1. PI3K/Akt/mTOR Autophagy
  2. mTOR Autophagy
  3. Torin 1

Torin 1 is a potent inhibitor of mTOR with an IC50 of 3 nM. Torin 1 inhibits both mTORC1/2 complexes with IC50 values between 2 and 10 nM. Torin 1 is an effective inducer of autophagy.

For research use only. We do not sell to patients.

Torin 1 Chemical Structure

Torin 1 Chemical Structure

CAS No. : 1222998-36-8

Size Price Stock Quantity
5 mg USD 84 In-stock
10 mg USD 126 In-stock
50 mg USD 400 In-stock
100 mg USD 600 In-stock
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500 mg   Get quote  

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Customer Review

Based on 69 publication(s) in Google Scholar

Top Publications Citing Use of Products

62 Publications Citing Use of MCE Torin 1

WB

    Torin 1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing the cell cycle and autophagy-related proteins after 48 h of treatment with 200 μM NSC 697855 (NTZ) and/or 500 nM Torin 1 or 30 mM Chloroquine (CQ).

    Torin 1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 22;9(6):604.  [Abstract]

    AGS cells are pretreated with 25 and 50 nM PS-341 for 1 h, and then incubated with 500 nM Torin 1 for 24 h. After pretreatment with PPI for 24 h in pH 7.4 or pH 6.5 condition, Rapamycin (1 μM) or Torin 1 (500 nM) is added for another 24 h. The protein level of SQSTM1 is measured by western blot analysis.

    Torin 1 purchased from MedChemExpress. Usage Cited in: J Virol. 2018 Jul 31;92(16). pii: e00600-18.  [Abstract]

    HEK293T cells treated with Torin 1 (250 nM, 6 h) are used as the positive control. The cells are harvested at 30 h posttransfection and the lysates are analyzed with immunoblotting using antibodies specific for the indicated proteins.

    Torin 1 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2018 Nov;43(11):2141-2154.  [Abstract]

    Torin 1 (T) stimulation decreases the ratios of phospho-mTOR (Ser 2448)/ mTOR, phospho-S6K1 (Thr 389)/S6K1 and NGF/β-actin at all time points.

    Torin 1 purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2018 Jun 15;367(2):186-195.  [Abstract]

    Torin1 and Rapamycin downregulate phospho-mTOR (Ser 2448), phospho-S6K1 (Thr 389), phospho-4EBP1 (Thr 37/46), Bcl-2 and upregulate Bax, cleaved caspase-3 in vitro cultured RSC96 cells.

    Torin 1 purchased from MedChemExpress. Usage Cited in: Haematologica. 2017 Apr;102(4):755-764.  [Abstract]

    Knocking out of 4EBPs induces resistance to TORKi treatment. Ramos is transduced with CRISPR-CAS9 vectors targeting both 4EPB1 and 4EBP2 and immunoblotted with the indicated antibodies. 48 h after treatment with AZD8055 or Torin1, 4EBP1/2 double knock-out (Ramos-DKO) and control (Ramos-C) Ramos cells are immunoblotted with antibodies against MCL1 and BCL-XL

    Torin 1 purchased from MedChemExpress. Usage Cited in: Mol Cell Biol. 2017 Jun 29;37(14). pii: e00075-17.  [Abstract]

    L-glutamine-responsive TORC1 activation in vitro. An in vitro kinase assay using semi-intact cells is performed. The reaction is performed with or without the indicated amount of Rapamycin or Torin-1. FLAG-tagged Tor1 and Myc-tagged Kog1 are detected as loading controls. The bar graph refers to the mean ratio of phosphorylated/total 4EBP1, normalized to the values for the DMSO-treated and glutamine-unadded samples.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Torin 1 is a potent inhibitor of mTOR with an IC50 of 3 nM. Torin 1 inhibits both mTORC1/2 complexes with IC50 values between 2 and 10 nM. Torin 1 is an effective inducer of autophagy.

    IC50 & Target[1]

    mTOR

    3 nM (IC50)

    mTORC1

    2-10 nM (IC50)

    mTORC2

    2-10 nM (IC50)

    ATM

    0.6 μM (IC50)

    DNA-PK

    1 μM (IC50)

    PI3K-α

    1.8 μM (IC50)

    hVps34

    3 μM (IC50)

    Autophagy

     

    In Vitro

    Torin1 (250 nM) completely inhibits proliferation and causes a G1/S cell cycle arrest, and decreases cell size to a greater degree than 50 nM rapamycin in wild-type MEFs[1]. Torin1 has more than 800-fold selectivity between mTOR and PI3Kis, and is very selective relative to other PIKK family kinases with the exception of DNA-PK[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Torin1 (20 mg/kg, i.p.) is efficacious in a U87MG xenograft model, and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    607.62

    Formula

    C35H28F3N5O2

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1N(C2=C(C=C1)C=NC3=CC=C(C=C32)C4=CC5=CC=CC=C5N=C4)C6=CC=C(C(C(F)(F)F)=C6)N7CCN(CC7)C(CC)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 2 mg/mL (3.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6458 mL 8.2288 mL 16.4577 mL
    5 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months. When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  4% NMP    3% Tween-80    20% PEG400    73% ddH2O

      Solubility: 10 mg/mL (16.46 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 0.25 mg/mL (0.41 mM); Clear solution

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.95%

    References
    Kinase Assay
    [1]

    To produce soluble mTORC1, HEK-293T cell lines are generated that stably express N-terminally FLAG-tagged Raptor using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, HeLa cells are generated that stably express N-terminally FLAG-tagged Protor-1. Both complexes are purified by lysing cells in 50 mM HEPES, pH 7.4, 10 mM sodium pyrophosphate, 10 mM sodium β-glycerophosphate, 100 mM NaCl, 2 mM EDTA, 0.3% CHAPS. Cells are lysed at 4°C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 mol/L NaCl. Purified mTORC1 is eluted with 100 μg/mL 3× FLAG peptide in 50 mM HEPES, pH 7.4, 100 mM NaCl. Eluate can be aliquoted and stored at -80°C. Kinase assays are performed for 20 min at 30°C in a final volume of 20 μL consisting of the kinase buffer (25 mM HEPES, pH 7.4, 50 mM KCl, 10 mM MgCl2, 500 μM ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    For pharmacodynamic experiments, torin 1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and then diluted 1:4 with sterile 50% PEG400 prior to injection. Six-week old male C57BL/6 mice are fasted overnight prior to drug treatment. The mice are treated with vehicle (for 10 hr) or 26 (20 mg/kg for 2, 6 or 10 hr) by IP injection, and then refed 1 h prior to sacrifice (CO2 asphyxiation). Tissues are collected and frozen on dry ice. The frozen tissue is thawed on ice and lysed by sonication in tissue lysis buffer (50 mM HEPES, pH 7.4, 40 mM NaCl, 2 mM EDTA, 1.5 mM sodium orthovanadate, 50 mM sodium fluoride, 10 mM sodium pyrophosphate, 10 mM sodium β-glycerophosphate, 0.1% SDS, 1.0% sodium deoxycholate and 1.0% Triton, supplemented with protease inhibitor cocktail tablets). The concentration of clear lysate is measured using the Bradford assay and samples are subsequently normalized by protein content and analyzed by SDS-PAGE and immunoblotting.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months. When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6458 mL 8.2288 mL 16.4577 mL 41.1441 mL
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    Torin 1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Cat. No.:
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