1. Metabolic Enzyme/Protease
  2. Proteasome
    HIV Protease

Pepstatin (Synonyms: Pepstatin A)

Cat. No.: HY-P0018 Purity: 98.45%
Handling Instructions

Pepstatin is a specific aspartic proteases inhibitor produced by actinomycetes, and also inhibits HIV protease. Sequence: Isovaleryl-Val-Val-Sta-Ala-Sta-OH.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Pepstatin Chemical Structure

Pepstatin Chemical Structure

CAS No. : 26305-03-3

Size Price Stock Quantity
10 mg USD 60 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
50 mg USD 204 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Other Forms of Pepstatin:

    Pepstatin purchased from MCE. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948.

    Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Pepstatin is a specific aspartic proteases inhibitor produced by actinomycetes, and also inhibits HIV protease. Sequence: Isovaleryl-Val-Val-Sta-Ala-Sta-OH.

    IC50 & Target

    IC50: 4.5 nM (Hemoglobin-pepsin), 6.2 nM (Hemoglobin-proctase), 150 nM (Casein-pepsin), 260 nM (Hemoglobin-acid protease), 290 nM (Casein-proctase), 520 nM (Casein-acid protease)[1]

    In Vitro

    Pepstatin is a specific acid protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively[1]. Pepstatin (Pepstatin A) inhibits the recombinant HIV protease with an IC50 of 250 μM. Pepstatin shows no effect on cellular protein synthesis and probably does not exert severe cell toxicity[2].

    In Vivo

    Pepstatin has a very low toxicity, with LD50s of 1090 mg/kg, 875 mg/kg, 820 mg/kg and 450 mg/kg for mice, rats, rabbits, and dogs by i.p. route, and > 2000 mg/kg for all species by oral route. Pepstatin (0.5-50 mg/kg, p.o.) suppresses stomach ulceration of the pylorus in ligated Shay rats[1].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 25 mg/mL (36.45 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4580 mL 7.2898 mL 14.5796 mL
    5 mM 0.2916 mL 1.4580 mL 2.9159 mL
    10 mM 0.1458 mL 0.7290 mL 1.4580 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [2]

    Pepstatin A is freshly dissolved in DMSO at 7 mM. It is very slowly diluted (1:100) into the medium of HIV-infected H9 suspension cultures so that no pepstatin A precipitated (final concentration, 70 μM pepstatin A and 1% DMSO), and the cultures are incubated without change of culture medium for 48 hr. As control, uninfected H9 cells are also incubated with pepstatin and in addition HIV infected and uninfected cells are incubated with 1% DMSO but without pepstatin[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    685.89

    Formula

    C₃₄H₆₃N₅O₉

    CAS No.

    26305-03-3

    Storage
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 98.45%

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    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

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    Product Name:
    Pepstatin
    Cat. No.:
    HY-P0018
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    Pepstatin

    Cat. No.: HY-P0018