Methyl methanesulfonate

Name: Methyl methanesulfonate
Brand: MedChemExpress
SKU: HY-W004702
Rating: 5.0 (3 reviews)

Cat. No.: HY-W004702 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Methyl methanesulfonate is an alkylating agent which transfers methyl groups, and induces DNA damage. Methyl methanesulfonate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

CAS No. : 66-27-3

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
5 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
500 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Methyl methanesulfonate:

Methyl methanesulfonate (Standard) In-stock
Methyl methanesulfonate-d₃ Get quote

3 Publications Citing Use of MCE Methyl methanesulfonate

Cell Proliferation/Viability Assay

: Methyl methanesulfonate purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2019 Oct;23(10):6797-6804. [Abstract]; Berberine sensitizes breast cancer cells (MDA-MB-231) to Methyl methanesulfonate (0-800 μmol/L).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	EC₅₀	2750 μM Compound: MMS	Cytotoxicity against human MCF7 cells after 16 hrs by MTT assay Cytotoxicity against human MCF7 cells after 16 hrs by MTT assay	[PMID: 21839641]

In Vitro

Methyl methanesulfonate (50-800 μM; 24 h) upregulates the generation of phosphorylated p53, RIPK and ROS, induces necroptosis in cell A549^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	A549
Concentration:	50-800 μM
Incubation Time:	24 h
Result:	Increased p53 phosphorylation and RIPK.

In Vivo

Methyl methanesulfonate (120 mg/kg, ip, single dose) induces DNA damage in mouse models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NMRI mouse model^[2]
Dosage:	120 mg/kg
Administration:	ip, single dose
Result:	Increased DNA with comet tails in mouse liver, lung, kidney, and spleen.

Molecular Weight

110.13

Formula

C₂H₆O₃S

CAS No.

66-27-3

Appearance

Liquid (Density: 1.3 g/cm³)

Color

Colorless to light yellow

SMILES

COS(C)(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (908.02 mM)

DMSO : 100 mg/mL (908.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	9.0802 mL	45.4009 mL	90.8018 mL
5 mM	1.8160 mL	9.0802 mL	18.1604 mL
10 mM	0.9080 mL	4.5401 mL	9.0802 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (282 KB) SDS (789 KB)

COA (257 KB) HNMR (192 KB) GC (176 KB)

Handling Instructions (2659 KB)

References

[1]. Jiang Y, et al., Methyl methanesulfonate induces necroptosis in human lung adenoma A549 cells through the PIG-3-reactive oxygen species pathway. Tumour Biol. 2016 Mar;37(3):3785-95. [Content Brief]

[2]. Hosseinzadeh H, et al., Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol. 2008 Dec;27(12):657-64. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	9.0802 mL	45.4009 mL	90.8018 mL	227.0045 mL
	5 mM	1.8160 mL	9.0802 mL	18.1604 mL	45.4009 mL
	10 mM	0.9080 mL	4.5401 mL	9.0802 mL	22.7004 mL
	15 mM	0.6053 mL	3.0267 mL	6.0535 mL	15.1336 mL
	20 mM	0.4540 mL	2.2700 mL	4.5401 mL	11.3502 mL
	25 mM	0.3632 mL	1.8160 mL	3.6321 mL	9.0802 mL
	30 mM	0.3027 mL	1.5134 mL	3.0267 mL	7.5668 mL
	40 mM	0.2270 mL	1.1350 mL	2.2700 mL	5.6751 mL
	50 mM	0.1816 mL	0.9080 mL	1.8160 mL	4.5401 mL
	60 mM	0.1513 mL	0.7567 mL	1.5134 mL	3.7834 mL
	80 mM	0.1135 mL	0.5675 mL	1.1350 mL	2.8376 mL
	100 mM	0.0908 mL	0.4540 mL	0.9080 mL	2.2700 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.