0.3404 mL	0.6809 mL	1.7021 mL
	25 mM	0.0545 mL	0.2723 mL	0.5447 mL	1.3617 mL
	30 mM	0.0454 mL	0.2270 mL	0.4539 mL	1.1348 mL
	40 mM	0.0340 mL	0.1702 mL	0.3404 mL	0.8511 mL
	50 mM	0.0272 mL	0.1362 mL	0.2723 mL	0.6809 mL
	60 mM	0.0227 mL	0.1135 mL	0.2270 mL	0.5674 mL
	80 mM	0.0170 mL	0.0851 mL	0.1702 mL	0.4255 mL
	100 mM	0.0136 mL	0.0681 mL	0.1362 mL	0.3404 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cyclic-di-GMP disodium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Cyclic-di-GMP disodium (Synonyms: c-di-GMP disodium; cyclic diguanylate disodium; 5GP-5GP disodium)

Customer Review

Other Forms of Cyclic-di-GMP disodium:

Cyclic-di-GMP disodium Related Antibodies

9 Publications Citing Use of MCE Cyclic-di-GMP disodium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Cyclic-di-GMP disodium Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cyclic-di-GMP disodium Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report