2. Anti-infection Metabolic Enzyme/Protease
  3. Bacterial Fungal Aldehyde Dehydrogenase (ALDH)
  4. Dyclonine hydrochloride

Dyclonine (hydrochloride)  (Synonyms: Dyclocaine hydrochloride; ダイクロニン塩酸塩)

製品番号: HY-B0364A 純度: 99.30%
COA 取扱説明書 Technical Support

Dyclonine (Dyclocaine) hydrochloride is an orally effective ALDH covalent inhibitor (crosses blood-brain barrier), with an IC50 of 35 μM for ALDH2 and 76 μM for ALDH3A1. Dyclonine hydrochloride has sensitizing activities for targeted cancer cells and antibacterial. Dyclonine hydrochloride is also a local agent that can suppress or relieve pain. that blocks the transmission of various nerve impulses or stimuli and inhibits the sensation of touch and pain.

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Dyclonine hydrochloride

Dyclonine (hydrochloride) 構造式

CAS 番号 : 536-43-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
500 mg $55 在庫あり
5 g $66 在庫あり
10 g $92 在庫あり
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

Other Forms of Dyclonine hydrochloride:

Dyclonine (hydrochloride) 関連抗体

Top Publications Citing Use of Products

Aldehyde Dehydrogenase (ALDH) アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
ALDH1 ALDH2 ALDH3

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Dyclonine (Dyclocaine) hydrochloride is an orally effective ALDH covalent inhibitor (crosses blood-brain barrier), with an IC50 of 35 μM for ALDH2 and 76 μM for ALDH3A1. Dyclonine hydrochloride has sensitizing activities for targeted cancer cells and antibacterial. Dyclonine hydrochloride is also a local agent that can suppress or relieve pain. that blocks the transmission of various nerve impulses or stimuli and inhibits the sensation of touch and pain[1][2][3].

IC50 & Target[3]

ALDH2

35 μM (IC50)

ALDH3

76 μM (IC50)

体外実験

Dyclonine hydrochloride (50 μM; 24 h) sensitizes cancer cells to deficiency of cysteine and GSH[1].
Dyclonine hydrochloride (0-2048 μg/mL; 24 or 48 h) shows significant bactericidal and fungicidal activity[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dyclonine (hydrochloride) 関連抗体

Cell Viability Assay[1]

Cell Line: HSC-4 cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Inhibited ALDH activity and induced 4-HNE accumulation in GSH-depleted cancer cells.

Cell Viability Assay[2]

Cell Line: S. aureus, B. subtilis, E. coli, T. mentagrophyte
Concentration: 0-2048 µg/mL
Incubation Time: 24 h (for bacterial), 48 h (for fungi)
Result: Showed good antibacterial and antifungal activity, with minimum microbicidal concentration of 0.006%, 0.025%, 0.012% and 0.025% for S. aureus, B. subtilis, E. coli, T. mentagrophyte, separately.
体内実験

Dyclonine hydrochloride (5 mg/kg; i.p.; single daily for 24 days) sensitizes the involucrin+ differentiated tumor cells to sulfasalazine treatment in vivo[1].
Dyclonine hydrochloride (5 mg/kg; i.p.; single daily for 21 days) suppress the growth of tumors formed by ALDH3A1-expressing gastric cancer stemlike cells when combines with sulfasalazine[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude mice (HSC-2 cells implanted model)[1].
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; single daily for 24 days (combine with sulfasalazine).
Result: Attenuated the formation of tumors by HSC-2 cells implanted in nude mice when combined with sulfasalazine.
Animal Model: C57BL6 mice (K19-Wnt1/C2mE-KP cells implanted model)[1].
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; single daily for 21 days (combine with sulfasalazine).
Result: Enhanced the antitumor effect of sulfasalazine on HNSCC tumors consisting of CD44vhigh stemlike tumor cells and involucrin+ differentiated tumor cells.
臨床実験
分子量

325.87

分子式

C18H28ClNO2
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(OCCCC)C=C1)CCN2CCCCC2.Cl
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

H2O : 50 mg/mL (153.44 mM; Need ultrasonic)

DMSO : ≥ 25 mg/mL (76.72 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0687 mL 15.3435 mL 30.6871 mL
5 mM 0.6137 mL 3.0687 mL 6.1374 mL
10 mM 0.3069 mL 1.5344 mL 3.0687 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.30%

COA (243 KB) LCMS (104 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.0687 mL 15.3435 mL 30.6871 mL 76.7177 mL
5 mM 0.6137 mL 3.0687 mL 6.1374 mL 15.3435 mL
10 mM 0.3069 mL 1.5344 mL 3.0687 mL 7.6718 mL
15 mM 0.2046 mL 1.0229 mL 2.0458 mL 5.1145 mL
20 mM 0.1534 mL 0.7672 mL 1.5344 mL 3.8359 mL
25 mM 0.1227 mL 0.6137 mL 1.2275 mL 3.0687 mL
30 mM 0.1023 mL 0.5115 mL 1.0229 mL 2.5573 mL
40 mM 0.0767 mL 0.3836 mL 0.7672 mL 1.9179 mL
50 mM 0.0614 mL 0.3069 mL 0.6137 mL 1.5344 mL
60 mM 0.0511 mL 0.2557 mL 0.5115 mL 1.2786 mL
H2O 80 mM 0.0384 mL 0.1918 mL 0.3836 mL 0.9590 mL
100 mM 0.0307 mL 0.1534 mL 0.3069 mL 0.7672 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dyclonine hydrochloride Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dyclonine536-43-6DyclocaineBacterialFungalAldehyde Dehydrogenase (ALDH)HSC-4 cellsS. aureusB. subtilisE. coliT. mentagrophyteanestheticcancerInhibitorinhibitorinhibit

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