L-Leucyl-L-Leucine methyl ester hydrochloride
Based on 22 publication(s) in Google Scholar
L-Leucyl-L-Leucine methyl ester (LLOMe) hydrochloride, a dipeptide condensation product of L-leucine methyl ester generated by human monocytes or polymorphonuclear leukocytes, selectively eliminates lymphocytes with cytotoxic potential. L-Leucyl-L-Leucine methyl ester hydrochloride also can induce endolysosomal pathway stress.
For research use only. We do not sell to patients.
- Purity : 99.09%
- CAS No.: 6491-83-4
- Formula: C13H27ClN2O3
- Molecular Weight:294.82
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Leucyl-L-Leucine methyl ester hydrochloride
More- Cell Res. 2026 Jan;36(1):51-71. [Abstract]
- Autophagy. 2026 Jul 10:1-21.
- Autophagy. 2024 Dec;20(12):2697-2718. [Abstract]
- Adv Sci (Weinh). 2026 May;13(25):e21681. [Abstract]
- Biomaterials. 2026 Jun 27:335:124405. [Abstract]
- Biomaterials. 2025 Jun:317:123091. [Abstract]
- Environ Chem Ecotoxicol. 2025 Nov 19;8:267-280.
- J Neuroinflammation. 2025 Oct 29;22(1):252. [Abstract]
- EMBO J. 2025 May;44(9):2596-2619. [Abstract]
- World J Gastroenterol. 2026 Feb 28;32(8):113299. [Abstract]
- J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]
- PLoS Pathog. 2025 Sep 16;21(9):e1013513. [Abstract]
- PLoS Pathog. 2024 Feb 14;20(2):e1011981. [Abstract]
- Toxicol Appl Pharmacol. 2026 Jan:506:117632. [Abstract]
- Int Immunol. 2021 Aug 23;33(9):491-504. [Abstract]
- FEBS Lett. 2026 Apr 24. [Abstract]
- Traffic. 2022 May;23(5):238-269. [Abstract]
- bioRxiv. 2026 Apr 21.
- SSRN. 2025 Aug 13.
- Preprints. 2025 Jul 8.
- bioRxiv. 2024 July 07.
- bioRxiv. 2024 Mar 13:2024.03.12.584718. [Abstract]
Customer Validation & Images
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All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
L-Leucyl-L-Leucine methyl ester (1 mM; 0.5-2 h) enhances LRRK2-mediated Rab10 and Rab12 phosphorylation in MEFs and A549 cells[3].
L-Leucyl-L-Leucine methyl ester (10-250 μM; 15 min) is converted to a CCI3COOH-insoluble product by CD4- lymphocytes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6491-83-4
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Appearance Solid
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Molecular Weight 294.82
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Formula C13H27ClN2O3
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Color White to off-white
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SMILES
CC(C)C[C@@H](C(OC)=O)NC([C@H](CC(C)C)N)=O.[H]Cl
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Synonyms
LLOMe hydrochloride; Leu-Leu methyl ester hydrochloride; H-Leu-Leu-OMe hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (22)
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Journal Impact Factor
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Most Recent
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Cell Res
2026 Jan;36(1):51-71. PMID: 41478856 -
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Autophagy
2024 Dec;20(12):2697-2718. PMID: 39081059 -
Adv Sci (Weinh)
Loss of VMP1 Impairs Tight Junction Recycling and Aggravates Intestinal Barrier Dysfunction in Inflammatory Bowel Disease. [Abstract]2026 May;13(25):e21681. PMID: 41757559 -
Biomaterials
In-situ cascade assembled peptide-drug conjugate for the treatment of bladder cancer by enhancing membrane-entry and lysosome destabilization. [Abstract]2026 Jun 27:335:124405. PMID: 42372507 -
Biomaterials
Engineered cell membrane vesicles loaded with lysosomophilic drug for acute myeloid leukemia therapy via organ-cell-organelle cascade-targeting. [Abstract]2025 Jun:317:123091. PMID: 39778270 -
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J Neuroinflammation
Immune subversion by Leishmania infantum parasites suppresses NLRP3-driven inflammatory responses in amyloid-β-activated microglia. [Abstract]2025 Oct 29;22(1):252. PMID: 41162989 -
EMBO J
S-palmitoylation modulates ATG2-dependent non-vesicular lipid transport during starvation-induced autophagy. [Abstract]2025 May;44(9):2596-2619. PMID: 40128367
L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: EMBO J. 2025 May;44(9):2596-2619. [Abstract]
S-palmitoylation of HA-ATG2A detected by ABE assay in HEK293T cells treated with 1 mM LLOMe for indicated times.
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World J Gastroenterol
Wogonin derivative V8 enhances bortezomib efficacy in gastric carcinoma by disrupting lysosome-mediated drug resistance. [Abstract]2026 Feb 28;32(8):113299. PMID: 41809875 -
J Cell Biol
JIP4 and RILPL1 utilize opposing motor force to dynamically regulate lysosomal tubulation. [Abstract]2025 Nov 3;224(11):e202404018. PMID: 40990465
L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]
U2OS cells were transfected with HaloTag-LRRK2 for 36 h. Cells were then treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h before fixation. Cells were then stained for RILPL1 and LAMP2.
L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]
Quantification of the RILPL1 lysosomes per cell. Cells were treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h.
L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]
HEK293FT cells were transfected with 3xflag-LRRK2 and mCherry-RILPL1 for 24 h. Cells were treated or not with LLOME (1 mM) for 2 h, and lysates were subjected to immunoprecipitation with anti-RFP antibodies. RFP, LRRK2, and pT73-RAB10 were blotted.
L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]
HEK293T cells stably expressing GFP-LRRK2 and TMEM192-3xHA were seeded and treated with DMSO, LLOME (1 mM), or LLOME + MLi2 24 h later. Lysosomes were purified with anti-HA beads following the LYSO-IP technique. Lysosomes were then lysed, and their content was analyzed via immunoblotting.
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PLoS Pathog
A non-canonical activation of the host's ESCRT machinery is required for the scission of parasitophorous vacuoles and the replication of Leishmania donovani. [Abstract]2025 Sep 16;21(9):e1013513. PMID: 40956840 -
PLoS Pathog
The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization. [Abstract]2024 Feb 14;20(2):e1011981. PMID: 38354122 -
Toxicol Appl Pharmacol
Honokiol ameliorates hepatic fibrosis by inducing lysosomal membrane permeabilization and impairing lipophagy in hepatic stellate cells. [Abstract]2026 Jan:506:117632. PMID: 41207513 -
Int Immunol
Helicobacter urease suppresses cytotoxic CD8+ T-cell responses through activating Myh9-dependent induction of PD-L1. [Abstract]2021 Aug 23;33(9):491-504. PMID: 34297096 -
FEBS Lett
2026 Apr 24. PMID: 42032884 -
Traffic
2022 May;23(5):238-269. PMID: 35343629 -
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bioRxiv
Autophagosome turnover requires Arp2/3 complex-mediated maintenance of lysosomal integrity. [Abstract]2024 Mar 13:2024.03.12.584718. PMID: 38559247
Solvent & Solubility
In Vitro:
DMSO : 250 mg/mL (847.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Thiele DL, et, al. The immunosuppressive activity of L-leucyl-L-leucine methyl ester: selective ablation of cytotoxic lymphocytes and monocytes. J Immunol. 1986 Feb 1;136(3):1038-48. [Content Brief]
[2]. Thiele DL, et, al. Mechanism of L-leucyl-L-leucine methyl ester-mediated killing of cytotoxic lymphocytes: dependence on a lysosomal thiol protease, dipeptidyl peptidase I, that is enriched in these cells. Proc Natl Acad Sci U S A. 1990 Jan;87(1):83-7. [Content Brief]
[3]. Kalogeropulou AF, et, al. Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity. Biochem J. 2020 Nov 27;477(22):4397-4423. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3919 mL | 16.9595 mL | 33.9190 mL | 84.7975 mL |
| 5 mM | 0.6784 mL | 3.3919 mL | 6.7838 mL | 16.9595 mL | |
| 10 mM | 0.3392 mL | 1.6960 mL | 3.3919 mL | 8.4798 mL | |
| 15 mM | 0.2261 mL | 1.1306 mL | 2.2613 mL | 5.6532 mL | |
| 20 mM | 0.1696 mL | 0.8480 mL | 1.6960 mL | 4.2399 mL | |
| 25 mM | 0.1357 mL | 0.6784 mL | 1.3568 mL | 3.3919 mL | |
| 30 mM | 0.1131 mL | 0.5653 mL | 1.1306 mL | 2.8266 mL | |
| 40 mM | 0.0848 mL | 0.4240 mL | 0.8480 mL | 2.1199 mL | |
| 50 mM | 0.0678 mL | 0.3392 mL | 0.6784 mL | 1.6960 mL | |
| 60 mM | 0.0565 mL | 0.2827 mL | 0.5653 mL | 1.4133 mL | |
| 80 mM | 0.0424 mL | 0.2120 mL | 0.4240 mL | 1.0600 mL | |
| 100 mM | 0.0339 mL | 0.1696 mL | 0.3392 mL | 0.8480 mL |
L-Leucyl-L-Leucine methyl ester hydrochloride Related Classifications
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