1. Metabolic Enzyme/Protease
  2. Proteasome
    HIV Protease

Pepstatin (Synonyms: Pepstatin A; Isovaleryl-Val-Val-Sta-Ala-Sta-OH)

Cat. No.: HY-P0018 Purity: 98.45%
Handling Instructions

Pepstatin is a specific aspartic proteases inhibitor produced by actinomycetes, and also inhibits HIV protease.

For research use only. We do not sell to patients.
Pepstatin Chemical Structure

Pepstatin Chemical Structure

CAS No. : 26305-03-3

Size Price Stock Quantity
10 mg USD 60 In-stock
50 mg USD 204 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Other Forms of Pepstatin:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Pepstatin is a specific aspartic proteases inhibitor produced by actinomycetes, and also inhibits HIV protease.

IC50 & Target

IC50: 4.5 nM (Hemoglobin-pepsin), 6.2 nM (Hemoglobin-proctase), 150 nM (Casein-pepsin), 260 nM (Hemoglobin-acid protease), 290 nM (Casein-proctase), 520 nM (Casein-acid protease)[1]

In Vitro

Pepstatin is a specific acid protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively[1]. Pepstatin (Pepstatin A) inhibits the recombinant HIV protease with an IC50 of 250 μM. Pepstatin shows no effect on cellular protein synthesis and probably does not exert severe cell toxicity[2].

In Vivo

Pepstatin has a very low toxicity, with LD50s of 1090 mg/kg, 875 mg/kg, 820 mg/kg and 450 mg/kg for mice, rats, rabbits, and dogs by i.p. route, and > 2000 mg/kg for all species by oral route. Pepstatin (0.5-50 mg/kg, p.o.) suppresses stomach ulceration of the pylorus in ligated Shay rats[1].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.4580 mL 7.2898 mL 14.5796 mL
5 mM 0.2916 mL 1.4580 mL 2.9159 mL
10 mM 0.1458 mL 0.7290 mL 1.4580 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Pepstatin is dissolved in DMSO, and the diluted (1:100) into the medium.

Pepstatin A is freshly dissolved in DMSO at 7 mM. It is very slowly diluted (1:100) into the medium of HIV-infected H9 suspension cultures so that no pepstatin A precipitated (final concentration, 70 μM pepstatin A and 1% DMSO), and the cultures are incubated without change of culture medium for 48 hr. As control, uninfected H9 cells are also incubated with pepstatin and in addition HIV infected and uninfected cells are incubated with 1% DMSO but without pepstatin[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







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Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 25 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 98.45%

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