GSK-3174998 

GSK-3174998 is a humanized IgG1 OX40/TNFRSF4 agonistic monoclonal antibody. GSK-3174998 has the potential for the study of advanced solid tumors. The isotype control for GSK-3174998 can refer to Human IgG1 kappa, Isotype Control (HY-P99001)^[1].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

TNFRSF4/OX40/CD134

7293  [NCBI]

P43489

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

147.197 kDa

2089398-68-3

Liquid

Colorless to light yellow

[GSK-3174998]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Immobilized OX40/TNFRSF4 Protein, Human (HEK293, His, HY-P7394) can bind GSK-3174998. The EC₅₀ for this effect is 5.769 ng/mL.
• 
  Flow cytometric analysis of 1X10⁶ Juakat cells with GSK-3174998 (HY-P991052, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Postel-Vinay S, et al. First-in-human phase I study of the OX40 agonist GSK3174998 with or without pembrolizumab in patients with selected advanced solid tumors (ENGAGE-1). J Immunother Cancer. 2023 Mar;11(3):e005301.  [Content Brief]