L-Leucyl-L-Leucine methyl ester hydrobromide (Synonyms: LLOMe hydrobromide; Leu-Leu methyl ester hydrobromide; H-Leu-Leu-OMe hydrobromide)

Name: L-Leucyl-L-Leucine methyl ester hydrobromide
Brand: MedChemExpress
SKU: HY-129905A
Rating: 5.0 (18 reviews)

Cat. No.: HY-129905A Purity: 99.69%: Data Sheet
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L-Leucyl-L-Leucine methyl ester (LLOMe) hydrobromide, a dipeptide condensation product of L-leucine methyl ester generated by human monocytes or polymorphonuclear leukocytes, selectively eliminates lymphocytes with cytotoxic potential. L-Leucyl-L-Leucine methyl ester hydrobromide also can induce endolysosomal pathway stress.

For research use only. We do not sell to patients.

CAS No. : 16689-14-8

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Customer Review

Based on 18 publication(s) in Google Scholar

Other Forms of L-Leucyl-L-Leucine methyl ester hydrobromide:

L-Leucyl-L-Leucine methyl ester hydrochloride In-stock
Leucylleucine methyl ester Get quote

18 Publications Citing Use of MCE L-Leucyl-L-Leucine methyl ester hydrobromide

Cell Imaging/Staining

: L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: EMBO J. 2025 May;44(9):2596-2619. [Abstract]; S-palmitoylation of HA-ATG2A detected by ABE assay in HEK293T cells treated with 1 mM LLOMe for indicated times.

: L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]; U2OS cells were transfected with HaloTag-LRRK2 for 36 h. Cells were then treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h before fixation. Cells were then stained for RILPL1 and LAMP2.

: L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]; Quantification of the RILPL1 lysosomes per cell. Cells were treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h.

: L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]; HEK293FT cells were transfected with 3xflag-LRRK2 and mCherry-RILPL1 for 24 h. Cells were treated or not with LLOME (1 mM) for 2 h, and lysates were subjected to immunoprecipitation with anti-RFP antibodies. RFP, LRRK2, and pT73-RAB10 were blotted.

: L-Leucyl-L-Leucine methyl ester hydrobromide purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018. [Abstract]; HEK293T cells stably expressing GFP-LRRK2 and TMEM192-3xHA were seeded and treated with DMSO, LLOME (1 mM), or LLOME + MLi2 24 h later. Lysosomes were purified with anti-HA beads following the LYSO-IP technique. Lysosomes were then lysed, and their content was analyzed via immunoblotting.

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Description

In Vitro

L-Leucyl-L-Leucine methyl ester (1 mM; 0.5-2 h) enhances LRRK2-mediated Rab10 and Rab12 phosphorylation in MEFs and A549 cells^[3].
L-Leucyl-L-Leucine methyl ester (10-250 μM; 15 min) is converted to a CCI₃COOH-insoluble product by CD4^- lymphocytes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

339.27

Formula

C₁₃H₂₇BrN₂O₃

CAS No.

16689-14-8

Appearance

Solid

Color

White to off-white

SMILES

CC(C)C[C@@H](C(OC)=O)NC([C@H](CC(C)C)N)=O.[H]Br

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (368.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (294.75 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9475 mL	14.7375 mL	29.4750 mL
5 mM	0.5895 mL	2.9475 mL	5.8950 mL
10 mM	0.2948 mL	1.4738 mL	2.9475 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (294.75 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (247 KB) HNMR (258 KB) RP-HPLC (223 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Thiele DL, et, al. The immunosuppressive activity of L-leucyl-L-leucine methyl ester: selective ablation of cytotoxic lymphocytes and monocytes. J Immunol. 1986 Feb 1;136(3):1038-48. [Content Brief]

[2]. Thiele DL, et, al. Mechanism of L-leucyl-L-leucine methyl ester-mediated killing of cytotoxic lymphocytes: dependence on a lysosomal thiol protease, dipeptidyl peptidase I, that is enriched in these cells. Proc Natl Acad Sci U S A. 1990 Jan;87(1):83-7. [Content Brief]

[3]. Kalogeropulou AF, et, al. Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity. Biochem J. 2020 Nov 27;477(22):4397-4423. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9475 mL	14.7375 mL	29.4751 mL	73.6876 mL
	5 mM	0.5895 mL	2.9475 mL	5.8950 mL	14.7375 mL
	10 mM	0.2948 mL	1.4738 mL	2.9475 mL	7.3688 mL
	15 mM	0.1965 mL	0.9825 mL	1.9650 mL	4.9125 mL
	20 mM	0.1474 mL	0.7369 mL	1.4738 mL	3.6844 mL
	25 mM	0.1179 mL	0.5895 mL	1.1790 mL	2.9475 mL
	30 mM	0.0983 mL	0.4913 mL	0.9825 mL	2.4563 mL
	40 mM	0.0737 mL	0.3684 mL	0.7369 mL	1.8422 mL
	50 mM	0.0590 mL	0.2948 mL	0.5895 mL	1.4738 mL
	60 mM	0.0491 mL	0.2456 mL	0.4913 mL	1.2281 mL
	80 mM	0.0368 mL	0.1842 mL	0.3684 mL	0.9211 mL
	100 mM	0.0295 mL	0.1474 mL	0.2948 mL	0.7369 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-Leucyl-L-Leucine methyl ester hydrobromide Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Leucyl-L-Leucine methyl ester16689-14-8LLOMeLeu-Leu methyl esterH-Leu-Leu-OMeEndogenous MetabolitemonocytespolymorphonuclearleukocyteslymphocytescytotoxicendolysosomalpathwaystressInhibitorinhibitorinhibit

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L-Leucyl-L-Leucine methyl ester hydrobromide (Synonyms: LLOMe hydrobromide; Leu-Leu methyl ester hydrobromide; H-Leu-Leu-OMe hydrobromide)

Customer Review

Other Forms of L-Leucyl-L-Leucine methyl ester hydrobromide:

L-Leucyl-L-Leucine methyl ester hydrobromide Related Antibodies

18 Publications Citing Use of MCE L-Leucyl-L-Leucine methyl ester hydrobromide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

L-Leucyl-L-Leucine methyl ester hydrobromide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

L-Leucyl-L-Leucine methyl ester hydrobromide Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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