Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)  

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

MCE Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) is a mixture of multiple phosphatase inhibitors that effectively inhibit serine/threonine phosphatases and alkaline phosphatases, and is used to preserve the phosphorylation status of proteins. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.

-20℃, 2 years

Thaw at room temperature, add the inhibitor mixture to the lysate at 1:100 (v/v), and mix thoroughly.