2. Others GPCR/G Protein
  3. Environmental Pollutants LPL Receptor
  4. Poloxamer 407 (F127)

Poloxamer 407 (F127)  (Synonyms: PEG-PPG-PEG, 12600 (Average Mn))

Cat. No.: HY-D1005
COA
SDS
Handling Instructions Technical Support

Poloxamer 407 (F127) is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8 AM and Quest Rhod-4 AM, etc. to improve their water solubility. Poloxamer 407 is also a lipoprotein lipase inhibitor.

For research use only. We do not sell to patients.

CAS No. : 9003-11-6

Size Price Stock Quantity
500 mg In-stock
1 g In-stock
5 g In-stock
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Poloxamer 407 (F127):

Poloxamer 407 (F127) Related Antibodies

Top Publications Citing Use of Products

    Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Feb 5;44(2):115246.  [Abstract]

    We observed a decrease in the serum TG levels after poloxamer 407 (0.2 g/mL, 200 μL, Intrauterine injection) administration as well as a decrease in the serum TG content in LKO-Met mice compared with those in L-WT mice fed an HFD.

    Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Feb 5;44(2):115246.  [Abstract]

    We observed a decrease in the serum TG levels after poloxamer 407 (0.2 g/mL, 200 μL, Intrauterine injection) administration as well as a decrease in the hepatic PC content in LKO-Met mice compared with those in L-WT mice fed an HFD.

    Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):21506.  [Abstract]

    Distribution of S100A8-positive cells in the endometrium of four different groups with estrogen treatment (control, injury, P407 (0.2 g/mL, 200 μL, Intrauterine injection), and S100A8 + P407 groups). Black arrows show S100A8-PICs. S100A8-PICs were distributed around the glands and blood vessels in the control group. S100A8-PICs in the site of injury or adhesion in the injury group and in the P407 group. S100A8-PICs reverse migrated to the glands or blood vessels in the S100A8 + P407 group. Scale bar is 100 μm or 1 mm.

    Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):21506.  [Abstract]

    Ki-67-positive proliferating cells were distributed in the endometrium or uterine interior wall in all groups (control, injury, P407 (0.2 g/mL, 200 μL, Intrauterine injection), and S100A8 + P407 groups). Scale bar is 100 or 400 μm.

    Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):21506.  [Abstract]

    Immunohistochemical staining showing Claudin-1 distribution in the endometrium of different groups (control, injury, P407 (0.2 g/mL, 200 μL, Intrauterine injection), and P407 + S100A8 groups); black arrows show Claudin-1 accumulated below the cell membrane of the lateral contact zone between adjacent cells and red arrows show cells expressing Claudin-1 in the stroma. Scale bar is 100 μm.

    View All LPL Receptor Isoform Specific Products:

    View All Isoforms
    LPAR1 LPAR2 LPAR3 LPAR5 S1PR1 S1PR2 S1PR3 S1PR4 S1PR5

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Poloxamer 407 (F127) is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8 AM and Quest Rhod-4 AM, etc. to improve their water solubility. Poloxamer 407 is also a lipoprotein lipase inhibitor[1][2].

    In Vitro

    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Dissolve 1 g of Poloxamer 407 (F127) in 10 mL distilled water to make a 10% (w/v) stock solution, or 2 g of Poloxamer 407 (F127) in 10 mL DMSO to make a 20% (w/v) stock solution. These may require heating from 40 to 50°C for about 30 minutes. Store solution at room temperature. Do not refrigerate or freeze the Poloxamer 407 (F127) solution since it may precipitate. If precipitation is observed, the precipitates can be dissolved by heating to 37°C and vortexing before use.
    2. Dilute the 10% or 20% Poloxamer 407 (F127) stock solution into the cell-loading buffer such as Hanks and 20 mM Hepes buffer (HHBS) at 1:1000 to 1:500 dilution to achieve a 0.02 to 0.04% working solution.
    3. The DMSO stock solution of AM ester is then diluted into the 0.02 to 0.04% working solution to achieve a final AM ester concentration between 1 μM and 10 μM. The final concentration of Poloxamer 407 (F127) is normally kept at or below 0.08%.
    4. The cells are incubated at a desired temperature for between 10 minutes and 1 hour. In general it is desirable to use the minimum amount of AM ester needed to achieve adequate fluorescence signal to noise levels.
    5. After labeling, the cells are washed with HHBS or fresh medium before starting the experiment.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Poloxamer 407 (F127) Related Antibodies
    In Vivo

    Poloxamer 407 (F127) (0.25 g/kg; i.p.; every other day for 7 weeks) induces hypertriglyceridemia but decreases atherosclerosis in Ldlr-/- mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ldlr−/− mice[2]
    Dosage: 0.25 g/kg
    Administration: Intraperitoneal injection, every other day for 7 weeks
    Result: Mice tended to have a lower body weight and had smaller epididymal fat pads compared to the saline controls, and had reduced atherosclerosis.
    Molecular Weight

    12600(Average)

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC.[H(OCH2CH2)x(OCH2CH)y(OCH2CH2)zOH].[CH3]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 110 mg/mL (Need ultrasonic)

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL; Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    SDS (251 KB)

    COA (243 KB)

    Handling Instructions (2659 KB)
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Poloxamer 407 (F127) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Poloxamer 407 (F127)9003-11-6PEG-PPG-PEG, 12600 (Average Mn)Environmental PollutantsLPL ReceptorEnvironmental ContaminantsLysophospholipid ReceptorInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Poloxamer 407 (F127)
    Cat. No.:
    HY-D1005
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.