Izalontamab  (Synonyms: SI-B001; BL-B01D1 Antibody)

Izalontamab (SI-B001) is a bispecific anti-EGFR/HER3 monoclonal antibody with high selectivity for EGFR/HER3 heterodimer. Izalontamab can be used for the research of cancer^[1].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

EGFR & ERBB3

Izalontamab (5-46 mg/kg; i.v., every 7 days once, 4 doses in total) shows different anti-tumor efficacy in different types of tumors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1956  [NCBI] & 2065  [NCBI]

P00533 & P21860

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

197.18 kDa

2559704-24-2

Liquid

Colorless to light yellow

[Izalontamab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  IgG1-kappa-[scFv-heavy-lambda]2

• 
  Immobilized Human HER3/ERBB3 Protein (ECD, His Tag) can bind Izalontamab. The EC₅₀ for this effect is 1.98 ng/mL.
• 
  Immobilized Human EGFR Protein (ECD, His Tag) can bind Izalontamab. The EC₅₀ for this effect is 2.53 ng/mL.
• 
  Flow Cytometry analysis of Hela cells labelling EGFR (red) with Izalontamab (HY-P99676). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
• 
  Flow Cytometry analysis of MCF-7 cells labelling ERBB3 (red) with Izalontamab (HY-P99676). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Xue J, et al. Prediction of Human Pharmacokinetics and Clinical Effective Dose of SI-B001, an EGFR/HER3 Bi-specific Monoclonal Antibody. J Pharm Sci. 2020 Oct;109(10):3172-3180.  [Content Brief]