1. Metabolic Enzyme/Protease
  2. Proteasome
    HIV Protease

Pepstatin Ammonium (Synonyms: Pepstatin A Ammonium)

Cat. No.: HY-P0018B Purity: 99.35%
Handling Instructions

Pepstatin Ammonium is a specific aspartic protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively. Pepstatin Ammonium also inhibits HIV protease.

For research use only. We do not sell to patients.

Pepstatin Ammonium Chemical Structure

Pepstatin Ammonium Chemical Structure

Size Price Stock Quantity
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
25 mg USD 110 In-stock
Estimated Time of Arrival: December 31
50 mg USD 170 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Other Forms of Pepstatin Ammonium:

    Pepstatin Ammonium purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2018 Aug 4;356:159-171.

    Western blot analysis of PARP and cleaved PARP levels MIA PaCa-2 and PANC-1 cells pre-treated with inhibitors of lysosomal hydrolases (Aloxistatin, 5 μM) for 1 h, followed by a 24-h treatment with Alan (20 μM).

    Pepstatin Ammonium purchased from MCE. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948.

    Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.

    Pepstatin Ammonium purchased from MCE. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948.

    Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Pepstatin Ammonium is a specific aspartic protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively. Pepstatin Ammonium also inhibits HIV protease.

    IC50 & Target

    IC50: 4.5 nM (Hemoglobin-pepsin), 6.2 nM (Hemoglobin-proctase), 150 nM (Casein-pepsin), 260 nM (Hemoglobin-acid protease), 290 nM (Casein-proctase), 520 nM (Casein-acid protease)[1]

    In Vitro

    Pepstatin Ammonium is a specific acid protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively[1]. Pepstatin (Pepstatin A) inhibits the recombinant HIV protease with an IC50 of 250 μM. Pepstatin shows no effect on cellular protein synthesis and probably does not exert severe cell toxicity[2].

    In Vivo

    Pepstatin has a very low toxicity, with LD50s of 1090 mg/kg, 875 mg/kg, 820 mg/kg and 450 mg/kg for mice, rats, rabbits, and dogs by i.p. route, and >2000 mg/kg for all species by oral route. Pepstatin (0.5-50 mg/kg, p.o.) suppresses stomach ulceration of the pylorus in ligated Shay rats[1].

    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (59.28 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4226 mL 7.1132 mL 14.2264 mL
    5 mM 0.2845 mL 1.4226 mL 2.8453 mL
    10 mM 0.1423 mL 0.7113 mL 1.4226 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (2.96 mM); Clear solution

    References
    Cell Assay
    [2]

    Pepstatin A is freshly dissolved in DMSO at 7 mM. It is very slowly diluted (1:100) into the medium of HIV-infected H9 suspension cultures so that no pepstatin A precipitated (final concentration, 70 μM pepstatin A and 1% DMSO), and the cultures are incubated without change of culture medium for 48 hr. As control, uninfected H9 cells are also incubated with pepstatin and in addition HIV infected and uninfected cells are incubated with 1% DMSO but without pepstatin[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    702.92

    Formula

    C₃₄H₆₆N₆O₉

    SMILES

    CC(C[[email protected]](NC([[email protected]@H](NC([[email protected]@H](NC(CC(C)C)=O)C(C)C)=O)C(C)C)=O)[[email protected]@H](O)CC(N[[email protected]@H](C)C(N[[email protected]@H](CC(C)C)[[email protected]@H](O)CC(O)=O)=O)=O)C.N

    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

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    Product Name:
    Pepstatin Ammonium
    Cat. No.:
    HY-P0018B
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    Pepstatin Ammonium

    Cat. No.: HY-P0018B