2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. L-Leucyl-L-Leucine methyl ester hydrobromide

L-Leucyl-L-Leucine methyl ester (hydrobromide)  (Synonyms: LLOMe (hydrobromide); Leu-Leu methyl ester (hydrobromide); H-Leu-Leu-OMe (hydrobromide))

製品番号: HY-129905A 純度: 99.69%
COA 取扱説明書 Technical Support

L-Leucyl-L-Leucine methyl ester (LLOMe) hydrobromide, a dipeptide condensation product of L-leucine methyl ester generated by human monocytes or polymorphonuclear leukocytes, selectively eliminates lymphocytes with cytotoxic potential. L-Leucyl-L-Leucine methyl ester hydrobromide also can induce endolysosomal pathway stress.

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CAS 番号 : 16689-14-8

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
5 mg $50 在庫あり
10 mg $80 在庫あり
25 mg $150 在庫あり
50 mg $225 在庫あり
100 mg $330 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 18 publication(s) in Google Scholar

Other Forms of L-Leucyl-L-Leucine methyl ester hydrobromide:

L-Leucyl-L-Leucine methyl ester (hydrobromide) 関連抗体

Top Publications Citing Use of Products

    L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: EMBO J. 2025 May;44(9):2596-2619.  [Abstract]

    S-palmitoylation of HA-ATG2A detected by ABE assay in HEK293T cells treated with 1 mM LLOMe for indicated times.

    L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    U2OS cells were transfected with HaloTag-LRRK2 for 36 h. Cells were then treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h before fixation. Cells were then stained for RILPL1 and LAMP2.

    L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    Quantification of the RILPL1 lysosomes per cell. Cells were treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h.

    L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    HEK293FT cells were transfected with 3xflag-LRRK2 and mCherry-RILPL1 for 24 h. Cells were treated or not with LLOME (1 mM) for 2 h, and lysates were subjected to immunoprecipitation with anti-RFP antibodies. RFP, LRRK2, and pT73-RAB10 were blotted.

    L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    HEK293T cells stably expressing GFP-LRRK2 and TMEM192-3xHA were seeded and treated with DMSO, LLOME (1 mM), or LLOME + MLi2 24 h later. Lysosomes were purified with anti-HA beads following the LYSO-IP technique. Lysosomes were then lysed, and their content was analyzed via immunoblotting.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

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    • 純度とドキュメンテーション

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    • カスタマーレビュー

    製品説明

    L-Leucyl-L-Leucine methyl ester (LLOMe) hydrobromide, a dipeptide condensation product of L-leucine methyl ester generated by human monocytes or polymorphonuclear leukocytes, selectively eliminates lymphocytes with cytotoxic potential. L-Leucyl-L-Leucine methyl ester hydrobromide also can induce endolysosomal pathway stress[1][2][3].

    体外実験

    L-Leucyl-L-Leucine methyl ester (1 mM; 0.5-2 h) enhances LRRK2-mediated Rab10 and Rab12 phosphorylation in MEFs and A549 cells[3].
    L-Leucyl-L-Leucine methyl ester (10-250 μM; 15 min) is converted to a CCI3COOH-insoluble product by CD4- lymphocytes[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Leucyl-L-Leucine methyl ester (hydrobromide) 関連抗体
    分子量

    339.27

    分子式

    C13H27BrN2O3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(C)C[C@@H](C(OC)=O)NC([C@H](CC(C)C)N)=O.[H]Br
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 125 mg/mL (368.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (294.75 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9475 mL 14.7375 mL 29.4750 mL
    5 mM 0.5895 mL 2.9475 mL 5.8950 mL
    10 mM 0.2948 mL 1.4738 mL 2.9475 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (294.75 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.69%

    COA (247 KB) HNMR (258 KB) RP-HPLC (223 KB) LCMS (88 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.9475 mL 14.7375 mL 29.4751 mL 73.6876 mL
    5 mM 0.5895 mL 2.9475 mL 5.8950 mL 14.7375 mL
    10 mM 0.2948 mL 1.4738 mL 2.9475 mL 7.3688 mL
    15 mM 0.1965 mL 0.9825 mL 1.9650 mL 4.9125 mL
    20 mM 0.1474 mL 0.7369 mL 1.4738 mL 3.6844 mL
    25 mM 0.1179 mL 0.5895 mL 1.1790 mL 2.9475 mL
    30 mM 0.0983 mL 0.4913 mL 0.9825 mL 2.4563 mL
    40 mM 0.0737 mL 0.3684 mL 0.7369 mL 1.8422 mL
    50 mM 0.0590 mL 0.2948 mL 0.5895 mL 1.4738 mL
    60 mM 0.0491 mL 0.2456 mL 0.4913 mL 1.2281 mL
    80 mM 0.0368 mL 0.1842 mL 0.3684 mL 0.9211 mL
    100 mM 0.0295 mL 0.1474 mL 0.2948 mL 0.7369 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Leucyl-L-Leucine methyl ester hydrobromide Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Leucyl-L-Leucine methyl ester16689-14-8LLOMeLeu-Leu methyl esterH-Leu-Leu-OMeEndogenous MetabolitemonocytespolymorphonuclearleukocyteslymphocytescytotoxicendolysosomalpathwaystressInhibitorinhibitorinhibit

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    製品名:
    L-Leucyl-L-Leucine methyl ester hydrobromide
    製品番号:
    HY-129905A
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