Premafloxacin 

Premafloxacin is a potent antimicrobial agent that exhibits activity against Staphylococcus aureus, Corynebacterium bovis, and Corynebacterium amylocolatum. Premafloxacin demonstrated potent antimicrobial activity against S. aureus by targeting topoisomerase IV, and is a poor substrate for NorA efflux pump. Premafloxacin can be used for antimicrobial research^[1][2].

Premafloxacin (24-48 h) exhibits activity against wild-type S. aureus ISP794 with MIC of 0.004-0.008 μg/mL, while showing 4- to 8-fold decreased activity against various grlA and grlB mutants of these two strains with altered subunits of topoisomrase IV (MIC: 0.032-0.064 μg/mL)^[1].
Premafloxacin (2-8× MIC; 48 h) fails to select for resistant single-step mutants of S. aureus ISP794 at 4 × or higher MICs, with resistant mutants only detectable at 2× MIC (mutation frequency: 6.4×10^-10 to 4.0×10^-7)^[1].
Premafloxacin (24 h) exhibits potent in vitro activity against both Corynebacterium bovis and Corynebacterium amylocolatum strains isolated from bovine mammary glands, with MIC₉₀s of 0.015 μg/mL^[2].
Premafloxacin (24-48 h) exhibits a 2-fold decreased activity against flqB mutant, which overexpresses NorA efflux pump, indicating that it is a poor substrate for NorA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

403.45

C₂₁H₂₆FN₃O₄

143383-65-7

O(C)C1=C2N(C=C(C(O)=O)C(=O)C2=CC(F)=C1N3C[C@]([C@@H](NC)C)(CC3)[H])C4CC4

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ince D, et al. Mechanisms and frequency of resistance to premafloxacin in Staphylococcus aureus: novel mutations suggest novel drug-target interactions. Antimicrob Agents Chemother. 2000;44(12):3344-3350.  [Content Brief]

  [2]. Watts JL, et al. Susceptibilities of Corynebacterium bovis and Corynebacterium amylocolatum isolates from bovine mammary glands to 15 antimicrobial agents. Antimicrob Agents Chemother. 2000;44(12):3476-3477.  [Content Brief]