2. Anti-infection Metabolic Enzyme/Protease Autophagy
  3. HIV Protease Autophagy
  4. Pepstatin

Pepstatin  (Synonyms: Pepstatin A)

Cat. No.: HY-P0018 Purity: 98.40%
COA Handling Instructions

Pepstatin (Pepstatin A) is a specific, orally active aspartic protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively. Pepstatin also inhibits HIV protease.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Pepstatin Chemical Structure

Pepstatin Chemical Structure

CAS No. : 26305-03-3

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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of Pepstatin:

Pepstatin Related Antibodies

Top Publications Citing Use of Products

    Pepstatin purchased from MCE. Usage Cited in: Pharmaceuticals. 2022, 15(12), 1509

    TSN (10 μM), Baf (0.5 μM), and E64D (25 μM) plus pepstatin A (50 μM; E + P ) treatment significantly inhibits the degradation ability of lysosomes (in HepG2 cells and L02 cells; medium for 6 h).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Pepstatin (Pepstatin A) is a specific, orally active aspartic protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively. Pepstatin also inhibits HIV protease[1][2].

    IC50 & Target

    IC50: 4.5 nM (Hemoglobin-pepsin), 6.2 nM (Hemoglobin-proctase), 150 nM (Casein-pepsin), 260 nM (Hemoglobin-acid protease), 290 nM (Casein-proctase), 520 nM (Casein-acid protease)[1]
    In Vitro

    Pepstatin (Pepstatin A) (7 μM; 48 h) affects the intracellular processing of HIV-specific gag protein[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Pepstatin Related Antibodies
    In Vivo

    Pepstatin (Pepstatin A) has a very low toxicity, with LD50s of 1090 mg/kg, 875 mg/kg, 820 mg/kg and 450 mg/kg for mice, rats, rabbits, and dogs by i.p. route, and > 2000 mg/kg for all species by oral route[1].
    ? Pepstatin (0.5-50 mg/kg, p.o.) suppresses stomach ulceration of the pylorus in ligated Shay rats[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Pylorus ligated male Wistar rats[1]
    Dosage: 0.5, 1, 10 and 50 mg/kg
    Administration: Oral administration, 15 minutes after pyloric ligation
    Result: Effectively prevented stomach ulceration.
    Molecular Weight

    685.89

    Appearance

    Solid

    Formula

    C34H63N5O9
    CAS No.
    Sequence

    IsoValeryl-Val-Val-Sta-Ala-Sta-OH
    Sequence Shortening

    IsoVeryl-VV-Sta-A-Sta-OH
    Structure Classification
    Initial Source

    actinomycetes
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 33.33 mg/mL (48.59 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4580 mL 7.2898 mL 14.5796 mL
    5 mM 0.2916 mL 1.4580 mL 2.9159 mL
    10 mM 0.1458 mL 0.7290 mL 1.4580 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 2.08 mg/mL (3.03 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (3.03 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (3.03 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 98.40%

    COA (255 KB) HNMR (313 KB) LCMS (257 KB) Elemental Analysis Report (112 KB)

    Handling Instructions (2659 KB)
    References
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    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Pepstatin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Pepstatin26305-03-3Pepstatin AHIV ProteaseAutophagyaspartic proteaseorally activehemoglobin-pepsinhemoglobin-proctasecasein-pepsincasein-proctasecasein-acid proteasehemoglobin-acid proteaseHIV proteaseInhibitorinhibitorinhibit

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